FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003627192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003627192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52
Sequences flagged as poor quality0
Sequence length100
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAAGGTGCGGCAAGTTAGGAGGGCTGTTCGGCGCTTTGCAGCCAAGGC35.769230769230769No Hit
TCAGCCAGATGACGCAGGACTTCCCGGAACTGCCCGAACCCTATATCAAC35.769230769230769No Hit
GCACAGCCTCGTGTTCCTTGCGGGCCGGGGCGTTGCTGGCGCTGCCGTTT23.8461538461538463No Hit
GTGGCATGGGCGCGGGCGACGTGAAACTGATGGGCGCGATCGGCGCGTTC23.8461538461538463No Hit
GTCCAAGCACAGCAACGAGCAGTCGTACCTGATGCGCAAGTGGGTGTCGT23.8461538461538463No Hit
GTACATGGGCCGTCTACCTTGCCTCAGATTCTGTCGGTACCAGAGATCGG23.8461538461538463No Hit
TGACCGAAGAGCAGGAAGCGAGGATGATCCTCTTGCTTGTCCCTCCAGCG23.8461538461538463No Hit
GTGGAAGCAGCCGGACATGATCCAGTACGCCATTCAGGCCGCCAAGTACC23.8461538461538463No Hit
CTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATA23.8461538461538463No Hit
TGTACGGATCGTTCGGCGCGATCTTGGCGACCAGCTCGCGCAAGGTCGGG23.8461538461538463No Hit
GTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCTTCTTTC11.9230769230769231No Hit
GCTGGTGCTGATGCTGATCTTCCGCCCGCGCGGGCTGATGGGTACGCGGG11.9230769230769231No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGATA11.9230769230769231Clontech Universal Primer Mix Long (96% over 26bp)
TGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGAC11.9230769230769231No Hit
GTGCATGCGCTTCATGGGGGTGACGGTGTCTTCCACCACGCGGTCGACCG11.9230769230769231No Hit
ATATGGGGCAGGCAGGATCGACAGGGGTGAAGGCCACTGTGCCGGCAAAG11.9230769230769231No Hit
TATCTGACCATCGAGCATAAGGGGAGCATTCCGGTTGAGCTGCGCCCGGC11.9230769230769231No Hit
GCATTTTCTTTCACAGCTTCCCGTTACGGTTTTGACATTCCGGAAATTTG11.9230769230769231No Hit
TATCAACGCAGAGTACATGGGAATGATACGGCGACCACCGAGATCCTGTC11.9230769230769231Illumina Single End PCR Primer 1 (95% over 24bp)
GTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCC11.9230769230769231No Hit
ATTTACCAGGGCGAAATGAGCGCGTTGCAGAACGCAAACCAGCAGCAAAC11.9230769230769231No Hit
GTGCATCTGGCCGCCAAGCGACAGGTCTGCACCGGCACCCTTGAAGCGGA11.9230769230769231No Hit
GGTACGCAGCCATTCGGCATGATCGACCTGCCGTATGGGGAGCAAGTGCT11.9230769230769231No Hit
GCGTGGCCACTGAACAACATTGCATCGACGTTAGAAGTCTTTGCCAAAAC11.9230769230769231No Hit
GCCAGCAGGCCTTCGGTTTGTATGGGGCCCTGATCATCGATCCCAAGCAG11.9230769230769231No Hit
GTCCTGAGTAAACGGCAAATTTCCACAAGTTCTGGCTCTGTCCTTTTTCA11.9230769230769231No Hit
CTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTC11.9230769230769231No Hit
CCGCAACTGGCCCCACGCATTTCGATCCAGCGCGCCGTGTTGCCCATCGA11.9230769230769231No Hit
GGTGGGGCGGGCTGGTGTGGATTGCGGCCGTGGTGCTGGCCTTCGTGCTG11.9230769230769231No Hit
CGCCATGGGCGAGCGCGTTGCCAACGACGAAGCCAAGATCGACGAGTACA11.9230769230769231No Hit
CCCGTGCGAAAATCGGGCGTTGCCGATCTTTTGCAGGAACCCCCCATGCA11.9230769230769231No Hit
CTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGC11.9230769230769231No Hit
GGGTAGCGTGTTCTGACATGGATACAGAGTGGTCGATTTTGCTCGGATCG11.9230769230769231No Hit
GCTGAGCTCTGTCTGGCTTCCGCTAGTCGCGGCTCTCGCGCGGAAAGGTT11.9230769230769231No Hit
GTCGTCCATTCCCCCTTCGTGTTGAAGCCTTCTCCTTTGGAGATGGCTCG11.9230769230769231No Hit
TCTTTGAGATGCTCGAGAAAAGCGGAAACGCGGGCGCTCAGCAACCGCCT11.9230769230769231No Hit
TAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTGCAG11.9230769230769231No Hit
CTGTAGTGGGGGATAACTAGTCGAAAGATTAGCTAATACCGCATACGACC11.9230769230769231No Hit
GTCCGGCAGCGCGTCGCATACCGCCTGGTGCGAAAGCGCATCGGCGCCCT11.9230769230769231No Hit
GCGAAGTCGTTACCACGCCGGCCAGCAGCGGATGCATGTGTTGTAGAAAG11.9230769230769231No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)