FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003626601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003626601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153
Sequences flagged as poor quality0
Sequence length100
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAGGACTTGCCCGGCCAGCCCGGGAGGTACGCGCTCTTCTTCGAGCGC42.6143790849673203No Hit
CGCTACCACCGGGGCGAGCTGGCGCGTGTCGCCGCTCAGGACCAGCTTCG31.9607843137254901No Hit
CCCGGGCGACCCGTGCCGCTGCCCCCGTCCCGCTCGGGAACTCACGAAAA31.9607843137254901No Hit
GGCCTGAGCGAGTCCCCGCAACTGCGGCAGTCCTTTGCCGTGGCGGGCGA31.9607843137254901No Hit
CGGCTACAGCGAGGGCGTGCTGCCGGCCGCGACCTACCCGAACCTGCAAT31.9607843137254901No Hit
GTAGGTGGTTTCCTGCGAGTGGATCGCGGCCGGGAAGACCCGCTCGCCCA31.9607843137254901No Hit
ATATGAATCAGTCGACACGTGCTTTAAAAGCTTTAGCACGTGAACCCTAT31.9607843137254901No Hit
GTCCAAGGGCCAGGCAGATTTTCCAGAAATATGGTTTTAAGCTTAAGGTA21.3071895424836601No Hit
CATTTGAAACTCTAATTGTAACGAAGAGATAATCGCAAAATTTTTCACGG21.3071895424836601No Hit
AGCCAGTACGGCTCAGTCAATCCTTGTCCATCTGCGAATGGGCGCGGGTG21.3071895424836601No Hit
GACCAGCGCCGAGCGCGACCGTCAGGAAGTGGAGGCGGCGGTCGCCTCCG21.3071895424836601No Hit
ACCTCGCCCTCCAGCGCGTGCCGGAGGAGGTCGCGATGGCGTACGGCGTG21.3071895424836601No Hit
CAGCACGATCGGCACGTCCACCGTCCAGTCGCGCGCCTGCGCCTGCGTCG21.3071895424836601No Hit
CCGTGTAAATAACGATCAGCGCGCCGGGACGGCTCTGGCGCAGAAAAGAT21.3071895424836601No Hit
GAATACGAGTGGCTGCGCGATGTGCACGATTGCCTCGAGCGCAAGGGTAT21.3071895424836601No Hit
GGCTTTCACCATGTTGGCCAGGCTGGTCTCGATCTCCTGACTTCCGGTGA21.3071895424836601No Hit
ACGTGGCCGTGGGCGCGGTGGACGCCCTCGCCGCCGTGCCGGGGGTGGAG21.3071895424836601No Hit
TAAGACTGCTTTTACCGTGTCCCACAGGTTTTGGGTTGTTGTGTTTTCAT21.3071895424836601No Hit
GCCCGCAGGAGCGCTCGGCGAGCGTGCAGAACACCCTGGAGCGGCTGCGC21.3071895424836601No Hit
TCAGCACGGCGCGGACGCCCGGGATGGCCAGGGCCGCCGCCGCGTCCACC21.3071895424836601No Hit
ATATACGGCGACGGCGGCGTCGATGGCGACGTCGATGGCGGCGGGCAAGC21.3071895424836601No Hit
GTCGTAGGCCACGCCGACCACGGTGGACTGCCACACCTTGGGGTCCGACA21.3071895424836601No Hit
AATCAAAATTAGTCATTTTTCCCTACATAGCGACAAAAGGCTGGAATGTC21.3071895424836601No Hit
GCGCCTCCGCGGGCGCGATGTCGAGGACGCGGGGGTCGATCAGACCGTCA21.3071895424836601No Hit
GGACTGGGCTTCGCGGCGCCGGAGCACGTCATCGATCACTGGGCGGAGGC21.3071895424836601No Hit
