FastQCFastQC Report
Wed 27 Apr 2022
EGAF00003627908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003627908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences692096
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGGAAGAAGAGATGTTGTCCTGACACTTGTGGCATCAAATGCCTG23970.34633923617532825No Hit
GTACATGGGAGAGTCACTCCTGCCTTCACCATGAAGTCCAGCGGCCTCTT21680.31325134085444795No Hit
GATCAGCACCATGAAGCTTCTCACGGGCCTGGTTTTCTGCTCCTTGGTCC21570.3116619659700388No Hit
GAGCAGGACTCCAGAGCCTCCTCCATATGGCAGGAATCAAGCTTTCACAG17710.255889356389865No Hit
CTCCTGCCTTCACCATGAAGTCCAGCGGCCTCTTCCCCTTCCTGGTGCTG14610.21109788237469945No Hit
GGACCACACAGAGCAGGACTCCAGAGCCTCCTCCATATGGCAGGAATCAA14030.20271754207508785No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG13560.19592657665988533No Hit
GCTCTGGAAAGTCCTTCAAAGCTGGAGTCTGTCCTCCTAAGAAATCTGCC13550.19578208803402997No Hit
CTCCAGAGCCTCCTCCATATGGCAGGAATCAAGCTTTCACAGGGGAAACG12890.18624583872757536No Hit
TCCTGGTGCTGCTTGCCCTGGGAACTCTGGCACCTTGGGCTGTGGAAGGC12720.18378953208803403No Hit
CTACAGCACAGATCAGCACCATGAAGCTTCTCACGGGCCTGGTTTTCTGC12210.17642061216941002No Hit
GCCTTAGATACAAGAAACCTGAGTGCCAGAGTGACTGGCAGTGTCCAGGG12170.17584265766598856No Hit
GTGCCAAGCCTTTCCCCAAAGGAGGATATCAGTGGTGGAGCCAAGTCTCA11240.16240521546143888No Hit
CTCTGGCACCTTGGGCTGTGGAAGGCTCTGGAAAGTCCTTCAAAGCTGGA11010.1590819770667653No Hit
ACCTTGGGCTGTGGAAGGCTCTGGAAAGTCCTTCAAAGCTGGAGTCTGTC10660.15402487516182725No Hit
GACCTGGACCACACAGAGCAGGACTCCAGAGCCTCCTCCATATGGCAGGA10660.15402487516182725No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG10360.1496902163861661No Hit
GTACATGGGAGGGACCCGCAGCTCAGCTACAGCACAGATCAGCACCATGA10000.14448862585537267No Hit
CCTAAGAAATCTGCCCAGTGCCTTAGATACAAGAAACCTGAGTGCCAGAG9870.14261027371925283No Hit
CCTCCATATGGCAGGAATCAAGCTTTCACAGGGGAAACGCAGGATTTCCC9790.14145436471240982No Hit
ACACAGAGCAGGACTCCAGAGCCTCCTCCATATGGCAGGAATCAAGCTTT9750.14087641020898836No Hit
TCTTGAAAGCCTGCTGTGTGCCAAGCCTTTCCCCAAAGGAGGATATCAGT9730.1405874329572776No Hit
GTGTACCCTCTCCCCGCTTTGTATCCCTGCCCTGAGGGCCTCACAGCCAG9610.13885356944701313No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTTACCAC9560.13813112631773625No Hit
CCCCAAAGGAGGATATCAGTGGTGGAGCCAAGTCTCAGGGTGGAAAGGAC9510.1374086831884594No Hit
GAGAAATAGGCTCGTTTATTTATTCATTGATCAACTGGCACTTCTTGAAA9400.1358193083040503No Hit
CCATATGGCAGGAATCAAGCTTTCACAGGGGAAACGCAGGATTTCCCACA9010.13018425189569077No Hit
AGCCTGCTGTGTGCCAAGCCTTTCCCCAAAGGAGGATATCAGTGGTGGAG8650.12498266136489736No Hit
CGCTTGCACTGGCCATCCATCTCACAGAAATTGGGGGGGTTAAGCATCAA8500.12281533197706677No Hit
GTGCCAGAGTGACTGGCAGTGTCCAGGGAAGAAGAGATGTTGTCCTGACA8160.11790271869798409No Hit
GTCCTGACACTTGTGGCATCAAATGCCTGGATCCTGTTGACACCCCAAAC8080.11674680969114111No Hit
GTGGAAGGCTCTGGAAAGTCCTTCAAAGCTGGAGTCTGTCCTCCTAAGAA7940.11472396892916589No Hit
CTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTG7760.11212317366376919No Hit
GAAATAGGCTCGTTTATTTATTCATTGATCAACTGGCACTTCTTGAAAGC7710.11140073053449231No Hit
TATCAGTGGTGGAGCCAAGTCTCAGGGTGGAAAGGACCTGGACCACACAG7370.10648811725540966No Hit
GACTGGCAGTGTCCAGGGAAGAAGAGATGTTGTCCTGACACTTGTGGCAT7300.10547669687442204No Hit
CGTTTATTTATTCATTGATCAACTGGCACTTCTTGAAAGCCTGCTGTGTG7010.10128652672461624No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)