FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003627758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003627758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGCAGCCGATGCCAAGCCGCAACCGAACTTGCTCCGGCTTTCCGTCGG22.9850746268656714No Hit
GGTCGGGGTGATGCGTAGTCCTGACCGTCGGTGGGCGGACAGCGACATCA22.9850746268656714No Hit
GAAGCGGCTGGGGTGCGAGGCACGCTCAAGCACTTTCCCGGCCTGGCGGA22.9850746268656714No Hit
ATGTTCCGGTAGGGCAGCACGACGACGTCCGCGGCGACGATCCACCGGTC22.9850746268656714No Hit
GTATCAACGCAGAGTACATGGGAAGCAGCGGTATCAACGCAAAAAAAAAA22.9850746268656714No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA22.9850746268656714No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.4925373134328357No Hit
CTTTTGCAGCTTGGGCAAGCTTGTCATTCACCGCCACATAGGCGGCTTAG11.4925373134328357No Hit
ATCCTGTTCGACGCGGCGATCGTCACGTGGCTGGCCTTCTGGTGGAACGT11.4925373134328357No Hit
CAACTGGCTCAAGCAAGTAAGCTTTGGTCGGACGAGTCGCTGGTTTTGGC11.4925373134328357No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA11.4925373134328357No Hit
AGCGTGCGCAGCGCGGCCGTGTCCTGGCGGGTGCTGCCGTCGATGCGCAC11.4925373134328357No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC11.4925373134328357Clontech SMART CDS Primer II A (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTATAACACT11.4925373134328357No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGT11.4925373134328357No Hit
CGGCACTTTTCATTTTTTCATGTAGGCCCAAGTTTTTGACATCACTTTCC11.4925373134328357No Hit
GCTCGACCTGCTCGACGACAGCCGCGACGGGGCGGGCTCGCGGTGACCGC11.4925373134328357No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG11.4925373134328357Clontech Universal Primer Mix Long (96% over 26bp)
AATAGGAGGTGGAGTGCTGCTAGGGCTGCAATAATGAAGGGCAAGATGAA11.4925373134328357No Hit
GCCAAACCCCCCGAAGCGAGGGCGGCGAATGCGGCGTCGGCGTCCTCGAC11.4925373134328357No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT11.4925373134328357No Hit
GACCAGAACGGACAGGAATTCACCCGTCTCCGGCGTGATGTTGAGCATGC11.4925373134328357No Hit
CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA11.4925373134328357Clontech Universal Primer Mix Long (95% over 22bp)
GTCCTGAAAGCAAGCCCTGTTTAAATCGGAAAAATACACAGGCAGCGTGG11.4925373134328357No Hit
CTTCATTTCCATCAACCCGGTGCGCACCGGCTATTCGGCCATCGCCGACG11.4925373134328357No Hit
AGGCGCGGGCGTGTCCTTGGTGTAGCCCACCTGTCTCTTATACACATCTG11.4925373134328357No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT11.4925373134328357No Hit
TACTGTGGCCGAGCAGCACGACGAACCGGGTGCCGAGACGCTGCTCCATC11.4925373134328357No Hit
AGCTGGTGGTGAGGAACAGGATCACGCAGCCCGCCAACCAGAACGCGGTG11.4925373134328357No Hit
CACACACACACACACACACACACACACACACACCTGTCTCTTATACACAT11.4925373134328357No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11.4925373134328357Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAC11.4925373134328357No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAGT11.4925373134328357No Hit
CCCATGTACTCTGCGTTGATACCATTGCTTCCCTGTCTCTTATACACATC11.4925373134328357Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCATTAGAATCACAGCGAGTTAGAATAAATTTGTGTAGCCGAGCCCCGCC11.4925373134328357No Hit
GAACCAATGTGAAGGTCGTGTCGGCGCCCAAGCTGATCACGCTCAACAAT11.4925373134328357No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGTAAAA11.4925373134328357No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAAACTGC11.4925373134328357No Hit
GGGGGAACGCCCAGTGAGCGCCAACGCCGCACCCACGCCCGTCCCCGCCT11.4925373134328357No Hit
GGTCTGCGGCTAGGAGTCAATAAAGTGATTGGCTTAGTGGGCGAAATATT11.4925373134328357No Hit
GGTGTTTAAGGGGTTGGCTAGGGTATAATTGTCTGGGTCGCCTAGGAGGT11.4925373134328357No Hit
CACATGTACAGTCAAGATACTATGAGATCGGTTGCTATACTCATTAAGTG11.4925373134328357No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT11.4925373134328357Clontech Universal Primer Mix Long (96% over 26bp)
GAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAA11.4925373134328357No Hit
TTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTA11.4925373134328357No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG11.4925373134328357Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC11.4925373134328357Clontech Universal Primer Mix Long (96% over 26bp)
GATATGTCCTGATCCGCACCACTGGTCTTTATCTCTCCTGAGTTTGTCCT11.4925373134328357No Hit
GTGCCACGGCGTGGCATGGCCGCGGTCGGCGGCGTGCTGGCACATCGGCG11.4925373134328357No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGT11.4925373134328357No Hit
GTTTAGAACCCCTCGATATACCGGAGATAACGGCGCTGCGACAGCGTCGG11.4925373134328357No Hit
TCTCCGTACGACGTAAGTCATCAAAGACATGAAGCATCTCATCGTAGATG11.4925373134328357No Hit
AACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA11.4925373134328357No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA11.4925373134328357Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCACGTACACGATGTCCTCGGGCGTGATGCCCGCATCCTCCATCGCCCGC11.4925373134328357No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA11.4925373134328357Clontech Universal Primer Mix Long (95% over 21bp)
CCATATATCCAAACAACAAAGCATAATATTTCGCCCACTAAGCCAATCAC11.4925373134328357No Hit
CTTCTTCCTCGTGCTGATGCTGCCGGGCGCGGGCGACGAGCTGCAGGGCA11.4925373134328357No Hit
TTTGTGTTGATACCACTGCCCCATGTACTCTGCGTTGATACCTGTCTCTT11.4925373134328357No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTT11.4925373134328357No Hit
GTACATGGGAAGCAGTGGTATCACGCAAAAAAAAAAAAAAAAAAAAAAAA11.4925373134328357No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)