FastQCFastQC Report
Wed 11 May 2022
EGAF00003626751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003626751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3903005
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTATAGATTCTCTGGCACTCTGAGGTCTCTGTGGCAGGCCTGGTCAGG101890.2610552638287678No Hit
ACTTCAGACATCAAATCCTGCAGACAAGGGGAGCCCTCAGTCTGCAGGGC94150.24122438992519868No Hit
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGTTTGTCCCTGCGGT93300.23904658077558189No Hit
GTACATGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGG87370.22385315929649077No Hit
GACAGGATCCTCCCTGTCCCATGGCCTAAATGCCTCTCAGAACTCACAGA77930.19966666709368805No Hit
CTCCTAGCCTTGGCCTTTGGCATGAGATGGAGGCCTCCTTATTCCCCATC71670.18362774323886338No Hit
GGACGCTCTCTGGCCTTCGGCCTCCATCCTGAGGCTCCACTTGGTCTGTG63930.16379686933529422No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGTGCCACCTCTG62210.1593900084678344No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTG59880.15342024926947312No Hit
ATTGGGACTGGAGGCACCCTTAGCCACTTCATTCCTCTGATGGGCCCTGA59830.15329214284890746No Hit
ATCTCCACTGTGTCCTTAGCTCCACACAGTCAGGGCTTCCCTGAAATCTG59500.15244664047317388No Hit
GCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGA58670.1503200738917834No Hit
GTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCT58250.14924397995903158No Hit
ATGTAGTAGAGCGGCACCTCGCCACACAGGGTGTTCACGGCCATGCCCAG55820.14301800791953892No Hit
GTACATGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGG55090.14114765417927982No Hit
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCAC54810.14043025822411193No Hit
TTTCTATTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT54320.13917481530256814No Hit
GCTCCACACAGTCAGGGCTTCCCTGAAATCTGCCTCTCTCTGTCATTATG54110.13863676833619226No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA53480.1370226274370645No Hit
GGTTCTTGGCAAATGATAGGTGCTCAGTCAATAGCCTCTGTGTATAGTAA51740.13256452400137844No Hit
GTACATGGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTG49850.12772210130399525No Hit
TCTATACTGTGTCCTTAGCTCCACACAGTCAGGGCTTCCCTGAAATCTGC49390.12654352223479087No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG49050.1256723985749442No Hit
GCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGG48700.12477565363098433No Hit
GTGCAGAGCTAAGGACACAGTGGAGATTCTCTGGCACTCTGAGGTCTCTG48670.1246987897786449No Hit
GGACGCTCTCTGGCCTTCAGTTTCCATCCCCAGGATCCACTTGGTCTGTG45490.11655122143066689No Hit
CAGTGGAGATTCTCTGGCACTCTGAGGTCTCTGTGGCAGGCCTGGTCAGG44760.11468086769040778No Hit
CTTCAGTTTCCATCCCCAGGATCCACTTGGTCTGTGAGATGCTAGAACTC42760.10955661086778008No Hit
GTCACAGGGTTGGCTTGGGCACCACCGTGCTGGGGCACCACTGGGCCCTC41500.10632832906952465No Hit
GAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAG40900.10479105202273634No Hit
GTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGTTCACGGCCA39590.10143466380391518No Hit
CCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGTTTG39070.100102357030032No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)