FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003627514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003627514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC33.5294117647058822Clontech SMART CDS Primer II A (96% over 26bp)
TCGTAGGTGCCTGCATTCTTGTAGGCGCTCACGTAGCCACTGTCGTCCAG22.3529411764705883No Hit
TTTGTGGGTAGGGCTTAGAAAGGAGAGACACAGCCTAGGAGCTGGGCTCA22.3529411764705883No Hit
TTCCCGTGTTGAGTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCC22.3529411764705883No Hit
GTGGGCAGCCGGGTAACGGAGGAAAAGTCATCATGATGAACACCCCAAGG22.3529411764705883No Hit
TTGCCGTTGACGGCGACGACCGCGACGAGCGCACCGCCGCCGGAGCGGAT22.3529411764705883No Hit
TCCCCACATCTGGCTAATTTTTGTATTTTTTGGTAGAGACAAGATTTTGC22.3529411764705883No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT22.3529411764705883Clontech Universal Primer Mix Long (96% over 26bp)
TCCTGCTGTTCTACCGCGCGTGGGCCGACGCGCAGCCGACGGTGAGCCTC22.3529411764705883No Hit
CTTATACACTTACACTTTATGCACAAAATGTAGGGTTATAATAATGTTAA22.3529411764705883No Hit
AATGCATACATGGCCAGCTGAGGTAGAACAGCTAATTCTATGCTTTCTTA22.3529411764705883No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT22.3529411764705883Clontech Universal Primer Mix Long (96% over 26bp)
ACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTG22.3529411764705883No Hit
GGTATCAACGCAGAGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC22.3529411764705883Clontech SMART CDS Primer II A (96% over 26bp)
GGGACCCTGTTCATGTTCGTTTCCCTGGCCGATCTCATCGGGCGCTTCCT22.3529411764705883No Hit
GATTTGTAATAAGGAGAATATAAACAGGGAAACCTTTGAGGAAGATGCTA11.1764705882352942No Hit
GTGTATTTCGGGGTTGGGGGCGGGAAGAAAGTAAAATAGAAATTAAGGAC11.1764705882352942No Hit
GAATATTTATGTTAACTCCCTTATTTTACAGCTAATGAAGTTGACCCTAG11.1764705882352942No Hit
TCCATAACCTGCAGGGCGAGAAATTCTACAATGCAGGCCTGGCCTACTGT11.1764705882352942No Hit
CATATAAAGATATAATAAAAGGATACGGTATTTATTGCAACAGCAAATTA11.1764705882352942No Hit
GGATTCGAGTAAATAAAACTGGCAGGACTTTTCTTAGGCTCTTAGAGTTT11.1764705882352942No Hit
CATAGTGTTGAATGTTGAAAGAACAAATTAGGTTGAAATCTAAAGTGCAA11.1764705882352942No Hit
CATGTATGAAGGTGTAGGAAGAATGTTTATTCTTCAGTCCAAGGAAGCAA11.1764705882352942No Hit
AACCCCATCTCTACTAAAAATACAAAAAATTAGCCGGGCGCGGTGGCGGG11.1764705882352942No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11.1764705882352942Clontech Universal Primer Mix Long (96% over 26bp)
GTCTCCAACATTGTTCTTTTTGTTTAAAATTGCCTTGGCTATTGGGCTTT11.1764705882352942No Hit
GGGTTTACAGACTAGATCTGGACAGTCTCACCAGGGTGAAAGCTCGGAGA11.1764705882352942No Hit
GTCTCTTTGGGCGGAAGACAGGTCAGGCCCCTGGATACTCTTACACAGCC11.1764705882352942No Hit
AAATTTATCTTCTACTTCAAAGCCTAAAAATTTGACTTCAAGTTCAATTT11.1764705882352942No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG11.1764705882352942Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.1764705882352942No Hit
GCCTACAACTCAGGACATTTCCTATTTTAAAAGCCCGGGCTTCCGATTCC11.1764705882352942No Hit
CATGGGAAGCAGTGGTATCAACGTAAAAAAAAAAAAAAAAAAAAAAAAAA11.1764705882352942No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC11.1764705882352942Clontech Universal Primer Mix Long (96% over 26bp)
