FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003628153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003628153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTTACATGTAAACCAACAAATCACCTTGCCCATTTCATGGAT37.317073170731707No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA24.878048780487805Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCCAGGTGCCGCCCGGCAGCGTGGACGCGCACTGCCACGTGTTCGGCC24.878048780487805No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT24.878048780487805Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGCATCAACGCAGAGTACATGGGAAGCAG24.878048780487805Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24.878048780487805No Hit
GACCATGCTGTCCCGGGTGGTACTTTCCGCCGCCGCCACAGCGGCCCCCT12.4390243902439024No Hit
GTGCTGGCCATGAAGAATGGGGACGTGGTGGACAAGTTTGTGGGCATCAA12.4390243902439024No Hit
TTTTAGTAGAAACGGGGTTTCACCATATTGCCTAGGCTGGTCTTGAACCT12.4390243902439024No Hit
AATGTAGAGAGAGGTAGAGAGAGAAATATGAACTCTAACAAAGGACTGAG12.4390243902439024No Hit
GGTAGCGGTCTTCAAAGCCGGCCAAACCCACGCGCGCAAGCCACGACATC12.4390243902439024No Hit
TTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTA12.4390243902439024No Hit
GCTTTGTGCTGTCTGTCGTCACTATCATCATCTGCTTCACCTGCTCCTGC12.4390243902439024No Hit
GTGGCGGGCCAGCTCTTCGACGACGCGGACCAGCCGAGAGTCCTGCACCT12.4390243902439024No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG12.4390243902439024Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTA12.4390243902439024No Hit
TGCTGGGAGCTGTCCAAGTCAAGAGGAGGACAAAGAAAAAGATTCCTTTC12.4390243902439024No Hit
TCTTTGTGCTGTCTGTCGTCACTATCATCATCTGCTTCACCTGCTCCTGC12.4390243902439024No Hit
GTCGCAATGGCGGGGGACGGCGTCAACGATGCTCCAGCGCTTGCCCGGGC12.4390243902439024No Hit
GCTGTGCATCTCAGTCGCACACAAGGCAGCTGTCTCAGGCTTTACAAGCT12.4390243902439024No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT12.4390243902439024Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAAT12.4390243902439024No Hit
CTCTGACTCTGTGTCATAGCTCTCCTCATCCTCACTCTCTGGCAGCATCT12.4390243902439024No Hit
GAAAAAAGAATATGTTTGAACACATTGAAAGGAGTTTTATGCTCTATCAG12.4390243902439024No Hit
GGCATAATTATAACAAGCTCCATCTGCCTACTACAAACAGACCTAAAATC12.4390243902439024No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG12.4390243902439024Clontech Universal Primer Mix Long (96% over 26bp)
GGCAGGAGGATTGCTTGAGCCCGGGAGTTCAAGACCAGCCTGGGCCTGTC12.4390243902439024No Hit
CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA12.4390243902439024Clontech Universal Primer Mix Long (95% over 22bp)
ACACCAACCTCCTCATAATCCTTCTCAAGGGCAGCCATGTCCTCACGGGC12.4390243902439024No Hit
CGGCGGGACCTCTGAGAATTGGGATACAGGACCCAAAAGGCTGAAAGGGG12.4390243902439024No Hit
GGTTAATATGAAGCTTGGAATATTTTTCAGTGTTTTAGTAAAACTGCAAG12.4390243902439024No Hit
CTCGAGGCCTCTGAAATCCCCTTTGGGCCCTTGGAATATTCCCAGACCTC12.4390243902439024No Hit
GTAAAGCGCTGGGCGGGGCTTCTGGTGGTTATACCGCGGCGCGCAAAGAA12.4390243902439024No Hit
GGCATAATTATAACAAGCTCCATCTGCCTACGACAAACAGACCTAAAATC12.4390243902439024No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)