FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003627757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003627757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCACTCGCCGCGGATCACCCGTTGCACGATTTTGCGCGAGAAAGAGAC22.9850746268656714No Hit
TTCGTCTTCCTGGGCATCGGGTTCGTCCAGCCGTCCAAGGGCTTCGACCG22.9850746268656714No Hit
ACCTGATGTCGCTGTCCGCCCACCGACGGTCAGGACTACGCATCACCCCG22.9850746268656714No Hit
GGTTGAAAGGGCTGGTGGCCTTCACGAACCCGGAAGGGCTGGCCGCGCTT22.9850746268656714No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT22.9850746268656714No Hit
GTAGACAGTCCCACCCTCACACGATTCTTTACCTTTCACTTCATCTTGCC11.4925373134328357No Hit
GAAGCAGTGGTATCAACGAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAA11.4925373134328357No Hit
GGAAGCAATGGTATCAACGCAGAGTACATGGGCTGTCTCTTATACACATC11.4925373134328357Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAACACT11.4925373134328357No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG11.4925373134328357No Hit
GCGGGGACGGGCGTGGGTGCGGCGTTGGCGCTCACTGGGCGTTCCCCCCT11.4925373134328357No Hit
GTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAA11.4925373134328357No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG11.4925373134328357Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAACACTGAAAGTTTACAAGCAACCGCGTCCTAATGGATATAACATTGA11.4925373134328357No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTGTTA11.4925373134328357No Hit
GTCCAAATGATTGAAATATATAACAAACAAGTTTAGGATCTACTTGTAAA11.4925373134328357No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG11.4925373134328357Clontech SMARTer II A Oligonucleotide (100% over 23bp)
ATCCGAGAGCGGCTGGAGGCCGGCTTCGGCGAGCGCCCGGACGGCGACGC11.4925373134328357No Hit
GGTATCAACGCAGAGTACATGGGTGACGTTACCCGCAGAAAAAAAAAAAA11.4925373134328357No Hit
CCCGGAGGTCCGCNGCGGTCTCCACGACGGTCGGCGTGACCACGGCGGTC11.4925373134328357No Hit
CACTACAAGAAACCCAGCACTTTTTTTTTTTTTGAGACAGAGTCTTGCTC11.4925373134328357No Hit
ATCCTCGTGCTGGAGACGCGCGAGAACGCCGAGAAGCGCGGCGCCAAGAT11.4925373134328357No Hit
GGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTGGTTGTCCTC11.4925373134328357No Hit
GCCCGGTACTCGGAAGCCGCCGCGTTGGCCCGCTGCTCGGCCTCGCCGGC11.4925373134328357No Hit
GATGTATGTGCTGCAGCCGCCGGGCTGGTACTGGGGCGACGTGCCGGCGT11.4925373134328357No Hit
ATCCGACGCTGTCGCAGCGCCGTTATCTCCGGTATATCGAGGGGTTCTAA11.4925373134328357No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA11.4925373134328357Clontech Universal Primer Mix Long (95% over 21bp)
GAACTGGCGATCAACCCCGACGGTTCGCCGATCCGCATCGAGCAGCAGCG11.4925373134328357No Hit
CCCTTCATCATCACCGACCCGATCCAGGCCCCCGAGCAGGCCGCGCGCTT11.4925373134328357No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11.4925373134328357No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGATGTCTATTATAAACAT11.4925373134328357No Hit
GAACAGAGGTAAGATTAAGAACAGATGGACCGGCTGGCTTTAGAGCTATT11.4925373134328357No Hit
CCCTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAA11.4925373134328357No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTGAAAACACTCC11.4925373134328357No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA11.4925373134328357No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC11.4925373134328357Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGGCTGACATGGTGGCACCTGTTCGGCTTCGCCGCGGTAATGAGCGGCA11.4925373134328357No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA11.4925373134328357No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11.4925373134328357Clontech Universal Primer Mix Long (96% over 26bp)
TTTTTTTCGTCGGCAGCGTCAGATGTGAAAAAAAAAAAAAAAAAAAAAAA11.4925373134328357No Hit
CTGCAGGGTGTCGCCGACCAACGGCACCTTGCGCAGGCGCTGGCCCGCCT11.4925373134328357No Hit
CGCTACTGGGCTTCGAGTTGCCCGGGCCCTTCTCGTTCGAAACGGTCTTC11.4925373134328357No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAGTTTAAACC11.4925373134328357No Hit
GAACCGCAGTGGGGATTAAGCCACGTGGATGTTTTTGCCTCCATCTCAGC11.4925373134328357No Hit
CCCTCACACGATTCTTTACCTTTCACTTCATCTTGCCCTTCATTATTGCA11.4925373134328357No Hit
GGCGGGACGTGAGCCGGCCCGCGATCCTGGTGCTGCCTGGCATCGCCGTC11.4925373134328357No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA11.4925373134328357No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT11.4925373134328357Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC11.4925373134328357Clontech Universal Primer Mix Long (96% over 26bp)
TGTGTATAAGAGACAGATGTGGCGATTGTGCGTATCGAGCAGCTTTATCC11.4925373134328357No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTCGTTGATAACCAAGGGTCCCC11.4925373134328357No Hit
CCTCCATATATCCAAACAACAAAGCATAATATTTCGCCCACTAAGCCAAT11.4925373134328357No Hit
GTGGGCTACACCAAGGACACGCCCGCGCCTCTGTCTCTTATACACATCTC11.4925373134328357No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTA11.4925373134328357No Hit
GCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAG11.4925373134328357No Hit
CGCCTATGTGGCGGTGAATCAGCTGGCGTTGGCGGGACGATGACAGGTTA11.4925373134328357No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA11.4925373134328357Clontech Universal Primer Mix Long (95% over 21bp)
ACTTGTATGATTATTTTACAAGCTAATAAAATTGAACGTTCTTTTGCAGG11.4925373134328357No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACACAAACTGTCTCTT11.4925373134328357No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTG11.4925373134328357No Hit
GAGCAGCACCAACCCGCCAGCGTTGACACGCGGGGTGACCTTTAGGATCA11.4925373134328357No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTTAACACTGCTTC11.4925373134328357No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)