FastQCFastQC Report
Wed 27 Apr 2022
EGAF00003627528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003627528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences755888
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG18460.24421607433905554No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT16270.21524352814173528No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA12220.16166416188641705Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG11950.15809220413606248No Hit
CTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTG11900.15743073047858944No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA10810.14301060474567662No Hit
GGGTAAATACGGGTCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG10250.1356020997819783No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA10150.13427915246703215No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCCGTAAGTAGGAGAGTGATAT10030.1326916156890968No Hit
GTACATGGGATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAAT9980.13203014203162375No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT9730.12872277374425842Clontech Universal Primer Mix Long (96% over 26bp)
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC9400.12435704760493618No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC9230.12210803716952777No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC8980.11880066888216244No Hit
ACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATAC8780.11615477425227017No Hit
GAATATGATAGTGAAATGGATTTTGGCGTAGGTTTGGTCTAGGGTGTAGC8670.11469953220582943No Hit
TATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATATCATAG8480.11218593230743179No Hit
ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT8440.11165675338145334No Hit
GTATATAGGCTGAGCAAGGGGTGGTGAGGTTGATCGGGGTTTATCGATTA8400.11112757445547489No Hit
GTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCC8390.11099527972398028No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA8250.1091431534830557No Hit
CACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCAC8180.1082170903625934No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT8090.10702643777914188No Hit
ATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCC8080.10689414304764727No Hit
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT8040.1063649641216688No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG7800.10318989056579812No Hit
GTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCC7780.10292530110280887No Hit
CTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAAT7590.10041170120441123No Hit
GGGTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAAT7570.10014711174142202No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)