FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003628127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003628127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG39.090909090909092Clontech Universal Primer Mix Long (96% over 26bp)
GTCCGTATGCGAGAAGGGTGAAGTCGGGCGGCGCCTCGCCGGCAACCTGC39.090909090909092No Hit
GTCCAGGGCCGCGCGCTGGCGGGCCTTCTCGTCGTCGGTGGGATTGCCGC26.0606060606060606No Hit
GACTGGGTGTAGCGCAGCTCCCAGTTGCGGTCGTCGGTGGTGATCACCGT26.0606060606060606No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC26.0606060606060606Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG26.0606060606060606Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA13.0303030303030303Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGATGAT13.0303030303030303Clontech Universal Primer Mix Long (96% over 26bp)
GCTGACCCAAGCAGAGATGAATGGGGCCGCATCGCGGCAAGTCACATTAG13.0303030303030303No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT13.0303030303030303Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG13.0303030303030303Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT13.0303030303030303Clontech Universal Primer Mix Long (96% over 25bp)
GGATCACAGCCGCTCCATCTTCGGCAGCCAGACGCGCAGCAGCGAGAACA13.0303030303030303No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCCGATGAACATCGCGTATG13.0303030303030303No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCCGATGAACATCTCGTATG13.0303030303030303No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC13.0303030303030303Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC13.0303030303030303Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA13.0303030303030303Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCTGGACTCTGCGTTGATACCACTGCTTCCC13.0303030303030303Clontech Universal Primer Mix Long (96% over 26bp)
GTGTCTGGGTTTCATCCATCCGACATTGAAGTTGACTTACTGAAGAATGG13.0303030303030303No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG13.0303030303030303Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTT13.0303030303030303Clontech Universal Primer Mix Long (96% over 26bp)
ATATCATATAAGTAATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGA13.0303030303030303No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT13.0303030303030303No Hit
CGATTGCGGCACGGCGGCCGCGGACTGATTCCATGGCAGCCTCGTAAGCG13.0303030303030303No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)