FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003627233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003627233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA34.838709677419355Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT34.838709677419355Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCTGTCTCTTAT34.838709677419355No Hit
ACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGA23.225806451612903No Hit
GCCACTGATTCCCCAAGACCCCCCATGGCAAGATCTTAGAGGAGAATGAG23.225806451612903No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG23.225806451612903Clontech Universal Primer Mix Long (96% over 26bp)
GAATGGGAAATGATCTTAGTCTGAATCGCAAGATTGCATGTTTTGCTTTT23.225806451612903No Hit
ACGTTATATCTATAGAACGCGAATTGGAGTCGCATCGCTGCAGGGTTTGA11.6129032258064515No Hit
GGTGTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAA11.6129032258064515No Hit
GCAGTGGCAGAGAAAGCCTGAAAACCTCAGTGGCTTAACACATGTTTATT11.6129032258064515No Hit
GTGGACACCACCCCAGTCTTCTCTTCCTGGCCATTCCGGAACCACCTGAC11.6129032258064515No Hit
GCGTTGATACCACTGCTTTCCCATGTACTCTGCGTTGATACCACTGCTTT11.6129032258064515Clontech SMART CDS Primer II A (96% over 26bp)
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT11.6129032258064515No Hit
ACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATTTAT11.6129032258064515No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG11.6129032258064515No Hit
TCGGGGATATCCCAGTACTGACGAAAAACCTTCGGCGCCATAGCCCGCAA11.6129032258064515No Hit
GAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACC11.6129032258064515No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTGATACCACTGCTTC11.6129032258064515Clontech Universal Primer Mix Long (95% over 21bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTGTTTT11.6129032258064515No Hit
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGC11.6129032258064515No Hit
GGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGA11.6129032258064515No Hit
GGCTGCCGCAGTGCTTGTCACAGACTCGACCACACTGGCCGAAGCTGTGC11.6129032258064515No Hit
GCTTTAATTGTTCAATCAAGATTTGTAGGTAAGCATCAGCTTCTGTAAGT11.6129032258064515No Hit
ACATATACTTCATACAGTGTATTTCTCCCTTTAATTATATTCCGGTAGGA11.6129032258064515No Hit
GAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTC11.6129032258064515No Hit
GATTTACACCCTCCCCCGGATTTTCAAGGGCCAGCGAGAGGGTGTAAATC11.6129032258064515No Hit
ATGATAGGAAGAGCCGACATCGAAGGATCAAATAGCGACGTCGCTATGAA11.6129032258064515No Hit
GTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATTCCTAG11.6129032258064515No Hit
CGCCGGAAACTTGGCGCGCAGGGCGTCGATCACCCGGGCGGTGTCGTCCA11.6129032258064515No Hit
AAGGTGAGCTGCCCGGCCGCCTGGGTCGAGATGCCCATGAGCCGCGCGCC11.6129032258064515No Hit
GGGCTGATTTCGCGCGGGCTCACGCCCGCCCCGTCGTGAGGTGAGATTTC11.6129032258064515No Hit
GCTTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG11.6129032258064515Clontech Universal Primer Mix Long (96% over 26bp)
CTCTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGT11.6129032258064515No Hit
ACTCATGACTGATCGCGAACACATCCAAACCTTGCTGACGGTCGCGCGGC11.6129032258064515No Hit
ATGGACGTCTGCTTCTCTGCCAAGGGAGAGAGTGAGGTAGGCCTGGGCCC11.6129032258064515No Hit
AGTTAAATGTTTTGAGTATTATTCCAGAGAGCAAGTGGCAGAGGCTGGAT11.6129032258064515No Hit
ATTTGAGTCTGTAAGTCTCTTCATAAGAATGTCTTTGAGAGAATGTCTGA11.6129032258064515No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG11.6129032258064515Clontech Universal Primer Mix Long (96% over 26bp)
GGAGATGAGCCCGACGTCCATCGCGTCGAGCGCCCAGCCCGTGCCCTGTC11.6129032258064515No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA11.6129032258064515Clontech Universal Primer Mix Long (96% over 26bp)
TCTCCAGGGGCACCATTGAAATCCTGAGTGATGTGCAGCTGATCAAGACT11.6129032258064515No Hit
GGGAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA11.6129032258064515Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCCTGTCT11.6129032258064515Clontech Universal Primer Mix Long (96% over 26bp)
GTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAAC11.6129032258064515No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA11.6129032258064515Clontech Universal Primer Mix Long (95% over 21bp)
GGCCATCCGTATTTCCTGCCGCGCCACCTCCGACAGCAAACTCGCTTGTT11.6129032258064515No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG11.6129032258064515No Hit
AGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTA11.6129032258064515No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGACTCTTAT11.6129032258064515Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGCGGGGACAGGCGGGGGACCGGCTATCCGAGGCCAACCGAGGCTCCGCG11.6129032258064515No Hit
CTGTTTGGTTTTTCATTTGCTCTGTAAAATTATTATGGGCCAAAGACTTA11.6129032258064515No Hit
GACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAA11.6129032258064515No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)