FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003627278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003627278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTGTATACCTATAGAAAGCGCGCTCTCCTTCGCATCCAGGGCTTTTA125.0No Hit
ATTCCTTAACATTCTCAGGTTTTAAAAAATCATTTAAATGGAAGGGTCAC125.0No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT125.0Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGATACGGCACCACCGAGTCTAATCTTTCCTACACGACGCTCT125.0No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)