FastQCFastQC Report
Wed 11 May 2022
EGAF00003626752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003626752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3903005
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTATAGATTCTCTGGCACTCTGAGGTCTCTGTGGCAGGCCTGGTCAGG98160.2514985248545672No Hit
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGTTTGTCCCTGCGGT93120.2385853976615454No Hit
GTACATGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGG88590.22697895595829368No Hit
ACTTCAGACATCAAATCCTGCAGACAAGGGGAGCCCTCAGTCTGCAGGGC76450.19587471704494355No Hit
CTCCTAGCCTTGGCCTTTGGCATGAGATGGAGGCCTCCTTATTCCCCATC69920.17914401851906414No Hit
GACAGGATCCTCCCTGTCCCATGGCCTAAATGCCTCTCAGAACTCACAGA67320.17248248464964816No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGTGCCACCTCTG62580.16033799598002052No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTG60600.1552649817256191No Hit
GGACGCTCTCTGGCCTTCGGCCTCCATCCTGAGGCTCCACTTGGTCTGTG58360.1495258140842761No Hit
TTTCTATTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT56400.14450404239810094No Hit
ATGTAGTAGAGCGGCACCTCGCCACACAGGGTGTTCACGGCCATGCCCAG55360.14183942885033454No Hit
ATTGGGACTGGAGGCACCCTTAGCCACTTCATTCCTCTGATGGGCCCTGA53740.13768878082400612No Hit
GCTCCACACAGTCAGGGCTTCCCTGAAATCTGCCTCTCTCTGTCATTATG53260.13645895918657547No Hit
GTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCT52900.13553659295850248No Hit
GTACATGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGG52230.13381996692292222No Hit
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCAC50190.12859322496384196No Hit
GTGCAGAGCTAAGGACACAGTGGAGATTCTCTGGCACTCTGAGGTCTCTG49170.12597985398430184No Hit
GGTTCTTGGCAAATGATAGGTGCTCAGTCAATAGCCTCTGTGTATAGTAA49160.12595423270018868No Hit
ATCTCCACTGTGTCCTTAGCTCCACACAGTCAGGGCTTCCCTGAAATCTG48860.12518559417679453No Hit
GTACATGGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTG48500.12426322794872155No Hit
GCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGG48430.12408387895992959No Hit
TCTATACTGTGTCCTTAGCTCCACACAGTCAGGGCTTCCCTGAAATCTGC46630.11947204781956468No Hit
GCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGA46480.11908772855786759No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG46320.11867778801205739No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA45680.1170380258288165No Hit
CAGTGGAGATTCTCTGGCACTCTGAGGTCTCTGTGGCAGGCCTGGTCAGG43540.11155507102860489No Hit
GTCACAGGGTTGGCTTGGGCACCACCGTGCTGGGGCACCACTGGGCCCTC42770.10958223215189321No Hit
GGACGCTCTCTGGCCTTCAGTTTCCATCCCCAGGATCCACTTGGTCTGTG42590.10912104903785672No Hit
GAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAG41710.10686637603590055No Hit
GTTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGC39520.10125531481512322No Hit
TCCTAAGACCTGGCACATGTTAATTTTAATGATTATCTGGGTGAATGTAT39360.10084537426931299No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)