FastQCFastQC Report
Sat 23 Apr 2022
EGAF00003627992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003627992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98
Sequences flagged as poor quality0
Sequence length100
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCGGGCGCGCTGCTGGCGCACGGCTACCAGGCCAACCGCCTGCTCGCC33.061224489795918No Hit
GTTTCGGCCCTTCACCATGAGCCACTACCGGCAGGCCGTCCACACGCTGA33.061224489795918No Hit
AGCGAGACGCGCAATAGCGAAAGCCGCATCCGGCATGTCAGGGGACACAC33.061224489795918No Hit
GTGATGACCGGATCGCGCAGGTCGGCCGGCAGCTCGGCGCGGATGCGGGC22.0408163265306123No Hit
CAGAAAAGGAAGGGAGACAGAGACAACCTGGGGAAGAATGATGACACTTG22.0408163265306123No Hit
GAGCAGAAGAGCTAATGTGATAAAAGCAGTGTGAGATGTGAGTGACACTT22.0408163265306123No Hit
ACGCAGGATTTCCGATTGACGCGAGCCTTCGGCTTCGAGAATCTGCGCGC22.0408163265306123No Hit
CACCTGAGAGCCGTAATTAAAGATCGTCAGCGGTAGGCCGAAAACGTCGA22.0408163265306123No Hit
CCATGCTGCCGCCCGCCGCACGGGCGATGACGGTGCTCGCCAATGAAGAA22.0408163265306123No Hit
CATCAGTTGAGGATGGTGGCTACGGGTTGGATCCTGAACGTCTATGGGTC22.0408163265306123No Hit
GTCGTGAGTGCAGAGGCTTTGGGGGTTCTCCTGCTCTCGACCGGGATCGG22.0408163265306123No Hit
GTCGTCACCCTCGGCCTGGATCATGCGGTCCAGCTGCTCGGCGAGATGGC22.0408163265306123No Hit
GGAAGGGTGCGCCGAGTAGACCGGTGATGACGCCGATCGGCAGCTCCGCC22.0408163265306123No Hit
CATGCGCAGGTTAAGGCAGCATGGCAGCAGTCGGATTGACGTCCGAATCG22.0408163265306123No Hit
ACCACCGTCGGCGGCACCTTCGCCAGGAACGCCGCAATCGCATCGGCCGG11.0204081632653061No Hit
CATATCTGTCTTTCATACTTGTCCACTTACAAACTTTTTCAAGTAAATTC11.0204081632653061No Hit
GCATGATGGAGCGCGATGACTTCACCAAGCGCTACAAGGCCGGCGTGCCG11.0204081632653061No Hit
CGCTTGCAGAACGCATCCAGGTGGCCGACCTGGTGCTGGATCTGACTCGC11.0204081632653061No Hit
CTCACCTTGCATATGAAGGCTCTCTCACGATTACAGCCCAAATAGAAACT11.0204081632653061No Hit
GTAGGGGCCTGGCTGGAGTCACATTTTAGGAAAATCACTCCAGTGGCAAA11.0204081632653061No Hit
CGAATGTAATCATCTTCGAATTGAAAACAAAGCAATCATTAAATGTACTC11.0204081632653061No Hit
AGACGGTGAAGGGCTACACCATGCCGGTGTACCCCTCGCATCGGAACTGC11.0204081632653061No Hit
CCGCAGGGCAGGCGACGCGCTCGCAGCAGTGGGCAGACAGGAGCATCACT11.0204081632653061No Hit
GTAGAGCGCGTACGACCCGAGCATTTCTGCGGCATGCGCGAATGCCCAGA11.0204081632653061No Hit
CTGCTGCTAGTCCGGTAGCAACCCCGCTACCCGCTGCCCCCGCTGCTAAC11.0204081632653061No Hit
GCAGAGGCTGCACCTTCCTAAGCTGATGAAGAACTTCCTAGAATGTCCCC11.0204081632653061No Hit
