FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003628154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003628154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTGAATAAACCAAACATATCTAAAGGTAATTTCCTCTGGAGTCTTTT37.317073170731707No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT37.317073170731707Clontech Universal Primer Mix Long (96% over 26bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA24.878048780487805Clontech Universal Primer Mix Long (96% over 26bp)
TATACAGGCAGGACACACACAGCACACATGGGGCGTAAGGAACCGTGCCT24.878048780487805No Hit
CACCGGCGGCGTGCAGGGCCTGCAATTCTTCATCGGTCACCGAGCGCTTG24.878048780487805No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA12.4390243902439024Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAAAAAACAAAAACCACTCTAAGTGTCCAAATATTGGAAAAAAAGAAGCA12.4390243902439024No Hit
GTACATGGGCAAGTCAGCTGAAGTCATTGGCAACAGCAATTGAAGTGAAG12.4390243902439024No Hit
GGCAGTGAAGGTCTCTGCGCTAATCACATATATTTCTTTGGATAAAGCGT12.4390243902439024No Hit
GTCACGCAGCTGCCTTCGACGGCCTGCCATTGACCGTGCACGTGCCCGGC12.4390243902439024No Hit
GGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTATT12.4390243902439024No Hit
TGTCAATGCTGCCATTGCCACCATCAAGACCAAGCGTACCATCCAGTTTG12.4390243902439024No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTATACCAAAGCTT12.4390243902439024No Hit
TTACAAAACAGCAGCTGGAAAGAGAAATGTAGGTGGCAGACGAGCCAGGC12.4390243902439024No Hit
CAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTA12.4390243902439024No Hit
GCCCAGGCTGGTCTTGAACTCCCGGGCTCAAGCAATCCTCCTGCCCTGTC12.4390243902439024No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT12.4390243902439024Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC12.4390243902439024Clontech Universal Primer Mix Long (96% over 26bp)
ACCATGTAGGACACATTTTGGTCTTTCTTTTGGTAGTCACCTTCATGTTT12.4390243902439024No Hit
GTGACATGTAGGTAGGAAGCACTGAAAATAGTGTTCCCAGAGCACTTTGC12.4390243902439024No Hit
AGTGAGGGGCAAGCAGATCAAGCTCGACCAGACCTTCACCAATACCTTCG12.4390243902439024No Hit
GGGGTAAGGCGAGGTTAGCGAGGCTTGCAAGAAGTCATCAAAAAGCTATT12.4390243902439024No Hit
GGTTGCTGCTGTGATATGGAGGAAGAAGAGCTCAGGTGGAAAAGGAGGGA12.4390243902439024No Hit
GCGCGCACCAGGCCGGTCAGGTCGCCCCTCACCAGGGTGACCCCAGCGCT12.4390243902439024No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG12.4390243902439024Clontech Universal Primer Mix Long (96% over 26bp)
TCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCT12.4390243902439024No Hit
GCCAGTAAGAGGGAAGGCAGGATTTGCATCAGGTCTGTTAGAGTCTGGAA12.4390243902439024No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT12.4390243902439024Clontech Universal Primer Mix Long (96% over 26bp)
CAGTATGTGCTGAGGGTTCTAGTTCAACAAAAGCCAAGGCTAAAAAAGGT12.4390243902439024No Hit
CGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGC12.4390243902439024No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC12.4390243902439024Clontech Universal Primer Mix Long (96% over 25bp)
GGTATGCGCCGCCGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAA12.4390243902439024No Hit
GCTGGCACCCGGTAGACTGTCTGGAAGAGCCCTGGGGTGCCTTTGGGATG12.4390243902439024No Hit
GAAGAAAAGGCACTGACCATGGAAGTGATCCGCCAGGTGAAGGGAGACCT12.4390243902439024No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)