FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003628115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003628115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACTATACACTTTCCACTGCAGGCGGGTTATATATTGACAGCAAATTTC34.615384615384616No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC34.615384615384616Clontech Universal Primer Mix Long (96% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT23.076923076923077Clontech Universal Primer Mix Long (96% over 26bp)
ATAACAAACTCACTCTTTTTTTTTTTTTTTTTGAGACGGAGTTTTGCTCT23.076923076923077No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA23.076923076923077Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTTTAGAGGGTGGTAGGTTGGCAATGCCACTTTAAGGGATAGCTTAACC23.076923076923077No Hit
TTCATGGACGTTCTGTTGATGAATTAATCTCCGCATTGCAGCAAGGTGCA11.5384615384615385No Hit
CACCAGGAGTGGAGCCTGCGGCTTAATTTGACCCAACACGGGAAACCTCA11.5384615384615385No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11.5384615384615385No Hit
GCCACTACGGAACCCTTCAACCTCCTTGTAATTGCTGCCAACGCGCAGAA11.5384615384615385No Hit
GTGAAGTGGTTTTGCTGTAGAACTGAATGCCACACTTAGAATTGTGGGGG11.5384615384615385No Hit
ACTAAACACCATTCAGAAGATGCTAAGACCCATGAATTGCAACAGGAAAG11.5384615384615385No Hit
GTCCCCGAAGCCACGGGCACACTGCAGCCGGTGTAGCACCAGGCCTGAGC11.5384615384615385No Hit
ATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCT11.5384615384615385No Hit
GCACCAGGCAGACGCGTGCCATGCCTACCAGATCATTCACCGCAATGGGA11.5384615384615385No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA11.5384615384615385Clontech SMART CDS Primer II A (96% over 26bp)
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT11.5384615384615385No Hit
CCAGGTCGACACGACCAACATCCTGTTCATCTGCGGCGGCGCCTTCTCCT11.5384615384615385No Hit
TGTCCGGCACTGCCCGGAGTAAGACATTGACGGGAGCTGGGGGCTTCGTG11.5384615384615385No Hit
GGATAAGCAAGTTGATGACCAACCTGTGCGCGTAAAGCGGAGGCAAAAAA11.5384615384615385No Hit
GTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGC11.5384615384615385No Hit
GTTTTAACTCCAGGGGCGAGTGGCAAGTCATCAGTCATAGCGAAGAGAAA11.5384615384615385No Hit
ACGCAGAGTACATGGGAGGAAATAATTCTGTAGTGATAGGTTTTAATGTG11.5384615384615385No Hit
GATACAGAGTCTTGCTCTGTTGCCAGGCTGGAGTGTAGTGGCGCGATCTC11.5384615384615385No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG11.5384615384615385Clontech Universal Primer Mix Long (96% over 26bp)
GTTCTATTCCTAAAGCTTCAGCTAGTATTATTGCCAGAGTATAGGCTTCT11.5384615384615385No Hit
TCCCATGTACTCTGCGTTGCTCCCACTGCTTCCCATGTACTCTGCGTTGC11.5384615384615385No Hit
GAACGTTGTTAAGTGGGACGAAATTAACAAGCGGCTAGAAATGGCAACTC11.5384615384615385No Hit
CTTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAG11.5384615384615385No Hit
CCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGA11.5384615384615385No Hit
CGACAATTCTTGGCGATCGCGTGCGACTTGAATTAAATACCAGCTTCACG11.5384615384615385No Hit
GGAAAAAATTATGAGAACACAAATAATCGAAGTCGAACAAGTAGAGATGT11.5384615384615385No Hit
CCGCAACATTCCGCCCACCCCTCGCCGCGGCCGGCGCGCTATATCGGGGA11.5384615384615385No Hit
GTCGTCGACGGCCGTCATCGCGATCATCGTGAGGTCGACCGGCTCGCGCT11.5384615384615385No Hit
CACCCCCGACGCGCTCCGCGCGGAACGGGAGGCGAAGCTCGCCACCAAGC11.5384615384615385No Hit
ATAGAATTAGGACTGAGGCTTAAAGCTTGTAGGTCGGGTAAAATTTGTCC11.5384615384615385No Hit
CACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAATCCTGTC11.5384615384615385No Hit
AGACATAGCCATGACATTTACTCCAACTTTGATTCATGTAGTGCCATTTG11.5384615384615385No Hit
TCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAA11.5384615384615385No Hit
GCTTTGGAAGAAATTAAGGGAGACAAGAAAGTTGAGGCGGTTAAGACTTC11.5384615384615385No Hit
GCCTGCCTCTCTGCCCTTGTCCTCATCACCGACCGGCATGGGCTGCAGGA11.5384615384615385No Hit
CTATAAAAGCTCTTACACGTCAAAATAGATCTTACTTTTTGAATGCTTGA11.5384615384615385No Hit
CGTCCACGAGCACGGCCTCTGGCGTGCCCGCGCGGGCGAGCGCGTCCTCG11.5384615384615385No Hit
CCCCTGGGGCAGGGCTCCCATGGGCAGCGCCTGCATCAGCAGCGGGGTGG11.5384615384615385No Hit
GCCCGAAGCGCTGCGCGCCCGCCGGGCGGAGATCGCTTCCGCCGCGGCGG11.5384615384615385No Hit
CAAAGTATGGCAACCAATTCAAGTAAAATGATATCCAGAAACTTAGGAAG11.5384615384615385No Hit
AGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAA11.5384615384615385No Hit
CTCGCCCCTCTCCTTTAGATCTGGGCGTGCGGGTTTCCCTGCACCCAGCT11.5384615384615385No Hit
GCCAAGAAGGCAGTGTTGAAAGGTGTCCACAGCCACAAAAAGAAGAAGAT11.5384615384615385No Hit
TTGGATAGGCTTGCCATCAACTTCAATCGTGTCTCTTTCTGGATCGACTT11.5384615384615385No Hit
TGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAA11.5384615384615385No Hit
GGGATGAGAACAAGAATCCGGCATACAAGCTGTTTTTGGGATCTGGCGAT11.5384615384615385No Hit
TTTGAGGATATAGCAAAGAGGTAGCCATCTGCAATCCAAGGACAGAGGAT11.5384615384615385No Hit
GATCAGGGCCGCCATCAACAGGATCGCCAGTCTTTTTTTCATCTTGCTCC11.5384615384615385No Hit
TGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCA11.5384615384615385No Hit
GCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTT11.5384615384615385No Hit
GTAAAACCCCGTCTGTACTAAAAGTACAATAATTAGCTGGGCATGGTGGC11.5384615384615385No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)