FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003626405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003626405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA44.3478260869565215Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAGCTGCCTTTAATGCACTTGGAATTACTTCTTTGAAAAATACAAGATG33.260869565217391No Hit
GTCGTCAACGACCTGGGCAATCGCATGTTTCGCCCAGGCCGTGATCTGCT33.260869565217391No Hit
GCACATAGTCAGCCTGCTTGATCACGTCGATCTTGTCCAGGAAGTACTGG33.260869565217391No Hit
GCTATCAATCTGTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCG22.1739130434782608No Hit
CATGAGTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCA22.1739130434782608No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT22.1739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT22.1739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
CTCTCAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC22.1739130434782608No Hit
CATCGTGACTAAACAGGTACTGCTGGGCCGGAAGGTGGTGGTCGTACGCT22.1739130434782608No Hit
GATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACG22.1739130434782608No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC22.1739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
CTTCTGCCAGCAGCTCCTTGACCTTGCGCAGAAGGCCCACCTCCAGTGGC22.1739130434782608No Hit
CTCGTCAACGACCTGGGCAATCGCATGTTTCGCACAGGCCGTGATCTGCT11.0869565217391304No Hit
AACTTCTTCTAATTGTTCTTTAAGTTTTTCAGCATCTTCTTTAGGCATAG11.0869565217391304No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTGT11.0869565217391304No Hit
CTTCCAGGCCAAGCCCGCAGTGCCTACGTCTAAGCTGGGCCAGGCAGAGG11.0869565217391304No Hit
GATCTCGGCTCACTGCAACCTCCTGGGTTCAAGCAATTCTCCTAACCTCA11.0869565217391304No Hit
GCCGACAGGAGAATGGCTGCCGCGAGACTCTGAGTGCCACCTCTGCATGT11.0869565217391304No Hit
GACCAGGGTCACTTGCTGCATGTCCAGGCAGAAGATGGAGTAGAGCAGCT11.0869565217391304No Hit
GATTACAGGTGCCCTCCAACATGCCCAGCTAATTTTTGTATTTTTAGTAG11.0869565217391304No Hit
GAGTACATGGGAGGCTGCAGAGGTGCCATGGCTGAGTCACACCTGCTGCA11.0869565217391304No Hit
TTCTTATCGTGTTGGTGGTGGTGGTCCTCATCGTCTTGGTGATTGTGGGA11.0869565217391304No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTT11.0869565217391304No Hit
GTCAGGTAGCGTGATGCCTTCAGCTTTGTTCTTTTTGCTTACGATTATCT11.0869565217391304No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG11.0869565217391304Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCCTGGACTGTGTGACTTTACGGCAAACATACTTCGTTGCGAGCTGTTT11.0869565217391304No Hit
TCTCGAAGTAGTAGGCGACATCGCCGATATCCACGTCACCGGCGGCCTTC11.0869565217391304No Hit
CTATTCTGATTTTTCGGTCACCCTGAAGTTTATATTCTTATCCTACCAGG11.0869565217391304No Hit
ATGCTGAGCCAAGCTGGACCAAATTCTCTTTCATGATAATTTGGAACTAA11.0869565217391304No Hit
TTTTTAATTAGCTTGAAGGAAGCAATCACTGGGGAGAAAGCGGAAATCAA11.0869565217391304No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC11.0869565217391304Clontech Universal Primer Mix Long (96% over 26bp)
ATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTGCCCAC11.0869565217391304No Hit
GGCTAAGTGTGAGTGTCATTTCCTCAATGGGACGGAGCGAGTGTGGAACC11.0869565217391304No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG11.0869565217391304Clontech Universal Primer Mix Long (96% over 26bp)
ACTAACACAGGTGTACTTTCTTGATCTAGCACTAAATAATTTAATACTGT11.0869565217391304No Hit
