FastQCFastQC Report
Tue 10 May 2022
EGAF00003627515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003627515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458639
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT13210.28802609459727585No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG10100.2202167717965546No Hit
GTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATA9710.2117133518954995No Hit
ATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGTGTATGAGTTGG9290.20255582277128636No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC8500.18533094656145685No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT8000.17442912617548878No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA7530.1641814150126788No Hit
GTACTACGTTGTAGCCCACTTCCACTATGTCCTATCAATAGGAGCTGTAT7470.16287319656636265No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC6640.14477617472565568No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6600.14390402909477826No Hit
GTACATGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG6560.1430318834639008No Hit
CTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAA6520.14215973783302335No Hit
GTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTT6080.13256613589337146No Hit
CCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGC5430.11839376939161302No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG5410.11795769657617428No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG5290.11534125968354196No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT5270.11490518686810323No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT5180.11294285919862898No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA5170.11272482279090963No Hit
GGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTT5060.11032642230599667No Hit
CCTTTAACCTCTCCGCCCTTATCACAACACAAGAACACCTCTGATTACTC5000.10901820385968049No Hit
TAGTGGAAGTGGGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAG4950.1079280218210837No Hit
ACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATAC4860.10596569415160945No Hit
TCATATAAGTAATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGT4860.10596569415160945No Hit
CATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCC4820.105093548520732No Hit
GGTGGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTAC4650.10138692958950285No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)