FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003626809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003626809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTTATGGTGAAGCAGGCAAGCTTATGCCAGGCTGCAGGGTCAGAGACA33.296703296703297No Hit
CTCTAGGATCACCCCTCCATCTACAATTTCATCCACAGCCAAGAACAGCC22.197802197802198No Hit
ACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACT22.197802197802198No Hit
GTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGT22.197802197802198No Hit
CGCTCGCGCAGCGCCGCCGTGCGGCGGAAGGTCAGCGACTGAAGCAGGCC22.197802197802198No Hit
GTGGTGGCCGAGTCTGAGCTACAGAAGGAACTAAAGAAGGTGAAGACAGC22.197802197802198No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22.197802197802198No Hit
CTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCATC11.098901098901099No Hit
GTATTCATTTTCTTCCTTGTACAAAGTGATGACTTCTAATGCTTATATCT11.098901098901099No Hit
GAATCGAAGAGAATCTTCGAACGGACCCGAATGGAATCATCTAATGGAAT11.098901098901099No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.098901098901099No Hit
GGAGATGGCGGAAATGGATCCGGTAGCCGAGTTCCCCCAGCCTCCCGGTG11.098901098901099No Hit
GTAATCATCATCGAATGGAATCGAATGGAATCATCGAATGGACTCGAATG11.098901098901099No Hit
ACTTATTTGTGGTGTGTGTATTCAACTCACAGAGTTGAACCTTCCTTTAG11.098901098901099No Hit
GGGAAAGGAAAAAGCCAGGAGACTAAAGGGGGAAAAAAAACTCAAAAAAG11.098901098901099No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
ATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAA11.098901098901099No Hit
ATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAA11.098901098901099No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA11.098901098901099Clontech Universal Primer Mix Long (96% over 26bp)
CGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC11.098901098901099No Hit
GTATATAGGCTGAGCAAGAGGTGGTAAGGTTGATCGGGGTTTATCGATTA11.098901098901099No Hit
TCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTC11.098901098901099No Hit
GAGATGTACTTCTCAGTGGCAGTATTGAACTGCCTTTATCTGTAAATTTT11.098901098901099No Hit
CCCTTTGGCTCAGCCCGCGCGCCCCAGGCCCGGCCCGGGCGGCGCGACGG11.098901098901099No Hit
CGGTGCCCGAGCCCACGTTGCCCAGCACTGGGATCTTCTTCTGGTTGGCG11.098901098901099No Hit
ACTTATTCGAGTTGTCCACAGTCAGCAATGGTGATCTTCTTGCTGGTCTT11.098901098901099No Hit
AGGCTGGACTGCAGTGGCGCGATCTCGCGTTTCAGTTATATATTAAGCCG11.098901098901099No Hit
AGGCTGGACTGCAGTGGCGCGATCTCGCGTTTCAGTTATATATTAAGCCT11.098901098901099No Hit
CTGTTGCTCATGCCTGTAATCGCAACACTTTGGGAGGCCATGGTGGGAGG11.098901098901099No Hit
AATTAGTGACGCGCATGAATGGATGAACGAGATTCCCACTGTCCCTACCT11.098901098901099No Hit
GTCTTTCCCTGTTGGTGATCACACCAACACGACCGAGGTTGGCTCCACCA11.098901098901099No Hit
ATGTTATTAAAAGCAACTATTATTGCTTATTGTAACAATGATCCCTGATT11.098901098901099No Hit
CCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGGTTTTAAGCAG11.098901098901099No Hit
TTACATGGTATGTTAATCATATCTTAATGAGACTCAGCCCCTCCCCCAGA11.098901098901099No Hit
GGGTCTCTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCT11.098901098901099No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC11.098901098901099No Hit
AAAGTGACGACATTATTTCAACAGCCACAGGGAAAATTGAGAAAATAATC11.098901098901099No Hit
TAGTAAGATAGTAGAGTTTATTTTGCCCAGTACATATTAAAAACTGTCAA11.098901098901099No Hit
GTGTTAGAGATAGTAATAGGAGCAATTTGCTCACCTGCTTTTGATAACAT11.098901098901099No Hit
ATCCAAACATGCTCCAAGTTCAGGAAAGAGGACACCTGGGCTCAACTTAT11.098901098901099No Hit
ATCATGCAATGCTGAGCACTGGGGTGGTGAGCTTCTTCTCCCTCAAGTCG11.098901098901099No Hit