GTTTAAGATCCTGCTTCTACTGATAACAACAAAAAAACTTGAGCAAATTA11.0869565217391304No Hit
GCTATCAATCTGTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTGTCCCG11.0869565217391304No Hit
TCCATCCCCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTGCCGAG11.0869565217391304No Hit
CAGCGCACCCGCAGCAGCGCCCGCAGCTCGTCCGCGCCATGTTCCAGGCG11.0869565217391304No Hit
GCATTGTTTCTAGGAGAACCCGCGTCATACCTTTATCTATAGCCTTCCCC11.0869565217391304No Hit
GAGTTGAACCTGACAAAGAGGTTGAAGTTGTTACTAATTCTGGATTAAGA11.0869565217391304No Hit
CCACGAGGCCAGTGGAGTCGATGGAGAAGGTGGCAGTGGTAACCAGGTGC11.0869565217391304No Hit
ATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCA11.0869565217391304No Hit
CTGGGGATCTTTCTTCACAAATCAGAGCTGGGCTGCGATACTGGGAGTAC11.0869565217391304No Hit
CTCTCGTACTGAGCAGAATTCACCAAGCGTTGAACAATCCAACGCTTGGT11.0869565217391304No Hit
CTGTGGGATTATGACTGAACGCCTCTAAGTCAGAATCCCACAGATGGTAG11.0869565217391304No Hit
AGTCCATTCGATGATTCCATTCGATTCCATTCAATGATGATTCCATTCGA11.0869565217391304No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11.0869565217391304Clontech Universal Primer Mix Long (96% over 26bp)
GGCTGGACCATGTGCCGGCGGATGTAGCGGATCCGCAGCAGCACCAGGGC11.0869565217391304No Hit
GTACATGGGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCA11.0869565217391304No Hit
CTCTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGT11.0869565217391304No Hit
CCCCAGCCCGACCGACCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTA11.0869565217391304No Hit
AGGCCGAGGCAGGCAGATCACGAGGTTAGGAGATCGAGACCATCCTGGCT11.0869565217391304No Hit
GATTTGGGTGGGAACACAACAAACCATATCAATTGTTTAGCACAGAAAAA11.0869565217391304No Hit
GAGTACTGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACACATCTCCG11.0869565217391304No Hit
CATGGGAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAA11.0869565217391304No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTA11.0869565217391304No Hit
CTCGGGGACAGTGCCAGGTGGGGAGTTTGACTGGGGCGGTACACCTGTCA11.0869565217391304No Hit
GCACAGCCCCGCAGCAGCCCAGGAAGCCCACCAGCATCATGAGGGCGCCG11.0869565217391304No Hit
TTTTGTACACGGGAGAGTGATCCAGTCCGTGGCCCTGACGGCAGGATGCA11.0869565217391304No Hit
GTGTCCTGGATGTGCTGCGCCACAAACTGCGACACGTCCGGCCTGTCTCT11.0869565217391304No Hit
TACGCCGCGGGCGACGGCGACGCGGCACTTGAGCTGGGACGCCTCTTCCT11.0869565217391304No Hit
GATATCTCGTGGGCTTTTAGGATGTTATAGCTACAGACCATTTTCATAAT11.0869565217391304No Hit
GTCGAGGCATGCGAAGACCGGCTGCGACTGCAGCGTCTGCGCGATTACGC11.0869565217391304No Hit
CTATAAAACCATCTTCTAAAGAGGACCAAAACAAGACAGTAATTGTCCAT11.0869565217391304No Hit
TTCCTAAGCCACTGGGAAGGAAGCCAGTCCCAGAAAAGAGGCTGTCTCTT11.0869565217391304No Hit
CCTCTGACCTCTCCCCTACCTCATGTTAACACTTGGAATTAAGTACCTGT11.0869565217391304No Hit
CAGTGACAGAGAAATGCACACTATGTATCAAATAGCAAGGAAATGAAGCA11.0869565217391304No Hit
ATGGCAAACTAATTGTTGAAGGAATGCTGGACATTTTCTGGGGAGTAAAA11.0869565217391304No Hit
AAGTCTCACTCTGTCGCCTAGGCTGGAGTGCAGTGGCGGAATCTTAGCTC11.0869565217391304No Hit
GCCATGGCCTCCCAAAGTGCTGAGATTACAGACATGAGCCACCACACGGG11.0869565217391304No Hit
ATTGTATCCCCAGCATTTATCAAAATGCCTCATATTTTGTAGGCACTCAA11.0869565217391304No Hit
AGCAATGGCTGCGGCCACCTCCACCAACATGATAAGAGACAGAAAGATGG11.0869565217391304No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)