FastQCFastQC Report
Wed 11 May 2022
EGAF00003627225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003627225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7888182
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGG347230.4401901477425344No Hit
GTACATGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTT321860.40802811091326235No Hit
GAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGG319460.4049855847646517No Hit
TCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCCAGAGTTTTCAGA288000.3651031378332802No Hit
CCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAG280760.3559248506183047No Hit
GGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCC267160.3386838691095109No Hit
GTACATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGC246150.3120490881168817No Hit
CTACATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCAGGCAG238190.301958043057323No Hit
ATAATTGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCATCTTCC217640.2759064129098441No Hit
ATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCA178900.22679496999435358No Hit
GGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGA174090.22069724050484638No Hit
GGATAGGAGCATCATCTTCCATCCCCATCCACCTCCGCTCTTTATCTTGA145880.18493488106638514No Hit
GGACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAGCATC141420.17928085330688365No Hit
GCCTTTGTAGTTACTCTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTT137430.17422265358481842No Hit
TAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCT136480.17301832031766No Hit
GTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTTGGGCT132730.16826437321045584No Hit
GTACATGGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGATT126350.16017632453206582No Hit
GTGTGATAGCGCCTCCAGCAGTTTCTTCACAGCTTCAGAAGCCTCTGGTC124920.1583634860351853No Hit
GTCATGAAGCTGGTAACTATCTTCCTGCTGGTGACCATCAGCCTTTGTAG123960.15714647557574102No Hit
CTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTC116730.1479808655530514No Hit
ATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCAGGCAGGGAG114800.14553416744187697No Hit
GAACCAGAGACGGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTG113840.14431715698243272No Hit
GTATCAACGCAGAGTACATGGGACACTTTGTATGGCAAGTGGAACCACTG113810.14427912540557508No Hit
GAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAG113730.14417770786728804No Hit
GCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTC113100.14337904475327776No Hit
GGCTAAGGAAGTGTGTAAATGAGCTGGGACCAGAGGCTTCTGAAGCTGTG110820.14048864491209762No Hit
GTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGAGGGAG110180.13967730460580144No Hit
GGTCACTACATTTTAGGGCATTTGATCTATAATTGGTAGAACAGGTTTCA106330.13479658557573848No Hit
CACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTT105860.13420075753830224No Hit
GGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAGAGCA103300.13095539631311753No Hit
GTACATGGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGG103030.1306131121213988No Hit
GCTTTATTTGTCCTTTTCACGGGTCACTACATTTTAGGGCATTTGATCTA102920.13047366300625415No Hit
GCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTT101930.12921862096995226No Hit
GGTTTCAGGCAGGGAGGATAGGAGCATCATCTTCCATCCCCATCCACCTC100190.12701278951220954No Hit
CCTTTACCTCTGGACAACATTCTTCCCTTTATGGATCCATTAAAGCTTCT99860.12659444216677557No Hit
CATTTACACACTTCCTTAGCCCCTCCACAAGGTGCTCAACAGAAATGCCC99520.12616341762905572No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98160.12443931947817632No Hit
AGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAG91800.11637662518435805No Hit
GTCTTGATCAGGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGAGGGTG91590.11611040414635464No Hit
TTTCACGGGTCACTACATTTTAGGGCATTTGATCTATAATTGGTAGAACA90610.11486803930233862No Hit
CCATGAAACTCGCTGTCACCCTCACCCTGGTCACACTGGCTCTCTGCTGC90520.11475394457176571No Hit
CATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACGGGCCAG89520.11348622534317794No Hit
AAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCTTCTAA89310.1132200043051745No Hit
GAGGATAGGAGCATCATCTTCCATCCCCATCCACCTCCGCTCTTTATCTT88940.11275094819059703No Hit
GGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGA88720.11247204996030771No Hit
ACTCAAAGCATGGCAGCGGCAGAGGCTGGAGCAGTTGGGGATCTTCAGCT88600.11231992365287717No Hit
GCTACTGCCTTCCTCATCAACAAAGTGCCCCTTCCTGTTGACAAGTTGGC86950.11022818692570735No Hit
GTATCAACGCAGAGTACATGGGATACTAGCCCACCAGACTCAGAGACGGA86720.10993661150313215No Hit
GATCTATAATTGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCAT86490.10964503608055695No Hit
CTATCTTCCTGCTGGTGACCATCAGCCTTTGTAGTTACTCTGCTACTGCC86190.10926472031198063No Hit
ATCTATAATTGGTAGAACAGGTTTCAGGCAGGGAGGATAGGAGCATCATC83780.10620951697108408No Hit
TCTAAATGCTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTT83270.10556298016450431No Hit
TAGTTACTCTGCTACTGCCTTCCTCATCAACAAAGTGCCCCTTCCTGTTG82900.10509392404992683No Hit
TGCTTGTGGTTTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGC82450.10452345039706235No Hit
GAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCCTGGTC82290.1043206153204883No Hit
AAACCCTCCTCATGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTT81640.10349659782190625No Hit
TTGTATGGCAAGTGGAACCACTGGCTTGGTGGATTTTGCTAGATTTTTCT80680.10227958736246197No Hit
GAGTACATGGGACACTTTGTATGGCAAGTGGAACCACTGGCTTGGTGGAT79460.10073296990358489No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)