FastQCFastQC Report
Tue 10 May 2022
EGAF00003627907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003627907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences692096
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGAAGAAGAGATGTTGTCCTGACACTTGTGGCATCAAATGCCTG24030.3472061679304605No Hit
GTACATGGGAGAGTCACTCCTGCCTTCACCATGAAGTCCAGCGGCCTCTT23630.34142662289624565No Hit
GATCAGCACCATGAAGCTTCTCACGGGCCTGGTTTTCTGCTCCTTGGTCC23240.33579156648788605No Hit
GAGCAGGACTCCAGAGCCTCCTCCATATGGCAGGAATCAAGCTTTCACAG18550.26802640096171626No Hit
CTCCTGCCTTCACCATGAAGTCCAGCGGCCTCTTCCCCTTCCTGGTGCTG15400.2225124838172739No Hit
GGACCACACAGAGCAGGACTCCAGAGCCTCCTCCATATGGCAGGAATCAA15260.2204896430552987No Hit
GCTCTGGAAAGTCCTTCAAAGCTGGAGTCTGTCCTCCTAAGAAATCTGCC13570.1960710652857407No Hit
TCCTGGTGCTGCTTGCCCTGGGAACTCTGGCACCTTGGGCTGTGGAAGGC13270.1917364065100795No Hit
CTACAGCACAGATCAGCACCATGAAGCTTCTCACGGGCCTGGTTTTCTGC13020.1881241908636952No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG12850.18566788422415387No Hit
GTGCCAAGCCTTTCCCCAAAGGAGGATATCAGTGGTGGAGCCAAGTCTCA12250.1769985666728315No Hit
CTCTGGCACCTTGGGCTGTGGAAGGCTCTGGAAAGTCCTTCAAAGCTGGA12140.1754091917884224No Hit
CTCCAGAGCCTCCTCCATATGGCAGGAATCAAGCTTTCACAGGGGAAACG11790.17035208988348438No Hit
GACCTGGACCACACAGAGCAGGACTCCAGAGCCTCCTCCATATGGCAGGA11420.1650060107268356No Hit
GCCTTAGATACAAGAAACCTGAGTGCCAGAGTGACTGGCAGTGTCCAGGG11090.16023788607360828No Hit
ACCTTGGGCTGTGGAAGGCTCTGGAAAGTCCTTCAAAGCTGGAGTCTGTC10810.15619220454965785No Hit
TCTTGAAAGCCTGCTGTGTGCCAAGCCTTTCCCCAAAGGAGGATATCAGT10460.15113510264471983No Hit
CCCCAAAGGAGGATATCAGTGGTGGAGCCAAGTCTCAGGGTGGAAAGGAC10280.1485343073793231No Hit
ACACAGAGCAGGACTCCAGAGCCTCCTCCATATGGCAGGAATCAAGCTTT10170.146944932494914No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG10080.14564453486221565No Hit
CCATATGGCAGGAATCAAGCTTTCACAGGGGAAACGCAGGATTTCCCACA9870.14261027371925283No Hit
GTACATGGGAGGGACCCGCAGCTCAGCTACAGCACAGATCAGCACCATGA9650.13943152395043462No Hit
CCTCCATATGGCAGGAATCAAGCTTTCACAGGGGAAACGCAGGATTTCCC9650.13943152395043462No Hit
AGCCTGCTGTGTGCCAAGCCTTTCCCCAAAGGAGGATATCAGTGGTGGAG9500.13726419456260402No Hit
CCTAAGAAATCTGCCCAGTGCCTTAGATACAAGAAACCTGAGTGCCAGAG9380.13553033105233955No Hit
GTGTACCCTCTCCCCGCTTTGTATCCCTGCCCTGAGGGCCTCACAGCCAG9020.13032874052154614No Hit
CGCTTGCACTGGCCATCCATCTCACAGAAATTGGGGGGGTTAAGCATCAA8910.12873936563713703No Hit
GAAATAGGCTCGTTTATTTATTCATTGATCAACTGGCACTTCTTGAAAGC8800.12714999075272793No Hit
GAGAAATAGGCTCGTTTATTTATTCATTGATCAACTGGCACTTCTTGAAA8370.12093697984094692No Hit
GTGCCAGAGTGACTGGCAGTGTCCAGGGAAGAAGAGATGTTGTCCTGACA8300.11992555945995931No Hit
GTGGAAGGCTCTGGAAAGTCCTTCAAAGCTGGAGTCTGTCCTCCTAAGAA8090.11689129831699649No Hit
TATCAGTGGTGGAGCCAAGTCTCAGGGTGGAAAGGACCTGGACCACACAG8040.11616885518771962No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTTACCAC7980.1153019234325874No Hit
GGATATCAGTGGTGGAGCCAAGTCTCAGGGTGGAAAGGACCTGGACCACA7820.11299010541890142No Hit
CTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTG7780.11241215091547993No Hit
GTCCTGACACTTGTGGCATCAAATGCCTGGATCCTGTTGACACCCCAAAC7610.1099558442759386No Hit
GTCTCAGGGTGGAAAGGACCTGGACCACACAGAGCAGGACTCCAGAGCCT7420.10721056038468652No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)