ATGTTATTATAAGCAACTATTATTGCTTATTGTAACAATGATCCCTGATT11.098901098901099No Hit
CTGTAATTAGCATTTAGAAGCTGAAGTTGGGGATCTTCAGCTTCTAAATG11.098901098901099No Hit
CTTCAACTGCCAAAAGCACAGCCCTTTTTCTCTGAGCTGGTGGTTCTGGC11.098901098901099No Hit
CGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACA11.098901098901099No Hit
AAGAAAAACACAACAGAAGAGATGGAAAAAAACATGGAGGATGCAGTGTG11.098901098901099No Hit
GCTATGAACGCTTGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTC11.098901098901099No Hit
CATAGAGGGCTCCCAGAAGTGTGTGGCTGAGCTGGGTCCCCAGGCCGTGG11.098901098901099No Hit
GCCCCATTCCTCTGATTCCCAGTGGCTGCTAGCTGGACCCAGCTGGTGTC11.098901098901099No Hit
CTCATGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCC11.098901098901099No Hit
AATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGGTT11.098901098901099No Hit
GTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGG11.098901098901099No Hit
GATGAATGCACCGATGGCCACGCCGATGATCATCGGAAGGAGCATGGGGC11.098901098901099No Hit
ACTACAGACATCTCTGTGCTGTTTATTACAACAAGAATCCTGGGTTTGAG11.098901098901099No Hit
CTCTCCTACTCCTGCTCGCATCTGCTATAGTGGAGGCCGGAGCAGGAACA11.098901098901099No Hit
CCTTTGCTGTGACTGGCTATGACCTTGCGGAGATCCGGAGATTCAGCAGT11.098901098901099No Hit
CTCCACCTCCCTGGTTCCAGCAATTTTCCTGCCTCAGCCTCCCAAGTAGC11.098901098901099No Hit
CCCGGGTTTATGTCCATTTCCTCCGCCGCTCCCACCGTGGTTGCCGGAGG11.098901098901099No Hit
CATACAGGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCACGGT11.098901098901099No Hit
ACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACC11.098901098901099No Hit
ATCGATACACCAAGTAATACATGTAACAAGTTCTTGAATTCTATCATCTA11.098901098901099No Hit
CAAGCACACCCTGGGAGACAGTGATAATGAAAGCTAAGCCTCGGGCTAAT11.098901098901099No Hit
TCTTCACTCACGCCGAGCGCACGCCCGAACTGACGGTGTTCTCCCGCCCC11.098901098901099No Hit
GCACAAAAGGGACCCGCTGCAGACTCAGGGCAAAGGGAATGCCATCGGTG11.098901098901099No Hit
GTGCCAGGAGTGTGGAAAATCCTTCCGGCAAAAAGGTAGTCTAACGTTAC11.098901098901099No Hit
GACGTCACACGAAGGGACTCATAGCTGTGGTCTGGGGCTTCACCGAGGGC11.098901098901099No Hit
GCTTAATTTGACACAACACGGGAAACCTCACCCGGCCCGGACACGGACAG11.098901098901099No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA11.098901098901099No Hit
CCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCAGTCAAAC11.098901098901099No Hit
GCTTGTGAAGGGCTGTCTTAGCCTTCTCGGCAACATGTTCAATGGCTGAC11.098901098901099No Hit
CAATTGAAGTGAAGATGATTGACCGCACGCAATTGATATTAGATATTTTT11.098901098901099No Hit
GCATGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGA11.098901098901099No Hit
GTTATTATCTGTGGTTTTAGACATCCACTGGGGGTCTGGTTTTAGATATC11.098901098901099No Hit
GTACGTACAGCATTGGCAGTGGTGCGTCAGAGGTGGCAGAACTATTTCAC11.098901098901099No Hit
GGGGTTTTCAGCCCTCGCGGGTGGGCAGCGTCTTGTCCTCAGGTGTAGAT11.098901098901099No Hit
GACTGAGGCAGAGAATCACTTGAACCCGGGAGGCAGAGGTTGCAATGAGC11.098901098901099No Hit
GTTCGAGACCAGCCTCGCCAACATGGTGAAACCCCATTTCTACTAAAAAT11.098901098901099No Hit
GCCGAGGGGGTTGGCCAGGGTCCCGGCCCCGGCCTCCGCCGCCGACTCCA11.098901098901099No Hit
GTGCATGCAGTCCGGGCAAACAAGCTTCAGGGGCTGGCAGTCCTTGAATC11.098901098901099No Hit
TCAGAATCTTGAGGAGCAAGGCAGCTATTAATTAGAAAAGGTATCTCCAC11.098901098901099No Hit
TCATCGAGAGGTGCCCCAAGCCCGTGATTGCTGCCGTCCATGGGGGCTGC11.098901098901099No Hit
GCCCAGCTAAGCCAGACCAGCCTCCCGGCCGCCCGGCGCCCTGGGCTCGC11.098901098901099No Hit
ATCATAGCTCACTGCAGTCTGCCTGCCAGGTTCAACACATTCTCATGCTT11.098901098901099No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)