FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003627107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003627107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTCCTCCTCCCTTCTGGTCAGTTGGATTTGTCACTGTTCAGCATCTTT42.3391812865497075No Hit
CCCGACCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATT31.7543859649122806No Hit
CTCCCAAGATCCAACTACGAGCTTTTTAACTGCAGCAACTTTAATATACG31.7543859649122806No Hit
TGGGTGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCC31.7543859649122806No Hit
ATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCACCTGC31.7543859649122806No Hit
CCCCAAGTTAGTTCAATCTGGATTCATATTTAATTTGAAGGTAGAATGTT21.1695906432748537No Hit
GACTACTCCGCAGCTCCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAGT21.1695906432748537No Hit
GGGCGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGC21.1695906432748537No Hit
TAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAGGAA21.1695906432748537No Hit
CTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGT21.1695906432748537No Hit
GCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCTGAGTGAGCACCTG21.1695906432748537No Hit
GGAAACGCCCCGGGCAAAGGGTCTTTTGCAGCTTTTGCAGACGGGCAAGA21.1695906432748537No Hit
ATTCCAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCAC21.1695906432748537No Hit
GTCCTCCGGTGCTCCATGGATGACAGCGCTGGCCCAAGGTCGCCGACAGG21.1695906432748537No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACAC21.1695906432748537No Hit
CCACTGGGGTCAGTCACTCGAAGCACAATAAATATAAAATGTGGTCCTTC21.1695906432748537No Hit
CCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGTGT21.1695906432748537No Hit
GGCCCCTGGGCGCCACCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCA21.1695906432748537No Hit
GTGTGGCGAGGTGCCGCTCTACTACATCTAGGACGCCTCCGGTGAGCAGG21.1695906432748537No Hit
CTCAGCACCCTCCGGAGGGGACCCGGCCTTCCTGGGCATGGCCGTGAACA21.1695906432748537No Hit
GTATCAACGCAGAGTACATGGGAGGAGAGGAGAGCATAGCACCTGCAGCA21.1695906432748537No Hit
GCCCAGGCTGGAGTACAGTGGCTCGGTCACAGCTCACTGCTTCCTGCAAC21.1695906432748537No Hit
GCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCAC21.1695906432748537No Hit
TCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGA21.1695906432748537No Hit
CTGTGTGCACAGCAGCTGTGCCTCCGTAAATGGTTTTACAAAGCCTGCTG10.5847953216374269No Hit
CCTGAGGGGTGGCTGTTAATTCTTCAGTCATGGCATTCTGTCTCTTATAC10.5847953216374269No Hit
CCACGACAGCGGCCTGGACTCCATGAAAGACGAGGAGTACGAGCAGATGG10.5847953216374269No Hit
AAGTTGTACCACTCAGAAGCCTTCACTGTCAACTTCGGGGACACCGAAGA10.5847953216374269No Hit
GCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCG10.5847953216374269No Hit
CCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGT10.5847953216374269No Hit
CCGGTGAGCAGGGTCAGTGGAAGCCCCAACGGGAAAGGAAACGCCCCGGG10.5847953216374269No Hit
CGCTCTTCCCGGTCCGGCTCCTGGTTGCCGCTGCCATGATGCTGCTGGCC10.5847953216374269No Hit
AGCCAGCCCCTGGCACCTGCTGCTACATCATGAAGATAGCTCCAGAGAGC10.5847953216374269No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.5847953216374269No Hit
GAGACGAGTCAAGGCGGCGAAGTGGTTGTTCGGGATGTCGACCGCGTTGG10.5847953216374269No Hit
GAAACACACGGAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCC10.5847953216374269No Hit
CACCCAGCTCTTCTGGCTCCAAAACCCAAAGTTTAGCTTCCCAGTCCCCG10.5847953216374269No Hit
TGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTCATGGGT10.5847953216374269No Hit
AGCTGGGGCAGCATGCGGCCCAGCAGCGTGTCGAGCAGGATGACCTGTCT10.5847953216374269No Hit
CCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGGGGCCGAGTTG10.5847953216374269No Hit
CAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATA10.5847953216374269No Hit
GATGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATTGAGAG10.5847953216374269No Hit
GCAGTGAGCCGAGATCACGCCACTGCGCTGCAGCCTGGGTGATACCTGTC10.5847953216374269No Hit
CACCCAGCTCTTCTGGCTCCAAAACCCAAAGTTTAGCTTCCCAGTCCCCT10.5847953216374269No Hit
CGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAG10.5847953216374269No Hit
CGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGG10.5847953216374269No Hit
GTCCCACACACCCCGAGGGCCTGGCAGGTGGTGTGGGCATCCCAGCCCCT10.5847953216374269No Hit
GGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACT10.5847953216374269No Hit
GTCTGTGGCCTCTCCTTCACCAGACGCAGGAGTTTTGGCCTCTGCAAGGC10.5847953216374269No Hit
TCGCCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCC10.5847953216374269No Hit
CCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAGAAAAGTCCACAACTT10.5847953216374269No Hit
GGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCCTGTC10.5847953216374269No Hit
GGGCCTGGCTCGCGGCGAACCGAGATGGCAGAGCAGTCGGACCTGTCTCT10.5847953216374269No Hit
CCACAATCAAGTTCCAAATGAAGAAGGTGTTATGTCTGGCTGTAGCCTGT10.5847953216374269No Hit
GGTGTGGATGGCCGGGATGGTGTTAATGGTTCGGCTGGGCTGGTTGGCCC10.5847953216374269No Hit
CCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGC10.5847953216374269No Hit
CTGGTATCCCTGGAGAGTGTGGAGAGAAGGGGGAGCCTGGCGAGAGGGGC10.5847953216374269No Hit
CCCACAATCACCACGACGATGAGGACCACCACCACCACCACGATAAGAAG10.5847953216374269No Hit
GGCTTGACACTGCCAGGCGCCTATTGCTTGACCTCTGTTTAAATGAGGGA10.5847953216374269No Hit
CTCCCCCACAACTGGGGTGCCTGGGGGGAACTTGGTCTGCTTCAGCCCAA10.5847953216374269No Hit
GGTATCAACGCAGAGTACATGGGAGGAGAGGAGAGCATAGCACCTGCAGC10.5847953216374269No Hit
GGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCT10.5847953216374269No Hit
CTCCTGATGCGTGAGCGAGAGTATCGCCTTCCTCTGGATCGAGAATGTGA10.5847953216374269No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC10.5847953216374269No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA10.5847953216374269No Hit
ATGGTGACCGCTACGACTTCCCCCTGCTCCAAGCAGAGCTGGCTATGCTG10.5847953216374269No Hit
CCCTCATGTGAATCCACTTCCAACTGGCTACGAAGATGAATAAAGAGAAT10.5847953216374269No Hit
CCCCAACTCTGATCAGGGACTCCCTGTGCTGATGACTCCTGTCTCTTATA10.5847953216374269No Hit
CTCTAGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGT10.5847953216374269No Hit
AGAAAGAGCTATCAATCTGTCAATCCTGTCCGTGTCCGGGCCGGGTGAGG10.5847953216374269No Hit
CTGCTGGGCCGCATGCTGCCCCAGCTGGTCTGCCGCCTCGTCCTCCGGTG10.5847953216374269No Hit
ACTCAGGGCCAGGCGTTGCTGGGCTTCCGGCGCACCAATGCTCATCTCCA10.5847953216374269No Hit
GTGGGAGACCACGTTTTATTCAGTCCAGTTCAGGATCCCCGCTATCTCAG10.5847953216374269No Hit
CTAGAAAAGTCCACAACTTCCAGATGGAATGCTCTCTGCAGGCCAATCCC10.5847953216374269No Hit
GTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTGGT10.5847953216374269No Hit
GGGTAGTCGTGAACACCAGTGGGTTCTGCCCTGGGCAGCTCCCCACCTTC10.5847953216374269No Hit
GCAAAGAGGTCCTGATGGAGAGCCCGCCGGACCTGTCTCTTATACACATC10.5847953216374269No Hit
CGCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGC10.5847953216374269No Hit
GGTTTCCCGTGTTGTGTCAAATTAAGCCACAGGCTCCACTCCTGGTGGTG10.5847953216374269No Hit
TTCCCATCTCGTCCATGAGCCTAGGTCTTGGAGCCTTGTGTTGGAGGCTG10.5847953216374269No Hit
CCTTTTTTTTGTTTGTTTTAGTTCTATTTATTTCTGCTTTGATTTTTGTT10.5847953216374269No Hit
GCCCAGCTTAGACGTAGGCACTGCGGGCTTGGCCTGCAGAGAGCATTCCA10.5847953216374269No Hit
CTTCATGTCCCCGCGGTCGAAGACGGCCACATACGTCCCCAAGAAGACGT10.5847953216374269No Hit
AAACGCCCCGGGCAAAGGGTCTTTTGCAGCTTTTGCAGACGGGCAAGAAG10.5847953216374269No Hit
CAGCACCCTCCGGAGGGGACCCGGCCTTCCTGGGCATGGCCGTGAGCACC10.5847953216374269No Hit
AGATGGATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTAC10.5847953216374269No Hit
CTGGTATCCCTGGAGAGTGTGGAGAGAAGGGGGAGCCTGGAGAGAGGGGC10.5847953216374269No Hit
GAAACGCCCCGGGCAAAGGGTCTTTTGCAGCTTTTGCAGACGGGCAAGAA10.5847953216374269No Hit
GTGACATTGTGTGAAGATTGAATAGTACATAATATGCTGTATTAGAAGTG10.5847953216374269No Hit
GGGCACAAGAGGGGAGTAGGTAACAAGATAAAAGGAGCTCCCTCCCTGAT10.5847953216374269No Hit
GCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGAC10.5847953216374269No Hit
CCACTCCTGAGCTGAATAAAGATGATTTAAGCATAATAACTGTCTCTTAT10.5847953216374269No Hit
GTGCATACAGATGCCGTTTGAGTCAGTCTGGTTCTGGAAGTAGTCGATGA10.5847953216374269No Hit
