FastQCFastQC Report
Wed 27 Apr 2022
EGAF00003627694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003627694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTA22.272727272727273No Hit
GGGCAGCTGCTGCTATTAAGTTGCAAGCTCTACAGCTAGCTACATGACTG22.272727272727273No Hit
GCCCATCACCGTGACCAGCACGAAAAAGAAGACGAAGACCGAGAGCGCGG22.272727272727273No Hit
TCGCTGGACGGTACATTTCCCCGGGAGAACTCGTCCATTCGGTGGTGGCG22.272727272727273No Hit
AGATAAAGAATATTCGTGTAAGATGGAGTATCTGTATTTATTGCAGTGCT22.272727272727273No Hit
GGTCACGCCAACCCATGATGAAAAGCCAAAAATGCAACACAACGATAGCC22.272727272727273No Hit
GCCTAAGTGAGCTCAGGGATGCAGTGATGACTAAGGCAGTCTCTGCCTTC22.272727272727273No Hit
AGTATATTCAGCGACTTCCTTGGAAGCGCCTACGTTAGAATACTACAAAC22.272727272727273No Hit
CACTGCAGCAGTACCTGGCATGATGGGAGTGCCCCTGGGCACCTGCGGGC11.1363636363636365No Hit
CCATAATTCTATTCGATTACATTCGATGATGATTCCATTCATTTCCATCC11.1363636363636365No Hit
GAGCAGAAAAACTGAAAATTCTAAAAGTAAGAATGCCTCTCCTCCTCCAA11.1363636363636365No Hit
ATAAAAAGACTCATCAAGCTTATCGTCAGTTCTTCAACGTATCAGCAATC11.1363636363636365No Hit
NATATAAGACATATAGAAGAAAAAAAAAAGGTCTCAGTCCTCTTGTCTTC11.1363636363636365No Hit
GGTCCATTCTAAAAAAGAGCCCACATCTGCCCCAGACTTGACCTCTAATA11.1363636363636365No Hit
TAATATGACAGGCCGCTAAAGACGTTCTACTCCGCCATATGAGATAATAT11.1363636363636365No Hit
TCGCCGAAGAGCGCTTTGCCGAGCTCGACGCGGAATATGACCGGCTCGCT11.1363636363636365No Hit
ATTTGTAATAGCCCAACCCTGGAAATAACCCAAATGCTCATCAGCAGGTG11.1363636363636365No Hit
GGCCTGTATTGGGTGCGAGCGGGTGGTCATGCGGTGAGCATTGCGAAGCC11.1363636363636365No Hit
GTATCAACGCAGAGTACAGGGGAAGCAGTGGTATCAACGCAGAGTACATG11.1363636363636365Clontech Universal Primer Mix Long (96% over 28bp)
AGCATAGACTGGAAGAATCAGAAGGATTTCGAATTTGAGTATAAAATCGG11.1363636363636365No Hit
GGATGACGGCCTCGAACACACCCAGGCCCCCCGGGCTCTGGCTCAGCACG11.1363636363636365No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG11.1363636363636365Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTCAACTTCTAAACTTATACACTTCTAAACTTTAAACCTTAAAAGAAAA11.1363636363636365No Hit
GATACAGATTCCCATGACAGATGGAACCTTGGAACGCTACATTAAACAGG11.1363636363636365No Hit
GAATGGAAAGTCAAATTTCCTGAATTGCTATGTGTCTGGGTTTCATCCAT11.1363636363636365No Hit
CCATAGCACATAATCTACAAAACCGGAACTGGAAGGATTAGTGGAAATAG11.1363636363636365No Hit
TCCCAGGATCAAGGGATTCTCCAACCTCAGCATCCTGAGTAGCTAGGACT11.1363636363636365No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT11.1363636363636365No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA11.1363636363636365Clontech Universal Primer Mix Long (96% over 26bp)
GATGATAGTTGCATGGCCTTGGATATGCTAAGAACTACTGAAGTGTATGC11.1363636363636365No Hit
CCGCTATGAAGAGCTGGCTCGGATGCTGATGGGGCTGGACCTGCTGGTGT11.1363636363636365No Hit
CCGTCCTCTCCACGGACTGGAAAAGCTCCTTTCTGCTTGCTTCCTTCCTC11.1363636363636365No Hit
CCGAAGATGTGTACCGCCAGTTGCTCACGTCGGAACTCGACATCGGCATC11.1363636363636365No Hit
CTTGAAAATCTCCTTTTTGCGGTCTGTGATCTTGAGGAAACGGCCTTCCA11.1363636363636365No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG11.1363636363636365Clontech Universal Primer Mix Long (96% over 26bp)
GCTGCAGCTACCACCAGCACATTTTTCCTCAGCCAGCCCCCAATCTTCTC11.1363636363636365No Hit
GGACAACAATGGTACGGCAGAACCGAACTGGTTGGAAGTAAAACTTTCGG11.1363636363636365No Hit
ATCAGGATCCGTCCAGGCCAGTACATCAATATTGAAATTGCCATTGCCCG11.1363636363636365No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA11.1363636363636365Clontech Universal Primer Mix Long (95% over 21bp)
