FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003628017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003628017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCCACATCATCGTAAACTTCATCTCCCATGTCCAATTGTTTAGGAGG24.761904761904762No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC24.761904761904762Clontech SMART CDS Primer II A (96% over 26bp)
TTTAGTGCTGCTTCCTCCTGAAGGAACATCCTTCTGTAAGCCTTGCTTTT24.761904761904762No Hit
GGATTACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCT12.380952380952381No Hit
ATCAACGAAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG12.380952380952381Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAATGATACGGCGACCACCGAGTACCTGT12.380952380952381Illumina Single End PCR Primer 1 (95% over 21bp)
TCGGAAGAGCGTCGTGTAGGGAAAGAGTTAGATCTCGGTGGTCGCCGTAT12.380952380952381Illumina Single End PCR Primer 1 (96% over 28bp)
CTGTCCCAAGTTCTGCAGGTACCAGAGATCGGAAGAGCGTCGTGTAGGGA12.380952380952381Illumina Single End PCR Primer 1 (100% over 25bp)
TTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAGTAA12.380952380952381No Hit
ACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTT12.380952380952381No Hit
TTGATGATTCCATTCAATTCCATTCGATGATGATTCCGTACGATTCCATT12.380952380952381No Hit
GGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAG12.380952380952381No Hit
GCAACATCGAAGCCCACTACGATCTCGGCAATGATTTCTATGCCCGATGG12.380952380952381No Hit
TACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTG12.380952380952381No Hit
GCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGATACCACTGCTTCC12.380952380952381Clontech SMART CDS Primer II A (100% over 26bp)
GTGAAATGGTTGAAAAATAAGTTCAGCACTTAAAAGATTCCGTGAATTTG12.380952380952381No Hit
GACCCCATCTCTACTAAAACTACAAAATTAACCAGGTGTGGTGATGCATG12.380952380952381No Hit
ATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAAT12.380952380952381No Hit
GTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAATCTGCGTTTATA12.380952380952381No Hit
GCTCAGGAGGGCAGCGCGGGTCTGGTCCACCCGGCGAGAGAACTCAGCAA12.380952380952381No Hit
TTTAGTGCTGCTTCCTCATGAAGGAACATCATTCTGTAAGCCTTGCTTTT12.380952380952381No Hit
TGACGCAGTAAACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCT12.380952380952381No Hit
TATCAACGCAGAGTACATGGGAAGAAGTGTTATCAACTCAAAAAAAAAAA12.380952380952381No Hit
GGACAAAAGTGTCATTGAAGGATGCAAAGATATGGCAGACACCAAATACA12.380952380952381No Hit
CCTTGTGAAATGGTTGAAAAATAAGTTCAGCACTTAAAAGATTCCGTGAA12.380952380952381No Hit
CCGCACCAACAATAGCCCGATGAATATCGCCGCATTCAAAGTCTTTGGCG12.380952380952381No Hit
CTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTAGGGGTTTTAG12.380952380952381No Hit
CACCACGCTGCTGCAAGACGGCATGCTGCGCGGGCGCCACTACCAGCGCT12.380952380952381No Hit
CCTTTACACTGGGGACTTCAGAGCAAGAATGTGTGAAATAATTCACATTT12.380952380952381No Hit
AGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACGCAGGGAG12.380952380952381No Hit
CCAGTACATTTCATTCCAATCCCATCCAATCCAATCTACTCCATTCAATT12.380952380952381No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTAAGTTTATACAAAA12.380952380952381No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC12.380952380952381Clontech Universal Primer Mix Long (96% over 26bp)
AACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTA12.380952380952381No Hit
CTTCTGAATTCCCTTCTGAGTTAATGTCAAATGACAGCAAAGCACTGTGT12.380952380952381No Hit
ATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGTTGAGTCAAATTA12.380952380952381No Hit
CGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTACTCTTCTTTTATACC12.380952380952381No Hit
GTTCCGGGCTTGATCCAGGTATTGGTGGCGCAGATATCCTCGCCGCATTC12.380952380952381No Hit
CTATTGTCAGACTGCAAAACCAGAGAACCGGGCACAACCAGGCGTCCAGG12.380952380952381No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)