ACAATCGCTCATAGGCATCGGGGATCCGCGCGCTACGCCAGGTGTCGGAG21.3071895424836601No Hit
ATAATGAGCAGCAACGCTGGTAGAAATATTGCAATCATCCCCACTATTCC21.3071895424836601No Hit
CGCCCAGGTCACGCGCGAGATCGCGGAGCTGCTCGCCAAGCTCGCTCGCG21.3071895424836601No Hit
TCTGGGCGATCGGCCACGCGATCTCCGTTTGGGCTGCGCGCCGCGACCCT21.3071895424836601No Hit
TGATTCACTCTCGTATGTATGAAGATTGGGGATTGACCAAAATCTTTGAA21.3071895424836601No Hit
CGGCCGGATCGTCGATATGGGCGAGCGGCTGGAGCTGCACGCCATCGCCG21.3071895424836601No Hit
CGACGAAACAGGCCAGCAGGAAGATGGTCAGCAGGAACAGGAAGGGCTCC21.3071895424836601No Hit
GTCCAGCCCCGGCCGCAACGCGAGGTAGCGCGCCAGCGCGGCGCGGCGGA21.3071895424836601No Hit
CCGAACCGCTGGACGACGACACGCCGTTGCCGGCCGCGCAGGAACACGTG21.3071895424836601No Hit
GTCTTGGGGCGGGCGCTACTATTCCGACGTCGTCGCGGAGGTCGGGCCGG10.6535947712418301No Hit
AACAGATATGCTGTCTCAAAATGCATTTTTTTAAAACAACAGTTTTTATT10.6535947712418301No Hit
GACCTGCTTGTCCGGCCCGATGACCACGTGGGTGCGCACCGTCTAGATCC10.6535947712418301No Hit
GACCCGGCGCGGCTGCGCGTGATCCCGCGCGGGGTGGACGAGCGGCGCTT10.6535947712418301No Hit
CCATTACATAGGCCGGTACATCTTTCAGAATCAGTGATGCCCCACCAATC10.6535947712418301No Hit
AGGTGGGCGGATCACCTGAGGTCAGGAGTTCGAGATCAGCCTGGGCAACA10.6535947712418301No Hit
GATCTCGCCCGGCCTCGGTTCGCAGAATCGAGACGGTCACCTCGGCAAAC10.6535947712418301No Hit
GTCTTGCAAGAAGTCGAGTTGCCCATTGCCCAGCACGACGACGAGTCGCG10.6535947712418301No Hit
CTGTATATCTGATCCGTTAGTCTGAGAACTTGTTAATCTGTATATCTTAT10.6535947712418301No Hit
CGACCAGTCAGGCCGGCATAGCCACCGTCCCTCACCGAGTCATAGACCTG10.6535947712418301No Hit
ACATTGGGCTATCGTAATGCGGTGGCTGCCATTGATCCATAAGGCAAACG10.6535947712418301No Hit
ACCCAGGCCATAGTGAGTGAATGCTGGCCACAGAGCATGCCACACTAGCT10.6535947712418301No Hit
CATCAACCATATGCCGACGGATCCGATCCGCTGGCACATGGCGACGCCGC10.6535947712418301No Hit
GGTAAAGGCAAAATCGATATTCAAACTTGGGCCAATGTCCGTGGCTTATA10.6535947712418301No Hit
CACATGCACCCGCAGGCCTCGCGCGGCGGGGCGGAGATCGCCGCCTACCA10.6535947712418301No Hit
CATGATAGGCATGGCACTCGGGCAGCTTATGATCGGCCCACTCTCCGATA10.6535947712418301No Hit
GTAAAGAATGTGACCGTCCAATGCAGATTCGTACCGGAACCACAGGCGTA10.6535947712418301No Hit
CCGTTAGCTTGAAGCTTTGCTACTTCAAAAGCCTGAGCTTCTGCCAAAAT10.6535947712418301No Hit
GGCCCGGCACGGCCACGTCGAGATACTTCAGCAGGGCTTCGGCGCCGTCG10.6535947712418301No Hit
GCAACAAGTTTCGCAGTCGATCCTTTAAGTTTTGTCAGATGCTGTACTAG10.6535947712418301No Hit