GCGCCAGACCCGACTCTTCCACGTGGATGGCGACGCCACCGCGCTCCTCA11.0204081632653061No Hit
GCTGCGCGCCGTGCCCAGACGCAGGTCGGAAGACATCGCCAGGATGGCGC11.0204081632653061No Hit
GCTGCGCGCCGTGCACAGACGCAGGTCGGAAGACATCGCCAGGATGGCGC11.0204081632653061No Hit
GCCGGCCACCTTGTCGGTGCCCAGCGCATTGGCCGTAACGACCTTTCCAC11.0204081632653061No Hit
GTTTTGTCGCTGAACAGATCGAGGCCGGCAGCGGCATCATCGCTCTTGAG11.0204081632653061No Hit
ACCGTGCTGACCGGCGAGAGCGATGCCGCGGCGCGAGTAGAGGGTTGCGA11.0204081632653061No Hit
GATCAGCAGGTCTTCCTTCTCGTACACCAGTTGCTGCCGGTCGTATTCGG11.0204081632653061No Hit
CCAGCTCGGCCGAGGGCGGGTTGCTCATGACGGACGCGCCGATCTCTGCA11.0204081632653061No Hit
AGAGCGCCTGCACGCTCGCCAGCACCTGCGGCACCATCATCGCGGCCGAC11.0204081632653061No Hit
TCATTCGGGGGTCTTTCGATTCTCCTGCAGCAATGCCGATGAGCTCGGTG11.0204081632653061No Hit
ATTATGCAGGGCAGACAATTAGAGAACAAAATGATCTGTCATAAAGGGCT11.0204081632653061No Hit
TTTTATACCTGTTTTCCTCCTTCTCTTATTCCATTTAGTTTTTCAATTCA11.0204081632653061No Hit
CCAGACCAACATCCTGGCGCTCAACGCCGCCGTCGAGGCCGCCCGCGCCG11.0204081632653061No Hit
GCTTTACGGCAACCTCGCCGAGCAAGGTTGTATCGTGAAGACCGCGGGCG11.0204081632653061No Hit
ATGTGATGGGTTTCGTGACTTTAAGATACGGTTGGCCCAGAACCTTGCGG11.0204081632653061No Hit
CCAGCAGGCTGGCAAAGAAGAGGACACACATTGCGGGAAACGTTGAGTGC11.0204081632653061No Hit
GTAATGGAATGCAGTGGTATGGAATGGAGTCAACCCGAGTGGAATGGAAT11.0204081632653061No Hit
CCCGGGCCTGGCCCTTTAAGTGGAGGTTTTGGTTACACCTGCAAACATCA11.0204081632653061No Hit
GGCCGCGCCCGGCGACCCGGCGCTCGCCCGCCTGGCGCCGTCGCCCGGAC11.0204081632653061No Hit
TCCGGAAGGAGGCCGCCCTTTTTGTTTACTCCACCTCCAGCACCAGCTTC11.0204081632653061No Hit
AAGCCCCTCTGAGGGAATCCTGGCATGCTTTGGTCTTTGGTTTGGAAAAG11.0204081632653061No Hit
ATCTCCTTCAGCTGCCCTGCCCTGATCTACCACCTCCAACAGCTGTGGTG11.0204081632653061No Hit
TCGTCTTGTCATTCACGCCAGCGAAGACATTGGAATGGCGGACCCTACGG11.0204081632653061No Hit
ATGTGCGCACTATTCCGCACGCTATCCGTCATGGTGCCGTCATCGGTGCC11.0204081632653061No Hit
CGCTCGAGCAGTTGTCGCCGATCAGGATCTCGTAGCTGAATCGTAGTCCA11.0204081632653061No Hit
CACCTGCACATGCGCATCGACACGGTTTCCGGATGCGCACACCGTCGCGT11.0204081632653061No Hit
GGCTACGGCTTTGCTCATCCTCGCACAGGCCTGGTTGCTGTCCCGTGGTG11.0204081632653061No Hit
CCTCAACCCGTTGGGTGTGCCCTCCCGAATGAACGTCGGGCAGGTTCTTG11.0204081632653061No Hit
GCTGGCGGGCGCTGCGCACGCCGGCGCGTTCGTCCAGGGCAAGCGTGGTC11.0204081632653061No Hit
