FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003628128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003628128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGTGCCCGTCAACTGGCCGACGCCAAGGCCGTCGTGAAGAACCTGACC39.090909090909092No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG39.090909090909092Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA26.0606060606060606Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26.0606060606060606No Hit
TCGCCACCGTGCTGCTGGCCGGCTGCTCGAGCTTTACCCGACCCAGTGGC26.0606060606060606No Hit
GACGATGGCATCACGCTGGTCAACATCGGCGTCGGCCCCTCCAACGCCAA26.0606060606060606No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCCATGTACTCTGCGTTGATA26.0606060606060606Clontech Universal Primer Mix Long (96% over 27bp)
GCTGAACTGCTCGACGTTCGCGAGAGACATGAGCTGGACAAGAAGAAGGC13.0303030303030303No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA13.0303030303030303No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT13.0303030303030303Clontech Universal Primer Mix Long (96% over 26bp)
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAGTAAACTGCGT13.0303030303030303No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC13.0303030303030303Clontech SMART CDS Primer II A (96% over 26bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAATGCTTTGTAACAAA13.0303030303030303No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA13.0303030303030303Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG13.0303030303030303Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT13.0303030303030303Clontech Universal Primer Mix Long (96% over 26bp)
GGGAGTCCGAACTAGTCTCAGGCTTCAACATCGAATACGCCGCAGGCCCC13.0303030303030303No Hit
GGCAAAGGGGAGCGCTGGTTCTGCAGCGAGAATGAGGCCCGCGCGGCAGG13.0303030303030303No Hit
GGTCAGCGAGATCGACCTGGTGCTGATGGACGTGATGATGCCAGAGATGG13.0303030303030303No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAGCAGTGGTAT13.0303030303030303Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG13.0303030303030303Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG13.0303030303030303Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC13.0303030303030303Clontech Universal Primer Mix Long (96% over 26bp)
TAGAAAGACCAGTCCTTGCTGAAAGACAAGTCTGAATGCTCCACTTTTTC13.0303030303030303No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)