FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003628098

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003628098
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTCCACGCGCCCCGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGCC35.88235294117647No Hit
GGACATGGGAAGGGAGGTTCCCGAAATCCAGGCAGGAGGCCCAGACACCT35.88235294117647No Hit
GTAGAGATGGGGTTTCACAATGTTGCCCAGGCTGGCTGAGAACTCATGAG35.88235294117647No Hit
GTTGGCAATATGGCGCACTTTAAGGTATTTAAGCATGGACTCAGGAGTTT23.9215686274509802No Hit
GTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCT23.9215686274509802No Hit
GGGGTGTAGGTGTGCCTTGTGGTAAGAAGTGGGCTAGGGCATTTTTAATC23.9215686274509802No Hit
GCGTTGATACCACTGCTTCCCAGTACTCTGCGTTGATCTGTCTCTTATAC23.9215686274509802Clontech Universal Primer Mix Long (95% over 21bp)
GCCGGGATGCGGGTAGTTCTCGTTCATCAGGGTGACGTAGTAGTACACGT11.9607843137254901No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT11.9607843137254901No Hit
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAAAAGGGG11.9607843137254901No Hit
CAGCGGTGATCGCCTCGTCGGTGTAGTGCACCTTGTGGTGATCCTCGTAC11.9607843137254901No Hit
CTTCCCGTGGTATTGACTGGCCTGGCGGGTGAAAAATTAGTGGTGAGGGG11.9607843137254901No Hit
CGTCAGCAATTCAGAACGCCACATAGCGTAATTTTTTTACTGGGCAACAC11.9607843137254901No Hit
ATTTTGAAGAGCCTCCTTGGGGAGATTTCGATGGGCATCTTTGGGGCCAC11.9607843137254901No Hit
GTAGTAGATGATGTTGGCCAGATTGTATTCTTGGTAATTTTGCAGCACGT11.9607843137254901No Hit
CGCTATGAACGCTTGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTT11.9607843137254901No Hit
CCCTTGGGCTCTATGGGAAGGACCAGCAGGAGGCAGCCCTGGTGGACATG11.9607843137254901No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAAGCAGAGTACATGGGAA11.9607843137254901No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.9607843137254901No Hit
CTTCACATTTGAAGGGTTTCTCTCCAGTATGAATTCTCTTGTGTCTAGTA11.9607843137254901No Hit
GTGCAGCCTCGACCACCTGTGCTCAAGCAATCCTCCCATCTCCATCTCCC11.9607843137254901No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT11.9607843137254901Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATACACATCT11.9607843137254901No Hit
ATTCAGACCTTACTCCCTCAAAATGGCTTCCAAAAGTGATGCTGAGGAAA11.9607843137254901No Hit
TTGGGAGGCTGAGGCGGGCGGATCACAAGGTCAAAAGATTGAGACCTTTC11.9607843137254901No Hit
NTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTG11.9607843137254901Clontech Universal Primer Mix Long (96% over 26bp)
GTCTTAGGGTAACTGCCCTGTCTTAGGGTAACTCAATTCTTCATCACAGC11.9607843137254901No Hit
CGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCC11.9607843137254901No Hit
ATGTTTTTCTTACAGCAAGCACATCTAATAATAATGCTCAAAAAATGTGA11.9607843137254901No Hit
TCCCCCCCGCGAAGGGGGTCAGCGCCCGTCGGCATGTATTAGCTCTAGAA11.9607843137254901No Hit
ATTATAAGTATAAGATGTTTCATGTAAGCCCTGTCTCTTATACACATCTG11.9607843137254901No Hit
CCTCTTTCCTATCCTTTTTGCTCTCTTCTTTCTCCTGCCCTTCTTTCTTC11.9607843137254901No Hit
GTATTGCGCGGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG11.9607843137254901No Hit
CACCAACCTGATGCCATTAGGATGCCAGTAGGGTGTCTGACAACCCCTGT11.9607843137254901No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG11.9607843137254901No Hit
GATTTCCCACGATCGCGACTTGTGCGCCCATGACACCATCATTATTGACG11.9607843137254901No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11.9607843137254901No Hit
ATCCGAATGTAATGGAATGGAATGGAACGGAATGGAATTGAATGGAATGG11.9607843137254901No Hit
GTATCAATGTCTCTGCTGATTGCACTGGTCTGAAACTCCCTTTGGATTAG11.9607843137254901No Hit
GGATAAAAGAGGGAAGAATCAAATAGATGCAATAAAAATGATAAAGGGGA11.9607843137254901No Hit
AGGGCAGCGTGATCATGTCCTTCGCTGCCGATGCCGCTGCCGCGGCGCCC11.9607843137254901No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)