FastQCFastQC Report
Tue 10 May 2022
EGAF00003626618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003626618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences826234
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41330.5002214868911228No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC17130.20732625382155662No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG16870.20417944553237943No Hit
GTACATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC15680.18977674605499167No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG15120.18299900512445627No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG13480.1631499066850311No Hit
GTACATGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG13460.16290784450894055No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT13000.1573404144588579No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG12430.1504416424402772No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT12240.148142051767417No Hit
CCCCATACCCAACCCCCTGGTCAACCTCAACCTAGGCCTCCTATTTATTC11910.14414802586192288No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT11900.14402699477387762No Hit
GTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATA11860.14354287042169653No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA11620.14063812430860992No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA11520.13942781342815716No Hit
ATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGTGTATGAGTTGG11410.13809647145965911No Hit
GGGCACTGGCCCCCAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCCC10920.13216594814544064No Hit
GGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTT10510.12720367353558434No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10350.12526717612685995No Hit
ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT10200.12345170980618081No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA10200.12345170980618081No Hit
GTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTA10120.12248346110181861No Hit
CCTTACACTATTCCTCATCACCCAACTAAAAATATTAAACACAAACTACC9690.11727912431587179No Hit
GCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAAT9630.11655293778760013No Hit
GGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACA9630.11655293778760013No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC9600.1161898445234643No Hit
GTTTAGGAGTGGGACTTCTAGGGGATTTAGCGGGGTGATGCCTGTTGGGG9500.11497953364301154No Hit
CATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGT9390.11364819167451351No Hit
GTACATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAAT9360.11328509841037769No Hit
GCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATACGAATGGGGGC9290.11243788079406075No Hit
GTATTAGGATTAGGATTGTTGTGAAGTATAGTACGGATGCTACTTGTCCA9220.11159066317774384No Hit
TCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGAC9210.11146963208969855No Hit
GGTTAATAGTGGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCAT9090.11001725903315525No Hit
TCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAAT8850.10711251292006864No Hit
GTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCC8750.1059022020396159No Hit
CATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCC8700.10529704659938953No Hit
GAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTTCG8600.10408673571893677No Hit
CCTTTAACCTCTCCGCCCTTATCACAACACAAGAACACCTCTGATTACTC8600.10408673571893677No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)