CGTCCGCCACGAGCATCGCCACCCGGCGCGTCCTGATGCTGCCGTCACCC10.5847953216374269No Hit
GGGTGCTGATGTCAATGCTCAGGAGCCCTGTAATGGCCGGACTGCCCTTC10.5847953216374269No Hit
GCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTC10.5847953216374269No Hit
CTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCTGTCTCTTATACAC10.5847953216374269No Hit
ATAGAGACAAGCTTGGAGAAAGAGATGACACTATCCCACCTGAATACAGA10.5847953216374269No Hit
GCCCTGGAAGCAGCAGCTCACCGAGGACGGGGACTCGTTCCTGCACTTGG10.5847953216374269No Hit
AGTCCCAGCTACTCAGGAGTCTGAGGCAGAAGATTGCTTGAACCCAGGAG10.5847953216374269No Hit
CGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGG10.5847953216374269No Hit
GCGAGCGACCAAAGGAACCATAACTGATTTAATGAGCCTGTCTCTTATAC10.5847953216374269No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG10.5847953216374269No Hit
GTACATGGGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACG10.5847953216374269No Hit
ACACACGGAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGC10.5847953216374269No Hit
GTAGTCCGGCGGGCTCTCCATCAGGACCTCTTTGCTGCCCACATCCTGTC10.5847953216374269No Hit
CGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCC10.5847953216374269No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCTGTC10.5847953216374269No Hit
GCCTCCGAGGCTGCCATGTACTCCGCTTCACATGTAGATCCCGCCACGAC10.5847953216374269No Hit
GTAACTGCTTTTCCCTGTAAAGTTGGGCCTAATCGGAACTGTAGCTCATA10.5847953216374269No Hit
CCTTGGCGCCTGCCTGATCCTCCAAATCACCACAGGACCTGTCTCTTATA10.5847953216374269No Hit
AGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCC10.5847953216374269No Hit
TCCCAAGGGTGCGCTAGCTGTGGCAGTGGCCCAGGTGTGCCGCGTGGTAC10.5847953216374269No Hit
GGACACTGCCAGTCACTCTGGCACTCAGGTTTCTTGTATCTAAGGCACTG10.5847953216374269No Hit
CTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGC10.5847953216374269No Hit
TGTTAATTTTTGATGCCATGCACTCATTTCCAGCCTGTCTCTTATACACA10.5847953216374269No Hit
AGGTGGCGCCCAGGGGCCCGGGAACTCCTGCCACAACAGAATAAAGCCTG10.5847953216374269No Hit
CATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGG10.5847953216374269No Hit
CTTTCGTTCTTGATTAATGAAATGTTTTCATTAATCAAGAACGAAAGTCG10.5847953216374269No Hit
CCACGGGGGGCGTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCG10.5847953216374269No Hit
GTCGTCAGAACACATCAACGAGGGGGAGACGGCCATGCTGGTCTGCAAGT10.5847953216374269No Hit
ATTTGGGGGTGGGCAGGCAGCGACCCACTACCTTGTACTCTCGTAGCGTG10.5847953216374269No Hit
CCCCTGTTGCCTGAAGCATCTTCAAATGATCCACTGTCATTTCTGTCTCT10.5847953216374269No Hit
GCAGGTGTGTATGTGAACATTTCCATAAGCACATAAGGATACAGATGTCA10.5847953216374269No Hit
TCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCAT10.5847953216374269No Hit
TCTTATACACATCTCCGAGCCCACGAGACTATGTCTGCCTAATCTCGTAT10.5847953216374269No Hit
GCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGG10.5847953216374269No Hit
GAGGGTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAGACGT10.5847953216374269No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC10.5847953216374269No Hit
CAGCACCGTGGAGTGGGCGCGCCACGCGCCCTTCTTCCCCGAGCTGCCGG10.5847953216374269No Hit
TTGTTGCAGGGCATGGAGGCTCACACCTGTAATCCCAGCACTTTGGGACG10.5847953216374269No Hit
GTAACACTGTGTATCAGATGACCTTAGCCATAGAGTTGGTCTTTAGAGCT10.5847953216374269No Hit
TCCTGTCCGTGTACGGGCCGGGTGAGGTTTCCCGTGTTCTGTCTATTATA10.5847953216374269No Hit
CCTCTGAACCGAGTCCTGGCCAACCTGTTCCTGCTCATCTCCTCCATCCT10.5847953216374269No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT10.5847953216374269No Hit
GTCGTGGTGATTGTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAA10.5847953216374269No Hit
GGACATCCACACCGAAAACCCATCTGTACATCACCATCATCAAAGACCAA10.5847953216374269No Hit
GAGAGAGAGCAATGAGGGCATAGGTGGCTGATTACATCAGATGGGTTAAT10.5847953216374269No Hit
ACGCAGAGTACATGGGGGAGGAGAGGAGAGCATAGCACCTGTCTCTTATA10.5847953216374269No Hit
CCTCAAATTCCTGGGCTTGACCAGGCTCAGTGGCTCACACCTATAATCCC10.5847953216374269No Hit
AGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGAC10.5847953216374269No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)