FastQCFastQC Report
Tue 10 May 2022
EGAF00003626954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003626954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150785
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12600.8356268859634579No Hit
GGAACAGGTTGAACAGTCTACCCTCCCTTAGCAGGGAACTACTCCCACCC7030.46622674669231023No Hit
GGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGC3090.20492754584341943No Hit
GTACATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAAT3020.20028517425473355No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2800.18569486354743509No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT2690.17839970819378584No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG2470.1638093974864874No Hit
GTACATGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG2360.15651424213283816No Hit
GGGCACTGGCCCCCAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCCC2270.1505454786616706No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG2110.13993434360181717No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1980.13131279636568624Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT1940.12866001260072288No Hit
ATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCATTATCC1910.12667042477700038No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC1900.12600722883575952No Hit
TATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATATCATAG1900.12600722883575952No Hit
GTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGCCACCTACGGTGA1900.12600722883575952No Hit
GAATATGATAGTGAAATGGATTTTGGCGTAGGTTTGGTCTAGGGTGTAGC1880.12468083695327783No Hit
ATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCC1870.12401764101203701No Hit
GGGTTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGACGTAGA1860.12335444507079617No Hit
GGCCTATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCAT1780.11804887754086944No Hit
CACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCAC1760.11672248565838778No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA1750.11605928971714694No Hit
GCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTGGT1750.11605928971714694No Hit
GGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTG1750.11605928971714694No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1720.11406970189342441No Hit
GTATTAGGATTAGGATTGTTGTGAAGTATAGTACGGATGCTACTTGTCCA1700.11274331001094275No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC1680.11141691812846105No Hit
GCCTTCATAGGCTATGTCCTCCCGTGAGGCCAAATATCATTCTGAGGGGC1660.11009052624597938No Hit
GTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTT1660.11009052624597938No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG1640.10876413436349769No Hit
GTACATGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTA1610.10677454653977517No Hit
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT1590.1054481546572935No Hit
GGGTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAAT1580.10478495871605266No Hit
GTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATA1550.10279537089233014No Hit
ACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATAC1510.10014258712736678No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)