CTACTTCATTGCGCTGCGTTTCGTTCATTTGTCGTTCTCCGACCATGCCT11.0204081632653061No Hit
ATCGACGGCCGTATCCATGCGCTGCTTTTGCCATGTCTCACCGCCATCGC11.0204081632653061No Hit
AAATTTAAGTTCCGGCAGTCTTACGCAATAAGGCGCTAAGGAGACCTTAA11.0204081632653061No Hit
TTTGCACGTTGTTCATGATCAAAAGGGTTGCGCACGTCGCACTTTGCGAT11.0204081632653061No Hit
ATGCTCTATTTCGCGGCCAGGTTGTTTGCCGTGCGCCTCGTGGGGCATGG11.0204081632653061No Hit
GATACGATCCATACCAGTCCTTGTTCTTCGGTGTCGAGGCGAATCAGGCG11.0204081632653061No Hit
CAACCAGCGTTACACCCAACATCGTCAGCAAGGTAATCTGACTGCCAAAG11.0204081632653061No Hit
GAGCAGGGGCGCGACGTGCGGCCCTCGGTGGCGACCATCGAGGCGATCGG11.0204081632653061No Hit
AACCTGATCGAGGCATTCACTAACGACATGGACATCCACACGAAAACCGC11.0204081632653061No Hit
CTTTGGTGACAGAGTCTTGCCATGTTGCCCAGGTTGGTCTTGAACTCCTT11.0204081632653061No Hit
GTCCGTGGGGATGGCCAGGCGTTTGGCGATCGTGTCCGCCGTCTTGAAGC11.0204081632653061No Hit
CTCCCGGCCGTGGGAGGCCAGCTTGTGCCAGTCCGTGCCCCGGTCTGGCT11.0204081632653061No Hit
CAATCACATCGCGCGATAACGGCTTTGTCAAAGAGCTACGCCGCCTGGCG11.0204081632653061No Hit
GATCTCGATACCGCCGCGAAACAGGCCGCGGTCGATCCGGCTGCACAACT11.0204081632653061No Hit
GCCGAACATCCGGGTGCCGATGCTGGTGATTGGCGGCACACACGATATCT11.0204081632653061No Hit
AGACGGTCGCACGCGAGTGGCTGAAAAAGGCGGGTGTGGCAGAGCTGCAA11.0204081632653061No Hit
CCGTCCTACTTCCGCAACTTTCATTACGAGGTGCTGTCCGACTGGGGCCC11.0204081632653061No Hit
GCGTAGCGGAAGGTTAAGGGGGGAGTCCTTGACCTGTCAACCGCTACGCA11.0204081632653061No Hit
GCTAACCCGGCGATGTGTGCTCCGTTCGCCATCGACATCCCAAACAAATC11.0204081632653061No Hit
AACCTCTCCCTGTTTCCCTGTGACACCCCGATCCTACCCTCTCCAAATTC11.0204081632653061No Hit
TGTCCAAAGCCGTCGTCGTCGCCACCGGCATCACCGCCACCGGCCAACGA11.0204081632653061No Hit
GAAACATTCTGAGAATGCTTCTGTCTAGGTTTGATGTGAAGATATACCCG11.0204081632653061No Hit
CCTGTGGACGTCCTGTGTTCTCCAGACGCAGAGAACCCTCATCAACCGAG11.0204081632653061No Hit
GGTTTAAAGTCTGTTTTATCAGAGACTAGGATTGCAACCCCTGCTTTTTT11.0204081632653061No Hit
TTACAGTACAGTTCAATTCTCAAATATTTTGCTCTGCCTTATTTGCTCTG11.0204081632653061No Hit
ATGCACACCGCTGAAGCCACGCAGAACAAGGCCGACGCCCAGGGTGGCGT11.0204081632653061No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCTT50.094.09
CGGGGGT50.094.06
CACCGAA50.094.094
CATTCGG50.094.02
GGGGGTC50.094.07
ATTCGGG50.094.03
GGGGTCT50.094.08
TCATTCG50.094.01
TTCGGGG50.094.04
TCGGGGG50.094.05
Produced by FastQC (version 0.11.5)