CCCCGGCGACGGTTCGGTCGGGGTCGGGTCCGAGGGCCTCCTCGCCCGTG11.1764705882352942No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA11.1764705882352942Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTATA11.1764705882352942Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG11.1764705882352942Clontech Universal Primer Mix Long (96% over 26bp)
GATGATTTTAAACAGGATTTTCACGGATTCTGGACTCATGAGATTTGAAA11.1764705882352942No Hit
ATATCATGGTATATGAAGCACTGGTGAGGTCTATGTCACCAGAAATTCCC11.1764705882352942No Hit
GAAATAAACTTTTAGTGTAAGTATGTCCTGTGCAATATTGAAGATACACT11.1764705882352942No Hit
GTCGCGGATCTGTCACGTAGCGCCTCTAGAATCGCTTCGAAATCCACCCT11.1764705882352942No Hit
TACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTCTGTCTCTTA11.1764705882352942Clontech Universal Primer Mix Long (96% over 26bp)
GTGCGGGGCGCTTCCTGCGGCAGGTTGTGGCCGGCGCCCGGCACGAGCCG11.1764705882352942No Hit
TAGTATATGTGTGTCGAGTGAGCTAAAGGTGTACAATGAGGACCCAAGTG11.1764705882352942No Hit
GATCTAGTCTCTGAAAATTCACCGACATTTTTGGCTTGGCGCTGTGGCTC11.1764705882352942No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTG11.1764705882352942Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11.1764705882352942Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCTACAACTCAGGACATTTCCTATTTTAAAAGCCCGGGCTTCCGGTTCC11.1764705882352942No Hit
CTCAGTGCCTCGGTTTACTTTGTAACTTTATAAAGGAAACCGAGGCACTG11.1764705882352942No Hit
GCTGAAGGGTGTGGCCGGTAGATGACACTTGGTGAGGTAGTCTGCTCTTG11.1764705882352942No Hit
GTGCGTGGAGATAGGCTTTCTGTATAATGGATTTGAAGCCATTTCATAGC11.1764705882352942No Hit
CTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTCTAGAT11.1764705882352942No Hit
GATATACACAAAATTTTCTACTCCCACCCACCCCCCATGTCCACACCAAT11.1764705882352942No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG11.1764705882352942Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.1764705882352942No Hit
TATCAGCCATCATAGACAGCCCAGTGGGTGGTACCGTGTGGTTGGAGCTG11.1764705882352942No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTGCCCATGTACTCTGCGTTGA11.1764705882352942Clontech Universal Primer Mix Long (96% over 27bp)
CCCCCATTACAATAACCAGGAATTGTCTGCTTCAAACCTGGAAGCAGACA11.1764705882352942No Hit
GCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCT11.1764705882352942No Hit
ATATGAAGACTTAGAAGAGTAGCATGAGGACTGTCTCTTATACACATCTG11.1764705882352942No Hit
ACAGGAGACTGGACCTCTTCTCGCCTCTCCTATCACAACCCTAGTCCAAG11.1764705882352942No Hit
GGCGGGGGACCGGCTATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCCGT11.1764705882352942No Hit
GAATAAGTCCTAGGGGTGGGGGGTGTGGCAAGCCGGCCTGGATCCTGTCC11.1764705882352942No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA11.1764705882352942Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA11.1764705882352942Clontech SMART CDS Primer II A (100% over 26bp)
CATAAACCCTGCTTCATTCTCACAGTTGGTTATAAGCTGTCTTTCTGACC11.1764705882352942No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT11.1764705882352942Clontech SMART CDS Primer II A (100% over 26bp)
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATAACAATGCTTCCC11.1764705882352942No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)