CAGTCATAGTTACTAATATTTGGAGGCAGTGGGAGATGATGGCCCCAAGG11.1363636363636365No Hit
TCGCAACAGGCGCACTGCTCGGCTCGCCGGCGCTCGTGCTGATCTCGCTC11.1363636363636365No Hit
GACACCGCACAGCTACAGGCGCTGATCAATGAGCGCGCGCTACACCACCA11.1363636363636365No Hit
TCCTGGAACACGGGCGTGTGTTTTATTTTCATCACCGAGGGATGGAGCTG11.1363636363636365No Hit
GTTGTTGAGCGTTGCGCTCTTGCCCGAGCCGGTGGGGCCGAACAGGAACA11.1363636363636365No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAGGGAAGCAGTGGT11.1363636363636365Clontech Universal Primer Mix Long (96% over 26bp)
CCTCTCCACCTAGCGAAACATAGGTGCTGCCATTTTCGGACAGCGGCATA11.1363636363636365No Hit
ATCAACGCAGAGTACATGGGCACCCGGCCCGGACACGGACAGGATTGACA11.1363636363636365No Hit
CATTTAAGGAAAAGGTTGAGGTGTTTCTCTCATGGGCTGTCTAAAAGGAG11.1363636363636365No Hit
CTGTAGTTCCAGCTACTCAGGAGGCTGAGGCAGGAAAATCACTTGAATCT11.1363636363636365No Hit
GAATATAGGTGTCAGTGGTCATGGGAGAACCTTTCGGATGAACCGTCCTG11.1363636363636365No Hit
GAATGGAATGGAATGGAATGAAATGGAATGGAATGGAATGCAATGGAATG11.1363636363636365No Hit
TAATGGATTTGTCCATTGTTGCCTATAATTACTATAGCAGGATTATTTTT11.1363636363636365No Hit
GATGACATATGTTCCTGTATTAGCTAGTCTCCATTCTGCCAGACTTGGCT11.1363636363636365No Hit
AGAGAGGATAGACTTGAGGTAGAAAACATCATGAACTAGAAAGTAAATGA11.1363636363636365No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11.1363636363636365Clontech Universal Primer Mix Long (96% over 26bp)
ACTTTCTGAGGATTCGTTGATGGCCTCTTATGAAGGAAGCCTTTACGGAG11.1363636363636365No Hit
GGCTGGCAGCTGGCGTGATCATCCGGCGTTGAACGGCGTCGTGCCGGTCG11.1363636363636365No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATCATTTA11.1363636363636365No Hit
CAATTATACCCTAGCCAACCCCTTAAACACCCCTCCCCACATCAAGCCCG11.1363636363636365No Hit
CACATGGGGTCGTACCACTGACCGAAACCTGCGGTAGCGAGCTTTTTCCA11.1363636363636365No Hit
CCCTGCTAAAGTCAAACCTGACAATTACCCGGATGCCGTTTTCATGGCAT11.1363636363636365No Hit
GCCATCTCCTGTGACCCTTCATTCTACAAATGAGGGAGCTGCGTGGGTAT11.1363636363636365No Hit
GTATATGTTTTGTTTTTTAAAAAAGCTTTGGAGTCTATACATTTTTTGTT11.1363636363636365No Hit
CCCCTGAAGAGCATCCCACCCTGCTCACGGAGGCACCCCTGAACCCCAAG11.1363636363636365No Hit
GGATACCCGCTGCTGCTCCCCTCCCGACAATTGAGAGGGATAATGAGTCG11.1363636363636365No Hit
AAAGACCACAGCAAGATTCTTTCATTCGTCTCCTCCTAGCCTGGGGGACC11.1363636363636365No Hit
CCTCTCTTCTGCGCAACTCACCATGATCGCCATCGGCAGTGCGATCGGCA11.1363636363636365No Hit
TAGCTGGAACGTACATTACCCACGTAGAACTCGTCCATAAGGTTGTGGCT11.1363636363636365No Hit
CCCTTATTGTTATTTTGTTAAAATTCCCACAATCTTTGATTTTTTTTGAC11.1363636363636365No Hit
ATGATGCCCTTGCCGCCGTCGGTGGTCTTCAGGGGCACCATCTCGCTGCC11.1363636363636365No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA11.1363636363636365Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGATAGGGCTTGACTCCAAATATAAGTACATCAACAAAAAATTTGCGGAA11.1363636363636365No Hit
GTGCGGGACTCTGGAGATGGTGTCACCCACAATGTCCCCATCTATGAGGG11.1363636363636365No Hit
AGCTGGAGGAGGAGGGGCTGACCGGGCAGGTGCAGCTGCGGATCGTCGAG11.1363636363636365No Hit
TTCCACTCCAGCTTAAGGTGAAAATCATCGAACGATTCTTTTGTCACCAG11.1363636363636365No Hit
CATATAAGACATATAGAAGAAAAAAAAAAGGTCTCAGTCCTCTTGTCTTC11.1363636363636365No Hit
CACCCAATACGACCCCTCACTTTATGAAGCCAACAGCCGATTTCTTTATC11.1363636363636365No Hit
CTTCTCATCGTCGGTGAGCCATTTGGCCTTTTCAGGCCGATCCGCCAGCA11.1363636363636365No Hit
ATTTCATGTAAGGCATCAACCACTGTAATTTTTGCTGATGCTGAAGCCTG11.1363636363636365No Hit
AGATTGTTATGAGTCAACAGCTATAATTTAGGTGGTGGTGACAGTGACTT11.1363636363636365No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)