FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003627513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003627513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA67.0588235294117645Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATGTAAATTGGAAGTTAACTTATGCACGCTTAACTATCTTAACAAGCT22.3529411764705883No Hit
GGGCCAAGCTGTGCAAGGACTGCAAGGTGGCTGACGGAAAGTCCGTGACA22.3529411764705883No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT22.3529411764705883Clontech Universal Primer Mix Long (100% over 23bp)
TGTTCACCCACTAATAGGGAACGTGAGCTGGGTTAGACCGTCGTGAGACA22.3529411764705883No Hit
CACCTAGATGATGTGACTTTTTTCTTCTGGAGTTTGTCCACAGTGGGAAT22.3529411764705883No Hit
CGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATG22.3529411764705883No Hit
GTTTTAGGCTTAGGAGATATTCTGTCGAACAAGACAGGAACAGTCCTGCC22.3529411764705883No Hit
GGACTCGAACTCACTACCTCAGGTGATCCGCCTGCCTCAGCCTCCCAAAG22.3529411764705883No Hit
CTGCACGAGCCCTTCCGGGTTGCGCGTGTGCCGCACCAGATGGCCCGCAT22.3529411764705883No Hit
CGTGTTCACAACCTACCTTGGGGTGTTCATCATGATGACTTTTCCTCCGT22.3529411764705883No Hit
GGCCTGACGGCGGACGGCTGGCTCGTCAGCGGCTGCAACGTCGAAAACGC22.3529411764705883No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC22.3529411764705883Clontech Universal Primer Mix Long (96% over 26bp)
AAGCTCTCATGCCCACAGCCAAGCCAGCCAGGTGGGGACAGCCCAGGGCG22.3529411764705883No Hit
CATTTACAACTGCAGTTGACAAAATACAGGCTGCCAGGGAAATTTGAATT11.1764705882352942No Hit
GCGCCCATCTCTTCGGCAAACACCTTGTAGCGCAGGCGCTGCGCTTCGGT11.1764705882352942No Hit
ATTATAGATTTTTACATGGTCTTTATTTCATTACTTTTGTCTTATATACC11.1764705882352942No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT11.1764705882352942Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGGAGAAAAGCCACCACATCCATCTCATGAAAGATGAGTGCCGAAC11.1764705882352942No Hit
ATGCCGGAAATACCGCGTCCAGGGTGACATTCTGCTTGACGGCAGATCCG11.1764705882352942No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG11.1764705882352942Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AACTTGCATGGTAATTACTGCATTAGCTTTCTTGAATAGTTTTCTAACTT11.1764705882352942No Hit
GGACAGTGGATTCTACTGTAAATGTGGGAGACCATTAGAAGATTGGAAGT11.1764705882352942No Hit
AAGCTGCTGTGGAAGCTCTGGTCACCCACCTGGAAGTTCGACGACGCCAC11.1764705882352942No Hit
ATGCCGGACATACCGCGTCCAGGGTGACATTCTGCTTGACGGCAGATCCG11.1764705882352942No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTGTTTACACA11.1764705882352942No Hit
CTGCGGCGCCGGGCCGAGTCCGTCGCGACGCGGCTGTCGGCCCGCACGAT11.1764705882352942No Hit
AATCAGGACTGCCCAACAAAATATTCTGTCAGTCATTCATGATCTGAATT11.1764705882352942No Hit
TTTTTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG11.1764705882352942Clontech SMART CDS Primer II A (95% over 23bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATGAAAAAAAAAAAAAAA11.1764705882352942No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA11.1764705882352942No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTGTCTCTTA11.1764705882352942Clontech Universal Primer Mix Long (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCTGTGGTATCAA11.1764705882352942Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCAGTGGACTGGCGTGCAAGATGCCCAGAAACACATATCAGCCATCATAG11.1764705882352942No Hit
CTATTACTCCTTTGTGAAATCCACTAAAAATCACTGATTCTCCTTTGGTA11.1764705882352942No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG11.1764705882352942No Hit
GGCTCAGGGTGATGCAGAAAATGTGATGTTGCCAGGCCATCCAAATAAAG11.1764705882352942No Hit
GTGATGACATTCATGTACAAGAACTGTCTGATGCCCCCATTACAATAACC11.1764705882352942No Hit
CTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT11.1764705882352942No Hit
ATTAAGAAGCTTTTGCAGTAGTCCACATGAGCTATTATAAGGGCCAGGAT11.1764705882352942No Hit
CCAGAAGACAGAGCACATTTCTCACTACTTCTGCTGAAGGAAAACTCACC11.1764705882352942No Hit
GCTCACAACACCGGACGCCGCGCGGCAGGGCTGTGGACCTGGGGTGGTTT11.1764705882352942No Hit
GAAGAAAGAGAAAGGAAGGGAGGAAGGAAGGAAGGGAAAAAAGCAAAACA11.1764705882352942No Hit
TTTCCTTTCCCCAGAAGAGATCCCTAACCTATTGTTTTATTGACAGCCTT11.1764705882352942No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA11.1764705882352942Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG11.1764705882352942Clontech Universal Primer Mix Long (96% over 25bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC11.1764705882352942Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACCTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAAAAAAAGAAAAAA11.1764705882352942No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11.1764705882352942Clontech Universal Primer Mix Long (96% over 26bp)
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT11.1764705882352942No Hit
CTATAGCCTGGCCCATTCCTACCTCGGAGGTGGAGGCCGGAAAGGTCGCA11.1764705882352942No Hit
TCCTCATGCTACTCTTCTAAGTCTTCATATCTGTCTCTTATACACATCTC11.1764705882352942No Hit
TGTTAAACCAGCCTATTGTTGTTTTTAAAAATATATTCCCAGTCTGCCTT11.1764705882352942No Hit
GAATTATAACACAGCATGAGAAAACTCAGGACTTCAAACCTAAAAACAAC11.1764705882352942No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGCTG11.1764705882352942Clontech Universal Primer Mix Long (96% over 26bp)
CAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGTTGGGTCAAAT11.1764705882352942No Hit
AGATTACTGAGCCCCTACCATAAGCTGGGAGCTAGGTACTAAAGATACAA11.1764705882352942No Hit
GGTCAGGACGGTCAGGATGAGAAGAAGAAACCGAAAGACTAGGAGATATG11.1764705882352942No Hit
GAAATCCGGGGTAAAGATGTTCTGGTAGTCGAGGATATCATCGACTCAGG11.1764705882352942No Hit
GGCTGGAGTGCAGTGGCACGATTTCTGCTCGCTGCAAGCTCCGCCTCCTG11.1764705882352942No Hit
AAAGATGACCAGGAGGCTGTGCTATGTTTCTACAAAACCGCCAAGGACTG11.1764705882352942No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC11.1764705882352942Clontech Universal Primer Mix Long (96% over 25bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTATAACACTCG11.1764705882352942No Hit
TCTTTTACAACATCAAAAATTGGTTCCCTTATTGATGTCAATCAATCCAA11.1764705882352942No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTCTCTTATA11.1764705882352942Clontech SMART CDS Primer II A (96% over 26bp)
CTACCAGCATAAAGTGACACATTCAAGTGGAAAAAGTAAGTTGTTAAATG11.1764705882352942No Hit
ATGTTGCCTTCCACTTTAACCCACGCTTCAATGAGAACAACAGGAGAGTC11.1764705882352942No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)