FastQCFastQC Report
Tue 10 May 2022
EGAF00003627321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003627321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189480
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG9690.5113996200126663No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9140.48237280979522906No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGCTTCCATCTCGTATG7050.3720709309689677No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGCTTCCATCGCGTATG6590.34779396242347477No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5810.30662866793329113No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT4630.24435296601224404No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA4140.21849271690943636No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGCTTCCATCTCGTATGCCG3640.19210470762085707No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGCTTCCATCGCGTATGCCG3530.18629934557736963No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3430.1810217437196538No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA3150.1662444585180494No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT3090.16307789740341988No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC2930.1546337344310745No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG2820.1488283723875871No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2770.14618957145872916No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG2570.13563436774329743No Hit
GTACATGGGAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCC2480.13088452607135317No Hit
GGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCT2480.13088452607135317No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA2470.1303567658855816No Hit
ACACATCTCCGAGCCCACGAGACGAGCACGCTTCCATCGCGTATGCCGTC2430.12824572514249524No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT2390.1261346843994089Clontech SMART CDS Primer II A (100% over 26bp)
GATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGG2280.12032932235592148No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA2090.11030187882626134No Hit
ACACATCTCCGAGCCCACGAGACGAGCACGCTTCCATCTCGTATGCCGTC2060.10871859826894659No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2050.10819083808317499No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG2050.10819083808317499No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT2020.10660755752586026No Hit
GCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACT1990.1050242769685455No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT1960.10344099641123074Clontech SMART CDS Primer II A (100% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)