FastQCFastQC Report
Sat 23 Apr 2022
EGAF00003627112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003627112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1224
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12810.457516339869281No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1129.15032679738562No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG544.411764705882353No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC393.1862745098039214No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC383.104575163398693No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG352.8594771241830066No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC322.6143790849673203No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG282.287581699346405No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA231.8790849673202614No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC221.7973856209150325No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT201.6339869281045754No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA191.5522875816993464No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG171.3888888888888888No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA161.3071895424836601No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC141.1437908496732025No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG131.0620915032679739No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC120.9803921568627451No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT110.8986928104575163No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG110.8986928104575163No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT100.8169934640522877No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC100.8169934640522877No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA100.8169934640522877No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG90.7352941176470588No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC90.7352941176470588No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC90.7352941176470588No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT90.7352941176470588No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC90.7352941176470588No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG90.7352941176470588No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT90.7352941176470588No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA80.6535947712418301No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG70.5718954248366013No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA70.5718954248366013No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA60.49019607843137253No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT60.49019607843137253No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG60.49019607843137253No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA60.49019607843137253No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG50.40849673202614384No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT50.40849673202614384No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA40.32679738562091504No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA40.32679738562091504No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC40.32679738562091504No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA40.32679738562091504No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA40.32679738562091504No Hit
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT30.24509803921568626No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG30.24509803921568626No Hit
AGATCCGCTGGCAGATCGACGAGATCGAGGACGGCCACAAGATCGAGCAG30.24509803921568626No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA30.24509803921568626No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA30.24509803921568626No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGAT30.24509803921568626No Hit
TATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGG30.24509803921568626No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA30.24509803921568626No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC30.24509803921568626No Hit
CCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGG30.24509803921568626No Hit
GAGTGGGAAAAAGACCTCAAAGCCATTTCAGAGACCTTCATGGCAGCACC30.24509803921568626No Hit
CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA20.16339869281045752No Hit
ACATCCAACAGCGGTTGAAAGTGCTGCGGCAGGTGGAGGCTGAACGGTCT20.16339869281045752No Hit
GCTCGGGCCTGCCCTGCACCCCCCGCGCCCCCGGCCAGGCTAGGCGTCCT20.16339869281045752No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA20.16339869281045752No Hit
GGAGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG20.16339869281045752No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTA20.16339869281045752No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC20.16339869281045752No Hit
GCTCGACTCCGAGGTCGACGCGGCCCTGGAACGAGCCCCAGGCACCATGA20.16339869281045752No Hit
GCTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTG20.16339869281045752No Hit
TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT20.16339869281045752No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGT20.16339869281045752No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCA20.16339869281045752No Hit
GTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATG20.16339869281045752No Hit
GTACATGGGTAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT20.16339869281045752No Hit
ACTCTGCGTTGATACCACTGCTTACCCATGTACTCTGCGTTGATACCACT20.16339869281045752No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA20.16339869281045752No Hit
GCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG20.16339869281045752No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA20.16339869281045752No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTG20.16339869281045752No Hit
GTGCAGGGCGGTCCGCCTCTCAGACGTAGAGGCCCGGCCTCGGATGAAGG20.16339869281045752No Hit
TTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAT20.16339869281045752No Hit
GCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTG20.16339869281045752No Hit
ATTTAACACATTCTGTACAAGGTCTTCACTTTGCTGTCATCATTTGTACA20.16339869281045752No Hit
GTTTAGTAGTTTCATCATGAAAAGGTATTACATTTTGTCAAATGCCTTCC20.16339869281045752No Hit
ACTCATCACTGTGAGAACAGGCAATCAGGAAAGCTAATTTCTTAATTAGC20.16339869281045752No Hit
GCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGATACCACTGCTTCC20.16339869281045752No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG20.16339869281045752No Hit
GTATCAACGCAGAGTACTTGGAAGCAGTGGTATCAACGCAGAGTACATGG20.16339869281045752No Hit
GAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAG20.16339869281045752No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGGGGTA20.16339869281045752No Hit
ATGGAAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA20.16339869281045752No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTTCCATGTACTCTGCGTTGAT20.16339869281045752No Hit
GTGGTATCAACGCGGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC20.16339869281045752No Hit
GCGTGTAGGTCATCCTTCGCCGAATGCTCAAGGGCCGCAAGTCGCTGAGC20.16339869281045752No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG20.16339869281045752No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT20.16339869281045752No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGG20.16339869281045752No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT20.16339869281045752No Hit
CTAGGACACTTTAAAAAAGATTGTCCAGTGAGAAACAAGCCACCCCTTTG20.16339869281045752No Hit
GTACTTGGAAGCAGTGGTATCAACGCAGAGTACTTGGAAGCAGTGGTATC20.16339869281045752No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT20.16339869281045752No Hit
CCCACTAATAGGGAACGTGAGCTGGGGTTAGACCGTCGTGAGACAGGTTA20.16339869281045752No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA20.16339869281045752No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACTC1850.001416276315.2432435
GTACTCT1900.001695611414.8421066
TACTCTG1900.001695611414.8421067
CTCTGCG1950.002020217514.4615389
ACTCTGC1950.002020217514.4615388
ATGTACT2100.003326594613.4285724
CATGTAC2150.003896148413.1162793
CCATGTA2200.0045459612.8181822
CCCATGT2200.0045459612.8181821
Produced by FastQC (version 0.11.5)