AAATCAGGCAATGACCCTTAAATTTACATTGCCTGACCCCCTCTTCTTAT10.6535947712418301No Hit
GCCAAGAACTACGGGCTCCGGGAGACCACCGTGCTTTCGTTGAGCTACGG10.6535947712418301No Hit
GCCCTCGAATCAAGGGATACAGAACAGTTCTTATCCATATTAAGGATACG10.6535947712418301No Hit
GCACCGGTCATCAGTTGCGCGGCGCGCGTTTCCGGATCGGGAATCACCAC10.6535947712418301No Hit
CGCGACGGCGATGCCGGCCAGCAGTTTGATCCAGTTGCGGTTTGCGGTAG10.6535947712418301No Hit
TCAAATGACTCCTTAGAAAACTATGACTATGGCCTTTTAGAAGAAACATT10.6535947712418301No Hit
GTCCGGGTCCATGGCGCCGGCGGGCAGGATGTCCTCCTCGACCTCGACGA10.6535947712418301No Hit
AAGCTGTATATAGAAAAAGAATTAAGCTGGTTAAGCTTCAATGAACGTGT10.6535947712418301No Hit
AGACAGCACGGCGTCCACGAAGGCCGGCCCCGGCACGGCGCAGACGCGCG10.6535947712418301No Hit
CCCCATACCAGGACGGAACCAATTAGTACGGCGATGATGATGAACCACAG10.6535947712418301No Hit
CATCTCCGAGCCCACGAGACTTCGCTGGGTTTATCGCGTATGCCGTCTTC10.6535947712418301RNA PCR Primer, Index 35 (95% over 21bp)
CGCAAGGAGGAGCTGATCCAGCGCTTCGTGTACGTTCCCAGCCCCACCCC10.6535947712418301No Hit
GGCGGTGTGGACCGGGCAGCGGCGCCTTTCGATCGTCACCGCCGAGGCCG10.6535947712418301No Hit
TCTTCAGGATCATAAATTTCACGGGTATGTTCATCTAAAACTTCTGCAGG10.6535947712418301No Hit
CGAGTCAACCAGGGTGAATTGCGAGTAGGTGCCGGGACGATCGCGGTTTC10.6535947712418301No Hit
AATTTATATTTGATGATTGGCTTACTACTATTATTGATAGTGATTTTAAT10.6535947712418301No Hit
GGTCGGGCGTGAGCGTGGACGCATTGTCTTCGTACAGCTTGACCAGCGCC10.6535947712418301No Hit
GTTGTAACCAGTCACTTTGCACTTGCGTCAAGCCACTGCCAATTAGCGTG10.6535947712418301No Hit
TTCCATCGCCGCGCCCGGCCATGCCGATTCCCGCGATGCCCTCCGTGCCC10.6535947712418301No Hit
GATTAGGGTCGCTGCGATAATGAGTCCGGCTGCAAATGAGCTTGCGCCGA10.6535947712418301No Hit
GGCCATGACGGTCGCGCCGTAGGTCCAGACCAGGGCGCACTCCACCTCGC10.6535947712418301No Hit
GCACTGTGCTGGCGGCCGGTGGCGCGCTGGTCGGCGGTGAGGCCGCCGCT10.6535947712418301No Hit
CGGCAGGATCGTCCGCTCGGTGACTGCACCGTCCTTGTCGGCGTAGCGCA10.6535947712418301No Hit
TTACCACACTGTTCAAGTGCCGCTGCCTGCCGAGTCGATGTACTGGCGTG10.6535947712418301No Hit
GTAAGGACTTGCCCGGCCAGCCCGGGAGGTACGCGCTCTTCTTCGAGCGC10.6535947712418301No Hit
ACCTCCACCCGCGGATCGGTTCGGTAGTCGAGCGCCTCCGAGCGCAGCGC10.6535947712418301No Hit
GTTTATAAGCAGACATCAACCCGCAGGCTGATGTCTACACGTTCTAAAAT10.6535947712418301No Hit
GCCCCGTAAACGGCCATGGAGACCATGATCGTTGCCTTGACGTTTCCGTG10.6535947712418301No Hit
GCACGGGATCGACCTCACGGTCGAGGAGCTCGGCGTCACGGCCCTGCTCG10.6535947712418301No Hit
