FastQCFastQC Report
Tue 10 May 2022
EGAF00003627289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003627289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1777014
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTATTACTTTGCAAGGGGCCCTTCAGAAGTCGCTGGGCTCAGAAGGCT31500.17726365689859505No Hit
GAACAGATCCGGGGACTCTCTTCCAGCCTCCGACCGCCCTCCGATTTCCT27640.15554182465641803No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA27310.15368477682224227No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG25950.1460314887783664No Hit
CTCCTACACCTACCTCTCTCTGGGCTTCTATTTCGACCGCGATGATGTGG25900.1457501178944004No Hit
GGCTTAGGCAGAAGCCCTATTACTTTGCAAGGGGCCCTTCAGAAGTCGCT25220.14192347387246246No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA24700.13899721667921583No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA23400.13168157369609917No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT22780.1281925747349205No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC22530.12678572031509036No Hit
GTACATGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAG22530.12678572031509036No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT21220.11941380315518055No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT20520.11547461077965622No Hit
CTCCGGGACCATCTTCTCGGCCATCTCCTGCTTCTGGGACCTGCCAGCAC20410.11485559483493096No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG19520.10984719310033572No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT18960.10669583919991628No Hit
TGTTAGGATAGTTAAGAAAGCTGCCTATTGGCTGGAGGGAGAGGCTTAGG18450.10382585618346281No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA18150.10213763087966667No Hit
GTCCAAGGCTTGTTAGGATAGTTAAGAAAGCTGCCTATTGGCTGGAGGGA17930.10089959899021617No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA17890.10067450228304337No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)