FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003626372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003626372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences233
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2410.300429184549357Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT198.15450643776824Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC83.4334763948497855Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG52.1459227467811157Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG52.1459227467811157Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA41.7167381974248928No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT41.7167381974248928Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG41.7167381974248928Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT41.7167381974248928Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA41.7167381974248928Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC41.7167381974248928Clontech SMART CDS Primer II A (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG41.7167381974248928Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT41.7167381974248928Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31.2875536480686696No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC31.2875536480686696Clontech Universal Primer Mix Long (96% over 26bp)
CCTCTGGACTCTTGCCCGCAGCCTGGAGCTCCTTTCGGGCCTCCTCCGTC31.2875536480686696No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31.2875536480686696No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA31.2875536480686696Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC31.2875536480686696Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA31.2875536480686696Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCCATTGCTGAGGCCTGGGCTCGCCTGGACCACAAGTTTGACCTGATGT20.8583690987124464No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGAT20.8583690987124464No Hit
CCACTGCTTCCCTGTACTCTGCGTTGATACCACTGCTTCCCTGTACTCTG20.8583690987124464Clontech Universal Primer Mix Long (96% over 26bp)
TCTCGCCCGAGGACGCCGGCCGCTTTGGCCGCGCGAGCGATCGCACCGGG20.8583690987124464No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC20.8583690987124464Clontech Universal Primer Mix Long (96% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTATAC20.8583690987124464Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.8583690987124464No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT20.8583690987124464Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA20.8583690987124464Clontech Universal Primer Mix Long (95% over 21bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.4291845493562232No Hit
GTACAGGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCTCTTATAC10.4291845493562232Clontech Universal Primer Mix Long (96% over 28bp)
TAGTCCATGGCGTAGAAGCCGCCGCCGCCCCATCTGCTGCAGTCTGTCTC10.4291845493562232No Hit
ATCCACCTTCGACCCTTAAGTTTCATAAGGGCTATCGTAGTTTTCTGGGG10.4291845493562232No Hit
GCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAG10.4291845493562232Clontech Universal Primer Mix Long (96% over 25bp)
GGTCATCGTTACGCGTGTCAGCACCGATCAGGATGTTGGCGAGCAGATGG10.4291845493562232No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA10.4291845493562232Clontech Universal Primer Mix Long (96% over 26bp)
GGATTAAGGCGACAGCGATTTCTAGGATAGTCAGTAGAATTAGAATTGTG10.4291845493562232No Hit
GAGCAGGTCGACCTGGCGATGCTGGTGAGCACCAGCGCGATTCCGCACGT10.4291845493562232No Hit
GAATGATATTTGGCCTCACGGGAGGACATAGCCTATGAAGGCTGTTGCTA10.4291845493562232No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTG10.4291845493562232No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGCTGTCTCTTATAC10.4291845493562232Clontech SMART CDS Primer II A (100% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCATCCCATGTACTC10.4291845493562232Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATC10.4291845493562232Clontech SMART CDS Primer II A (100% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA10.4291845493562232No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACCTGTCTCTT10.4291845493562232Clontech Universal Primer Mix Long (96% over 26bp)
GCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA10.4291845493562232Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA10.4291845493562232Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACAGGGGAAGCAGTGGTATCAACGCAGAGTACAT10.4291845493562232Clontech Universal Primer Mix Long (96% over 28bp)
TATCAACGCAGAGTAAATGGGAAGCAGTGGTATCAACGCAGAGTACATGT10.4291845493562232Clontech Universal Primer Mix Long (96% over 26bp)
GAAAGCAAAGTATCTTGGCCTCCTTCTTCCACCCCTCTATATCCACTCTG10.4291845493562232No Hit
CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA10.4291845493562232Clontech Universal Primer Mix Long (95% over 22bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA10.4291845493562232Clontech Universal Primer Mix Long (95% over 21bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCCTGTCTCT10.4291845493562232Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA10.4291845493562232Clontech Universal Primer Mix Long (95% over 21bp)
GCCGTAGTCGGTGTACTCGTAGGTTCAGTACCATTGGTGGCCAATTGATT10.4291845493562232No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTCTGT10.4291845493562232Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTC10.4291845493562232Clontech Universal Primer Mix Long (96% over 26bp)
CCTCATGGGCTACACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTT10.4291845493562232No Hit
ACCTCGAGGAAGTCTCCCTTGAGCAGGCCCTCGTAGCCGTGCTTGTAGGC10.4291845493562232No Hit
CTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCA10.4291845493562232Clontech SMART CDS Primer II A (100% over 26bp)
GGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAGGGT10.4291845493562232No Hit
CTCATGTACTCTGCGTTGATACCACTGCTTCTCATGTACTCTGCGTTGAT10.4291845493562232Clontech SMART CDS Primer II A (96% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAATTAATCTGCGTTGATACCACTGCTT10.4291845493562232Clontech Universal Primer Mix Long (95% over 23bp)
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATAATGATTCCC10.4291845493562232No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC10.4291845493562232Clontech Universal Primer Mix Long (96% over 26bp)
