FastQCFastQC Report
Sat 23 Apr 2022
EGAF00003627234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003627234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTAT34.838709677419355No Hit
TATGATCCTTTTTGATTTATGGTTTTCAATACTCATTCTCCTCTAAGATC23.225806451612903No Hit
GTGTGATCCTATATTAGAGAAGTGGAAAACCAGGCTGAGAGATGTATTTC23.225806451612903No Hit
GGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTC23.225806451612903No Hit
TTTTGATGAGCCTCCATGGTATGGTGGCGACAGGGCAAGACCGTTTGATG11.6129032258064515No Hit
GCATATGCCTGTAATCCCAACTACTCAGAAGGCCGAGGCAGGAGAATTAC11.6129032258064515No Hit
AGCGTATGGCGCTCGGGCCGCTCAACGTGAGCGGGCAGGCGCTCATCATC11.6129032258064515No Hit
CACCAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTT11.6129032258064515No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT11.6129032258064515No Hit
ATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAA11.6129032258064515No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA11.6129032258064515No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG11.6129032258064515No Hit
ACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGG11.6129032258064515No Hit
CTGCAGGCTGGAGGTCCCGGCAGGCACAGGGCTCACACCTTGGGTTTTGC11.6129032258064515No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC11.6129032258064515No Hit
GAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTTTCAGTACGAATACAG11.6129032258064515No Hit
TCCTCGGAGTGGAGAGGTTTACACCTGCCAAGTGGAGCACCCAAGCGTGA11.6129032258064515No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG11.6129032258064515No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGGTGAT11.6129032258064515No Hit
CGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATC11.6129032258064515No Hit
CAACGCAGAGTACATGGGAAAGCAGTGGTATCAACGCAGAGTACATGGGA11.6129032258064515No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT11.6129032258064515No Hit
GGATGCCATTGGGTACGGAACCGCTAAGGCATCAGCGGCTCTAGGCCAAG11.6129032258064515No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT11.6129032258064515No Hit
GCGCAATATTCGCCTGTCGTTCTGTTACCCGACTCCTGAGCGCATCAAAG11.6129032258064515No Hit
TAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAG11.6129032258064515No Hit
ATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGG11.6129032258064515No Hit
TAGGGAACGTGTGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACC11.6129032258064515No Hit
GTGTTGGGTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCG11.6129032258064515No Hit
CCTCCAGGAAGCGAGAATGCAGAGTTACCTCAGTGATATCAAGAAATTCA11.6129032258064515No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11.6129032258064515No Hit
CCTCTTCGTAAGATGGGACAACCAGTTTTACCTCGGAGATCCTCAGAAGA11.6129032258064515No Hit
GCTGGGAGCATAAGCTTCAGGTCCAGGAAGATGGGTTTGGATCCAGCCTC11.6129032258064515No Hit
CACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAATCCTGTC11.6129032258064515No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11.6129032258064515No Hit
GTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATAC11.6129032258064515No Hit
GGCACGGGCTGGGCGCTCGACGCGATGGACGTCGGGCTCATCTCCCTGTC11.6129032258064515No Hit
GCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTC11.6129032258064515No Hit
GAGAAAGAAGGTTAAGCTGGCTGTCCTGAAATATTATAAGGTGGATGAGA11.6129032258064515No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG11.6129032258064515No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTAAAAACATGA11.6129032258064515No Hit
GTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGTTG11.6129032258064515No Hit
ATGCTGCACTGTGAACGTGATGATCCCGCGACGCCTCGCCTCGTCAAGAA11.6129032258064515No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC11.6129032258064515No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC11.6129032258064515No Hit
GAGCGCAACCTCAACCGCATCACCGCAGCGCCGACGGTGATGCGGTTGAG11.6129032258064515No Hit
GCAGAAGGGCAAAAGCTCGCTTGATCTTGATTTTCAGTACGAATACAGAC11.6129032258064515No Hit
GAGGTGCTGCGCTACGCCAGGCGCGGCCAGGAATGTATTCTCATCGGTCA11.6129032258064515No Hit
TCTTTGTCACATTGGGGCACAATGCTTCCCACAGGCCTGGCCTAGGCATA11.6129032258064515No Hit
AATGGCGCCAGCGTTCCGGACCCGAGCAGCGACTGCGGCAGCATCAGCCG11.6129032258064515No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA11.6129032258064515No Hit
GCGCGCCCAGCCCCGCTTCGCGCCCCAGCCCGACCGACCCAGCCCTTAGA11.6129032258064515No Hit
CACCCTGCCTCCTTCCCGCCGCCCCCTGGGGGTTTGGATTCAGGATTTGT11.6129032258064515No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCT11.6129032258064515No Hit
AAATATGATACTCTGGGGAGGAAATGCTTGGTTTGTGTACAAGGAGACCA11.6129032258064515No Hit
GGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATT11.6129032258064515No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC11.6129032258064515No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGAA150.031.3333345
GAAGCAG150.031.3333349
GGAAGCA150.031.3333348
GGGAAGC150.031.3333347
TGGGAAG150.031.3333346
ACATGGG200.023.53
GTACATG200.023.51
TACATGG200.023.52
CATGGGA200.023.54
Produced by FastQC (version 0.11.5)