FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003628097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003628097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGTGAGCCACCACGCCTGGCCAAATTTTTTAGTAGAGACGGGGGTCTCG35.88235294117647No Hit
TCACTGCACTCCAGCATGGGTGACAGAGTGAGACCCTGTCTCATAAAAAG35.88235294117647No Hit
CAAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACG35.88235294117647No Hit
CCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCG23.9215686274509802No Hit
ATCAACGCAGAGTACTGGGAAGCAGTGGTATCAACGCCTGTCTCTTATAC23.9215686274509802Clontech Universal Primer Mix Long (95% over 21bp)
CGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCG23.9215686274509802No Hit
TCCACAGTCTCCATGGAAATCACCTCCCTCATCTTCCTTGTGTTCATCAG23.9215686274509802No Hit
CCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAA23.9215686274509802No Hit
GTGTAAGATGTTCGCCTGCGCCAAGCTCGCCTGCACCCCCTCTCTGATCC11.9607843137254901No Hit
GACCAAGGTGATCGACGAGATCACCGCACACAAGGACGAGCTGATCGTCT11.9607843137254901No Hit
CCGGAGAGGGGCTGACCGGGTTGGTTTTGATCTGATAAATGCACGCATCC11.9607843137254901No Hit
CATCCAGGCAGCCAGGGGCTAGGACCTCATGGATCAGCAGCAAGTCCAGC11.9607843137254901No Hit
GGACTTAGCCTCTCCTCCTAGTAGTTCTGAGGAATCTGGATAGCACAGAT11.9607843137254901No Hit
CAGTCTTGCTCTGTCACCAGGCTGGAGTGCAGTGGCGCAGTCTCGGCTCA11.9607843137254901No Hit
GCTTCCCATGTACTCTGCTTGATACCACTGCTTCCCATGTACTCTGCGTT11.9607843137254901No Hit
CGCCCGCGGGTGCGCTCACTGGTGACGGCGGCGCGCTGACGCTCGGCTTG11.9607843137254901No Hit
ACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCC11.9607843137254901No Hit
GGCGACATCCGTGCCCGCGGCTTCCTGCTCGGCGGCACCGCCGGGCGCAC11.9607843137254901No Hit
CACCGGGACCCCCTGAAAGCCTAGAAATGGACGACTTGAAATATGGAGTG11.9607843137254901No Hit
CTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGA11.9607843137254901No Hit
GCCTACAGAAGCGTACATTTCAGTGGAAGAAGTCCATGATGATGGAACTC11.9607843137254901No Hit
GAAGATATTGATCCCCACCTGCAATGCAGCAGAAAGATCAATAACTTGAT11.9607843137254901No Hit
AGAAAGAGCTATCAATCTGTCAATCCTGTCCGTGTCCGGGCCGGGTGAGG11.9607843137254901No Hit
GTGTGAGCCAGCGTGAGCTCAGATGACCAGACCTTTAGTCCATGGAAAAT11.9607843137254901No Hit
GTTATTCCAGCCGCATCGTCATTCGCTCCGTTGTAGATGCTGTCGCCGGC11.9607843137254901No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGCTG11.9607843137254901Clontech Universal Primer Mix Long (96% over 26bp)
CCGTTCCATTCCATGACATTACATTAAATTACATTCCGTTCCATTGTATT11.9607843137254901No Hit
AAGCAAGGGGACCTGTAGGCTGTAGACACCTGGCCTAACCCTGCATTAGA11.9607843137254901No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA11.9607843137254901Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCT11.9607843137254901No Hit
GGGGACAGTGCCAGGTGGGGAGTTTGACTGGGGCGGTACACCTGTCAAAC11.9607843137254901No Hit
GTTAAAGGACATAATCCTGTCAGGCTTATCTTCCCGTGGTATTGTCTGGG11.9607843137254901No Hit
CCTCAAGTGATCTGTCCACTTCAGCCTCCCAAAGTGTTGGGATTACAGGC11.9607843137254901No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG11.9607843137254901No Hit
GATCCAGCGGGCGATAACGCACCTCAACGGGGTACGTGCGCCCCGAGACC11.9607843137254901No Hit
GTTTCTGGTGGCATTCAAAGAAGTGGGAAGAAAGCCCCCCACTTTTGGAG11.9607843137254901No Hit
GGCTTACATGAAACATCTTATACTTATAATCTGTCTCTTATACACATCTC11.9607843137254901No Hit
CGCTCGAACTCCCAACCTCAGGTAATCCACCTGCCTCGGCCTCCCAAAGT11.9607843137254901No Hit
CCTCTTTGTACCTCTGGTAGAATACGGCTGTGAATCCATCTGGTCCTCGA11.9607843137254901No Hit
GTGTTCAGCACATCCATCGAGGTCTTATGCATCCTTCCTGGGAAGCTCTC11.9607843137254901No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)