TTTTTCTAGGGCGCGAAAAAGCCAGCACATCTTTTTCTTCTACCCACCCG10.4291845493562232No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC10.4291845493562232Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACATGGGAAGCAGTGGTATCAACGAAGAGTACATTGAAAGAAATGTAAAA10.4291845493562232Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTTGGGGGCTCTGCATGCCCAGGCCCAGGACTCCACCTCAGACCTGATCC10.4291845493562232No Hit
CTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATA10.4291845493562232No Hit
GACATCTGCGGCGAGGGAACTCCCCAGGCCACCGGGTTCTTTGAAGTGAT10.4291845493562232No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT10.4291845493562232Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC10.4291845493562232Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCTAAAAAAAAAAAAAAAAAAAAAA10.4291845493562232No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.4291845493562232No Hit
GGTTACAGAGTGTGCTAATGACAGCCAGATAACTGCGCTGTTAAATTACA10.4291845493562232No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA10.4291845493562232Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGC10.4291845493562232Clontech Universal Primer Mix Long (95% over 22bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT10.4291845493562232Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTAAGAAGTCATTGGCTGAGCGGCGGGCTTTAATCACACGATGCGCTTT10.4291845493562232No Hit
GTATCAGTGTCTACAACCAGAGAGCCCATAAGTAGTGAGCACTTGAACAG10.4291845493562232No Hit
ATTAGCATACACTGTGTGTCCTGTAAGGACGGCTGTTGGACAAAGCAGGA10.4291845493562232No Hit
CAACACAGAGTACTGGGAAGCAGTGGTATCAACGCAGAGTACTGGGAAGC10.4291845493562232Clontech Universal Primer Mix Long (96% over 26bp)
GAGGTTTTTCGATAACACAGCTAAATGTTATTAATTCAAGAATTTGCTAG10.4291845493562232No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA10.4291845493562232Clontech Universal Primer Mix Long (96% over 26bp)
NTACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCGGCCCGTGTA10.4291845493562232Clontech SMART CDS Primer II A (95% over 24bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGTAT10.4291845493562232Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT10.4291845493562232Clontech Universal Primer Mix Long (96% over 26bp)
ATGTAATCTTTGATGTACTTCTTGTAGGCTTCTTTTGTGAAACTCGTTTC10.4291845493562232No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTA10.4291845493562232Clontech SMART CDS Primer II A (100% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATC10.4291845493562232Clontech Universal Primer Mix Long (96% over 26bp)
AGAGAAGACATATTCTACACTTCAAAGCTTTGGAGCAATTCCCATCGACC10.4291845493562232No Hit
ACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAGTTA10.4291845493562232Clontech Universal Primer Mix Long (95% over 22bp)
TAACTATCTTGGGCTGTGACAAAGTCACATGGTTCACACGGCAGGCATAC10.4291845493562232No Hit
TCTCTGTACCATGGCTCGTACAAAGCAGACTGCCCGCAAATCGACCGGTG10.4291845493562232No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.4291845493562232Clontech SMART CDS Primer II A (100% over 23bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGCGTCCCATGT10.4291845493562232Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTGTGTTGATACCACTGCTTCCCATGTACTCTGCG10.4291845493562232No Hit
GGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAATGGGT10.4291845493562232No Hit
GTTCTGGCGAGCAGTTTTGTTGATTTAACTGTTGAGGTTTAGGGCTAAGC10.4291845493562232No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGT10.4291845493562232Clontech Universal Primer Mix Long (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC10.4291845493562232Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAATAATGAAGGGCAAGATGAAGTGAAAGGTAAAGAATCGTGTGAGGGTG10.4291845493562232No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAATATGCGTTTATACCAC10.4291845493562232No Hit
GCCCTGTCCAACATGAGCCAGGACATGATCAGGATCGACCTGCAGGACGG10.4291845493562232No Hit
CCACCACACCAGGCTAATTTTGTATTTTTAGTAGAGACGGGGTTTCACCA10.4291845493562232No Hit
CAGTGACACCATCGAAAATGTGAAGGCCAAGATCCAGGATAAAGAAGGCA10.4291845493562232No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA10.4291845493562232Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACTTTT10.4291845493562232Clontech SMART CDS Primer II A (100% over 26bp)
TGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAATTAAAACTGG10.4291845493562232Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGCAGCGGTGGTGTCAACGTGCAAGAGGAGTGACTCAAAAGTA10.4291845493562232No Hit
TCTCGTACGACCCCACCCGTGAGCTGTACCAGGATTACAACGCTGAGTTC10.4291845493562232No Hit
GGCTATAGCAATAAAACTAGGAATAGCCCCCTTTCACTTCTGAGTCCCAG10.4291845493562232No Hit
GTGGTATAAACGCAGAGTACATGGGAAGAATAGCAATCAACGCAGACTAC10.4291845493562232No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGATTACATGGGAAGAAGTGG10.4291845493562232Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTATACCTGTCTCTTATAC10.4291845493562232No Hit
TCTTAATCCAGCACCAGCAACGAAGGACATTCCAGCCGAGTTGTTCGCCG10.4291845493562232No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGAA10.4291845493562232Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT10.4291845493562232Clontech Universal Primer Mix Long (96% over 25bp)
ACCCTCGAGGTCGTCGAGAAAGTCCCGGGCCTGCAGGTTGTCCAAGCCAC10.4291845493562232No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.4291845493562232No Hit
ATTCTTTACCTTTCACTTCATCTTGCCCTTCATTATTGCAGCCCTAGCAG10.4291845493562232No Hit
GAGTACATGGGAAGCCGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10.4291845493562232Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGGGTGGAATTCGGGAACGGCCATTGTGGCGCACTTGTTCCGCCTGGAC10.4291845493562232No Hit
CACGATAACTTTTCTTCCCATATAAGCATGGGCTATCCTAGATCCCAGTC10.4291845493562232No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC10.4291845493562232Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAGTAC10.4291845493562232Clontech Universal Primer Mix Long (95% over 22bp)
TCTCGTACGACCCCACCCGTTAGCTGTACCAGGATTACAACGCTGAGTTC10.4291845493562232No Hit
GACGCACGATTTGAACGCGCAGTTGAAAGGCAATTTGGATATTCAGCCGC10.4291845493562232No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTG10.4291845493562232Clontech Universal Primer Mix Long (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)