CCACACTATCAGTGTCGATGCGCGCGGGCTTGTTTGCCTGTCTCTTATAC10.6535947712418301No Hit
CGTTTGATGTTCTAACGAAGATTGCGAAACGCGGTCTCGGACAGTGTCTG10.6535947712418301No Hit
GAGAATGAACAGGACCAAATCCTGTTATTAGAACTGGCAAATAGCCTAAG10.6535947712418301No Hit
ATAAGTTAGTGGTCATCACCGGGTTATCTGGTTCTGGGAAGAGCTCTTTA10.6535947712418301No Hit
GATTAGGGTCTCTGCGATAATGAGTCCGGCTGCAAATGAGCTTGCGCCGA10.6535947712418301No Hit
ATATAATGTTTATCCATAATAAACGCCATTCATCTCTGAACTATAAGTTC10.6535947712418301No Hit
AGCATTGGGCGGTAATTTCTTAAAACCGGTAGTCATTACGAATGCAAATT10.6535947712418301No Hit
GAGTGGGACAAGGTCGTCAAGCTCGGGCATACCAATGGTTTCTGCAATGC10.6535947712418301No Hit
TTAGAATTAAGGGCAAAACAGATGAACTCATTCCTCTTTATATTCTGAAT10.6535947712418301No Hit
GCTGCCTCCTTGCGGTTAGCGCACTGCCTTCGGGTGAATCCAACTCCCAT10.6535947712418301No Hit
GTAGAATGCGGTGAATCCTAATTTTAATCCTTTTACATATTTTGGTAAGG10.6535947712418301No Hit
GATCTCGGGCACGAGCATGGCCGCGCCGCACGTCGCGGGCATCGCGGCCC10.6535947712418301No Hit
AGGTTCTTCATGTCCATGGATATCTGCCCGAGATTAAGTCTGTCTCTTAT10.6535947712418301No Hit
CGCCTTTGCCATACACGCTTTCGTCGGAGCTAACAATATGCAGACCGTAA10.6535947712418301No Hit
ATCGACGAGGGTATCGGCCTCGCACTGACCTTCCCCCAGGTTGGCGTGGC10.6535947712418301No Hit
CGAGATGCCATGGTCATTCTCCTTCGAGGACGTCGTGGAGATCGTAGGTC10.6535947712418301No Hit
CGCTGGGCCCCGTCGAGGAATTCGCTGGGCAGCACCTCCACGGCGGGCTT10.6535947712418301No Hit
CTTGTACACGCGCCGTATGATTAGCTTGGCAGGCCCTGCAGTCAATACGC10.6535947712418301No Hit
ACCCAGCATCCCATGCCTTTCGTGGGCCAACCCCGCGCGCCCGCGTGATG10.6535947712418301No Hit
CTTCCAGACGGCGTACCGGATGCTCGTCACGAAAGCGCGCGTGCAGGAGG10.6535947712418301No Hit
CCAAAGGAAGTAGAAACAACAGAAGATTCCTCGCGAGTTGATTATGGAAT10.6535947712418301No Hit
CTCTAGACGAACGGCAAACGCAAAGAGGAACCGGTTTTTTATGACATCGT10.6535947712418301No Hit
GGCTCGATGCGGCGCTCGACGCGCCGCGGTGGATGGTCGTGCGGCCGGCC10.6535947712418301No Hit
CCCAATGCTTGAATATCTTCAATACTACGTGATAGTTGCCCACCTTTTTC10.6535947712418301No Hit
ATCCTGAGAAGACTGTAGAAGAAGCTGAAAAGAAGAACGATAAAGGCTGT10.6535947712418301No Hit
GTTCTAACCATGGCATTGAGCACGGTCAGCAGCTTGCGCATGCAAGCGAC10.6535947712418301No Hit
ATCAAGAGGGGACCGGGAAATCGGGGTCCTACGGAATGTGGAACCACCCA10.6535947712418301No Hit
GGTAATGCCCGGAGAAGCGGCGGTCGGCGACCACGAGGTCGGGGGGGTTG10.6535947712418301No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)