FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003628018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003628018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC37.142857142857142Clontech Universal Primer Mix Long (96% over 26bp)
CACGAGAACATGCCTCCCGCAAAGGATCTCCTTCATCCCTCTCCAGAAGA37.142857142857142No Hit
GAGGATGAGAAAGAAATGATGGTCAACAGATGAAGTAACCCAATATGCCT24.761904761904762No Hit
CCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTT24.761904761904762No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA12.380952380952381Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCTGGACTCGCATCCCACTATTGTCAGCCTGCATCACCAGAGACCCGGG12.380952380952381No Hit
AGAATAGACTGTAATGGAATGGACTCGAATAGAATGGACACGAATGCTGA12.380952380952381No Hit
GGGCTCTACACGATTGAACATGCTTAGGCTTCACCCCCCCACTAACGGAG12.380952380952381No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA12.380952380952381No Hit
AACGCAGAGTACATGGGAATGATACGGCGACCACCGAGATCTAACTCTTT12.380952380952381Illumina Single End PCR Primer 1 (96% over 26bp)
CCATTGAATGGACTCGAATGGAATCATTGTTGAAAGAAATGGAATGGAAT12.380952380952381No Hit
ACGCAGAGTACATGGGTGGGACCACCGAGATCTCACTCTTTCCCTACACG12.380952380952381No Hit
ACAACAAAGACGTGTTTCTGACGAGCGCCCGGCGTCGGTTTTTGAAGGAT12.380952380952381No Hit
GATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATAT12.380952380952381No Hit
GTACTCGGTGGTCGCCGTATCATTCCCATGTACTCTGCGTTGATACCTGT12.380952380952381Illumina Single End PCR Primer 1 (100% over 21bp)
GGACAGGCGGGGGACCGGCTATCCGAGGCCAACCGAGGCTCCGCGGCGCT12.380952380952381No Hit
ACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCG12.380952380952381No Hit
CAGCAATACACTGCAACTTCTGCTTCTGCGCACTTTCAAGGCTATGGGTC12.380952380952381No Hit
GGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAG12.380952380952381No Hit
GACTTAAGCCATCAAACTTCGCTTTGTTGATCACCACCAGAAGCGGGGAA12.380952380952381No Hit
GACAAGGATTTGTCCTGGGGCATTTGTGGAAAACGTAGAGGTCCTAAAAT12.380952380952381No Hit
CTTAAAAGATTCCGTGAATTTGTTGTTGTTGTTGTTGTGCAAAGAATAAA12.380952380952381No Hit
TCCCGTACTTCTGTCTTCCAGTTTTCCACTTCAAACTTCTATCTTCTCCA12.380952380952381No Hit
GGACGCCGCTGACCACCCTCAACACGGATATCAGCATCCTGTCCTTGCAG12.380952380952381No Hit
CATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGCC12.380952380952381No Hit
GACCCTGCTGGCGCTCGCGGCAGGCGCGCAGCCGCTGAATGTCGACCCGG12.380952380952381No Hit
GTACTTTTTTTTTTTTTTTTTTTTTGAGTTTATACCACTGCTTCCCATGT12.380952380952381No Hit
GAGTACTTTTTTTTTTTTTTTTTTGAGACAGAGTGTTGCTCCGCTTGTCC12.380952380952381No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT12.380952380952381Clontech Universal Primer Mix Long (96% over 26bp)
CTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATTTTACTTCAC12.380952380952381No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG12.380952380952381No Hit
TATCAACGCAGAGTACATGGGTGTCCCGAGAAAACAACATTACCAGCTGG12.380952380952381No Hit
GTACATGGGGCAGTGGTATCAACGTAAAAAAAAAAAAAAAAAAAAAAAAA12.380952380952381No Hit
GATGATAAGTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGC12.380952380952381No Hit
GTGCAAAGGATAAAAGAAATCATGAACACCGCTTATTCAGCCACACAGTG12.380952380952381No Hit
GTGTGGAGTCTGGAGACGACGTGCAGAAATGGCACCTCGAAAGGGGAAGG12.380952380952381No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)