FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003627191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003627191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52
Sequences flagged as poor quality0
Sequence length100
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCAACTGGGACAGTTTTTAATCCGCCCTTTTCCCGACGAGAATTGTT35.769230769230769No Hit
CGTCTACCTTCCCTCCGACGCTCTTCCGATCTCTGGTACCGACAGAATCT23.8461538461538463No Hit
TACTCACCCATTGTTTGATGACGGCCGCGACGGCGCGCGGGTTGCTCTGC23.8461538461538463No Hit
GCAGGAGATCATCCCGGAGGGCGATTTCGTCGGGTCCATAGACGCAGTGT23.8461538461538463No Hit
GTTCCACCGTGCTTCGGACCTTGCCCTTCTGCATGCACGGCCTTGCTGCC23.8461538461538463No Hit
GGCGCGCTGCGTGGCGTCCTGTTCCGCGCCAAGAAGGATGGCGTGAACTA23.8461538461538463No Hit
TCGCTTTATCGGTCGACGTTGTGCACGAAACCTACGAGGACGGCGTTGAG23.8461538461538463No Hit
GTGTCGGTAGCGGGAAATGCCTTTCCGTTGATGGTGAAGTAGTTCGGCAA11.9230769230769231No Hit
CACGTAAGGCTTGGACTTGTAGCCTTCCTTCTCGAACGCACGGCGCATCT11.9230769230769231No Hit
GGAAGAACCGTGCGCACAGTTGATAACTATATCGCCCTGCTCTGTTCGCT11.9230769230769231No Hit
TGTAGCGCGGGTCCACCACGATGACCTTGCAGCCCGTTTCCTTGGCATGC11.9230769230769231No Hit
GCCCTCGCCTGCATGTCGTTGGCGTCGTTCTGGGCTTTCATCGAGGCCAT11.9230769230769231No Hit
CGCCGGAACTGGAGCGGCCCTCCGGCAAGGCTGTCGTGCCGGCCGTCCTT11.9230769230769231No Hit
GGTTGAGCGTGACCGATTTCTTGTTCCGGCTCTGCGAATACCACCACAGG11.9230769230769231No Hit
GGTTTGGAAGCCGGGTGCACGCCCGGCAGATCGGGCAGGGCATCCAGACG11.9230769230769231No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT11.9230769230769231No Hit
TCTACACTCTTTCCCTACACGACGCTCGTTTTTCAGGACCTGTTGGTACC11.9230769230769231Illumina Single End PCR Primer 1 (96% over 29bp)
GATCTCGGTGGTCGCCGTATCATTCCCATGTACTCTGCGTTGATACTGTC11.9230769230769231Illumina Single End PCR Primer 1 (100% over 24bp)
GTCCTGGACCTTGTACTCCGCAGTCATGCTGGTTCCTTTGATGATGCGAG11.9230769230769231No Hit
CGCCTGCGCGAGCGAACCGTTGAGGTCGATCGCGCGGCACGCATCCTCGA11.9230769230769231No Hit
GGTTTGGAAGCCGGGTGCACGCCCGGCAGATCGGGCAGGGCATCAAGACG11.9230769230769231No Hit
GTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGT11.9230769230769231No Hit
CTGCCACAGCGGCATCATCGCCTGCAAAGACGGATGCAGGCCGTAGCGGT11.9230769230769231No Hit
TTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCAAATTGAGCTGGCT11.9230769230769231No Hit
GCATCGGCGCGCGCCTGGCGCATGGGCTTGCCGGTGTCGCGGGCTTCCAG11.9230769230769231No Hit
CGGCGGCCCGTGAGGATCTTGTGCGGATAGCTCTGGTGGCCGACGTCCCA11.9230769230769231No Hit
CCGCGCAGTCCAAGCGCATGAACGTGGTCGCCTCCAACCTGGCCAACGCC11.9230769230769231No Hit
ATTCATTGGTTTGTGCTAGCGGCGTAATGTTTTGTTTTTAAAACAATCTG11.9230769230769231No Hit
GTTTTGGAAGCCGGGTGCACGCCCGGCAGATCGGGCAGGGCATCCAGACG11.9230769230769231No Hit
TGTAGCGCGGGTCCACCACGATGACCTAGCAGCCCGTTTCCTTGGCATGC11.9230769230769231No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATATCTTCCCA11.9230769230769231No Hit
TTCTTCCACCAAGAGCCCTGAAGCCGCCCGTTCGGCTTCGCTCATGGCGG11.9230769230769231No Hit
CCGGTACCGTCATCGACCCCAGGTATTAACCAGAGCCATTTCTTTCCGGA11.9230769230769231No Hit
GGGCAAGAGGTCCCGGGGAAACGAAACTGGAAACCGACCGCCGCCATATC11.9230769230769231No Hit
TACACCGCGTGCCCGAACGACAGCATGCCGGACTGCCCCAGCAGCATGTT11.9230769230769231No Hit
TCTTGGTTGCGCTTGCGCTGGCAGGGGTCAGCTTGCTCTCGACTTCGGTA11.9230769230769231No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC11.9230769230769231No Hit
GCATAAGCGCTACCGGCGCGCGAAAAGAAACCTTGGGCGGTGCAAGGCCG11.9230769230769231No Hit
AGCTTCTGCAGTGCCACGCGACGCATCCACAACGCGATAGCGAGGAAAAC11.9230769230769231No Hit
GTATAAAGAGTTGTGCCTGCGTCTCCCACGAGAGAACGGCCCGGTTCGAT11.9230769230769231No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA11.9230769230769231No Hit
GCACAACCAGGTGCACATCTGGCGCCGGAGCGAGTAACCGGCCATCCCTT11.9230769230769231No Hit
GATAACGACCGCGTGGCCATCACCGGCAAGTTCAAGGTCGACCGGGGCGT11.9230769230769231No Hit
GCTATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCT11.9230769230769231No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)