FastQCFastQC Report
Tue 10 May 2022
EGAF00003627537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003627537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44810
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCGTATTCTGCATCTCGTATG3010.6717250613702298No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGTATTCTGCATCGCGTATG2640.5891542066503013No Hit
ATACACATCTCCGAGCCCACGAGACTTCGTATTCTGCATCTCGTATGCCG2390.5333630885962954No Hit
ATACACATCTCCGAGCCCACGAGACTTCGTATTCTGCATCGCGTATGCCG2280.5088149966525329No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2200.490961838875251Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACACATCTCCGAGCCCACGAGACTTCGTATTCTGCATCTCGTATGCCGTC1920.42847578665476455No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC1560.3481365766569962No Hit
ACACATCTCCGAGCCCACGAGACTTCGTATTCTGCATCGCGTATGCCGTC1550.345904931934836No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1340.2990403927694711Clontech Universal Primer Mix Long (96% over 26bp)
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG1330.2968087480473109No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1290.28788216915866993No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA1210.2700290113813881No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA1190.2655657219370676No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT1130.25217585360410627No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1090.24324927471546529No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1060.2365543405489846Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG950.21200624860522205No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA940.20977460388306182No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG930.2075429591609016Clontech Universal Primer Mix Long (96% over 26bp)
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT920.20531131443874134No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA780.1740682883284981No Hit
GTACATGGTAGTTCAGCCAATAGATGCCTAGTACGCTGACGGCATTCAGA770.17183664360633788No Hit
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA750.1673733541620174No Hit
GAGTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGG720.16067841999553673No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT720.16067841999553673No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT700.15621513055121625No Hit
GCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTC700.15621513055121625No Hit
CATCTCCGAGCCCACGAGACTTCGTATTCTGCATCTCGTATGCCGTCTTC670.14952019638473554RNA PCR Primer, Index 18 (95% over 23bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG620.1383619727739344Clontech Universal Primer Mix Long (96% over 26bp)
GTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAG600.13389868332961394No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC600.13389868332961394Clontech SMART CDS Primer II A (96% over 26bp)
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT590.13166703860745368No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT590.13166703860745368No Hit
CACATCTCCGAGCCCACGAGACTTCGTATTCTGCATCTCGTATGCCGTCT590.13166703860745368TruSeq Adapter, Index 18 (95% over 21bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT570.12720374916313323Clontech SMART CDS Primer II A (100% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT570.12720374916313323Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC570.12720374916313323No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC570.12720374916313323Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT570.12720374916313323Clontech Universal Primer Mix Long (96% over 26bp)
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG550.12274045971881276No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA540.12050881499665253No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC540.12050881499665253No Hit
CGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTG530.1182771702744923No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT520.11604552555233208No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT520.11604552555233208No Hit
CATCTCCGAGCCCACGAGACTTCGTATTCTGCATCGCGTATGCCGTCTTC510.11381388083017184No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA510.11381388083017184No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT500.11158223610801161No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC500.11158223610801161No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG490.10935059138585136No Hit
CAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTA480.10711894666369114No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT480.10711894666369114Clontech SMART CDS Primer II A (100% over 26bp)
GTGTTAGATATTGAGGCTGCTTCGTGTCGGCACGAAGTGTCTTCCGATGC480.10711894666369114No Hit
GAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCG480.10711894666369114No Hit
ATTGTGAGTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCA470.10488730194153091No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.10265565721937067No Hit
GTACATGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATGCAC450.10042401249721045No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)