FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003626416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003626416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGG33.4482758620689653No Hit
TCGTCGATCTCCCCCGCGGCGAAGCGACGGCCGAGCAGCGCGATCGGGGA33.4482758620689653No Hit
GCCTTGGGGACTAATAAACCTCACAACCTGAGGATCACCTCAGTTAAACC33.4482758620689653No Hit
CTGTTCTCCTGCTTGATCACGAGCGGCCGGTGGAAGCCGCAGAGTTCCTG33.4482758620689653No Hit
GCGCTCGGCGTGCCCGACGCCTACGCGGCCTGCGAGAAGATCAAGGCCGC22.2988505747126435No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA22.2988505747126435Clontech Universal Primer Mix Long (96% over 26bp)
CTCCTAGACGATGGAACGGGCATCCCGACCATAGCGTCGCCCGCCGCGCA22.2988505747126435No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT22.2988505747126435Clontech Universal Primer Mix Long (96% over 26bp)
GATTTACCCTACAGCTTCAGGCCCAGCTGCCCCCCAGTCTTTGGGTGGTG22.2988505747126435No Hit
GTCAGGGCCCCCAGCAGGGCCTTCAGGGCCTTCACGGCCCCCACGGCCTG22.2988505747126435No Hit
CTGCGGCTTAATTTGACTCAACACGGGAAACCTCACCCGGCCCGGACACG22.2988505747126435No Hit
TAGGCAGACTGCAGATCTGTAACTCTTTCTTCTGTTCACTAAATTAAGTG22.2988505747126435No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCAC22.2988505747126435No Hit
TCTCTATAATCTCTTGGTCCCATTTTAAAGATTAGGAAACATGCTCAGAA11.1494252873563218No Hit
ACGTTTACCTGTCCATGTCTTACTACTTTGACCGCGATGATGTGGCTTTG11.1494252873563218No Hit
CTGCTCGAGGGAAAGGAAGATCAATACAACAATTTGTCAGCCTCCAAGGT11.1494252873563218No Hit
GATTGGTTCCTGAGAGCCCCGAGAAGAAAATTCATGACAGTGTCTGGGCT11.1494252873563218No Hit
GAATGGAATCAACCAGAGTGGAATGGAAGGGAATGGAATGGAATGGAAAC11.1494252873563218No Hit
GGTGAATGAGAAACGCCGGGCTGCGCTGAATATGCTCTATATCGACCCGC11.1494252873563218No Hit
ACAGATAACCCAGAAATAAAGCCAAATGTTTATGACCAACTGATCTTTAA11.1494252873563218No Hit
CGGCCTCAGGCACTGTTCAGACAAATGAGGGATGGAGATGTGACAAGAGA11.1494252873563218No Hit
GGCGGCCGGGCGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGC11.1494252873563218No Hit
ATTTAACCCTGAGTGGACACAGCACATGTTTCAGAGAGCACAGGGTTGGG11.1494252873563218No Hit
GAGCAAACACATTCAAAAGCTAGCAGAAGGCAAGAAATAACTAAGATCAG11.1494252873563218No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG11.1494252873563218No Hit
CCTCCCGCCGCCCAAGATGCCGAAAGGAAAGAAGGCCAAGGGAAAGAAGG11.1494252873563218No Hit
ACCCAGTTCAATTTTTTTTTTATTTAAGTAGAGACAAAGTCTTGCCACGT11.1494252873563218No Hit
AACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAAT11.1494252873563218No Hit
GAATTTCTGTTCATGAAGTTTGTGATTCCACATCAAGATAATTCTTTATG11.1494252873563218No Hit
CCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATC11.1494252873563218No Hit
CTCAGGAGTAGCTGGGATTACAAGTGTGAGACACCGCGCCCTGCAATTTT11.1494252873563218No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAT11.1494252873563218No Hit
CTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCT11.1494252873563218No Hit
ATCTTCAAGAACTCTTGGGATCCAGCTCAATTTGGGAAGCCCCAGGCTCC11.1494252873563218No Hit
AGATCGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGA11.1494252873563218No Hit
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGC11.1494252873563218No Hit
AATTAGCTCTTTGCTGCCATTAAGAATTTTTTTTAATGTTTCATTTAGAC11.1494252873563218No Hit
GGGTTGAGGAGCCGGGCCTGGGCCCTGCGCACCTCCCGGGGCAGCTCGCC11.1494252873563218No Hit
GAATAACACTGCTGCAGATGCTTGTCATCTGCAGCAGTGTTATTCCACAT11.1494252873563218No Hit
GCGCATTTCCGCAAGCTGGCCGAGCACGTCAAGCGAGGCGTTTATGAGGC11.1494252873563218No Hit
TTTTTTTTTTTTTTTTTTTTTTTGGGTTGTTAACACTGCGTGCGGTGTGT11.1494252873563218No Hit
CCCCAGGCCGTGTAGATGGAGAAGGACACACAGCTGCAGCGGGGCCCACT11.1494252873563218No Hit
GGAGCGAGCTGGACGCCACTGTGGGGCTCTGAGAGTCCTGAATTCTTACT11.1494252873563218No Hit
GGCAATGCGCGCCTCGATCGGCGAGCCGAGCACGGCGATGGTGATGGTGT11.1494252873563218No Hit
TGCCTTTACCACTCAGAGAAGGAGCTGTGGTAGTGGCACCAGAATGGATT11.1494252873563218No Hit
CCTCGGACACCTTCTCGGCTGCCCGCTTCTCGGCCTGCAGCAGCTGCTGA11.1494252873563218No Hit
GTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCAT11.1494252873563218No Hit
CGCGGGAGGGCGCGTGCCCCGCCGCGCGCCGGGACCGGGGTCCGGTGCGG11.1494252873563218No Hit
AGACTATGGTGAGCTCAGGTGATTGATACTCCTGATGCGAGTAATACGGA11.1494252873563218No Hit
AAGAATATCTAGCCACATTTGCAGGAAACCTTTCCCTTAAGAAGCAGCTT11.1494252873563218No Hit
GTCATCAGCAGCCAGACGGGCATTGTCAATCTGTAGCAGGATGTTGGCAT11.1494252873563218No Hit
GTGTCATCCAGAGAGAGGGCTTCAGAGATTGGCAACGGTGGAAGAGGCCT11.1494252873563218No Hit
CCATTGCATTCCATTCCATTCGATTCCATTCGATTGCACTCGTGTTGACT11.1494252873563218No Hit
ACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTG11.1494252873563218No Hit
GAGATGATGAGATGAAATGAGATGAAAAGATGAAATGATGAGATGAAATG11.1494252873563218No Hit
TGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTA11.1494252873563218Clontech SMART CDS Primer II A (96% over 26bp)
TCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCA11.1494252873563218No Hit
ACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACC11.1494252873563218No Hit
CCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGCCGCCGCATCGCCGGT11.1494252873563218No Hit
GTCTGCGCGAGGGCCACGGCCGAGACGGCCAGGGCCAGCGCGGCCGCGGT11.1494252873563218No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGAC11.1494252873563218No Hit
GCTCTACTCAGCGCTCTCGGCGCCGGGTGCGGTGCACATGCGGGTCAAGG11.1494252873563218No Hit
ATATAGGCATGAGCCACTGAGCCTGGCCCAGAAGCGTTTTTCTCAAAGGC11.1494252873563218No Hit
TCTCTGGAGTGATAGGCTTCTGTTTGTTCTTGGCAAGTTTCTCAATAGTA11.1494252873563218No Hit
AGACCATCCTTTATTGTAGTATTAGTTCATGGTAACTGCATGAAAAAACA11.1494252873563218No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC11.1494252873563218No Hit
GTACTTGGGAAGCAGTGGTATAAACGCAGAGTACATGGGAAGCAGTGGTA11.1494252873563218Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTT11.1494252873563218No Hit
CCCAAGCCGTGTTCTCTTCTTGGGAGGAAGATCTTTTGCCTCCTCAGCCC11.1494252873563218No Hit
GTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGAGGGAG11.1494252873563218No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)