FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003627600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003627600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT228.57142857142857No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA114.285714285714285Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCGCGCCCCCACACCCCGAAGATACAAGCGCCCCGCTGGGCCCAGCCCC114.285714285714285No Hit
TGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG114.285714285714285Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA114.285714285714285Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCGCGCAAGCACACCCACAAAATACACCCCCCCCAATGGGCCGAGAACC114.285714285714285No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)