FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003628156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003628156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA36.521739130434782Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG36.521739130434782Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG36.521739130434782Clontech Universal Primer Mix Long (96% over 26bp)
GCCGTGGCCGGGTCGGCCACGTCCGCCTGCGGCGCCTTGGCCAGCACCCG24.3478260869565215No Hit
CTCCCAGCGCTTCGAGCGTGTCGATGTTGCGCTCGAAGATCGCCTCTGCC24.3478260869565215No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT24.3478260869565215Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC24.3478260869565215Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC24.3478260869565215Clontech Universal Primer Mix Long (96% over 26bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC12.1739130434782608Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT12.1739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
CAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA12.1739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC12.1739130434782608Clontech SMART CDS Primer II A (96% over 26bp)
GGAGGGAGTCAAAACACGGACGCGCGCGCATGGTATTGCGCGGCTCGAAT12.1739130434782608No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG12.1739130434782608Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG12.1739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCACTAAAACAAAT12.1739130434782608No Hit
CGACCATCCTGTTCGCCTCGTCGAGCCGCCCCTGGGCCTTGAGTTCCTGG12.1739130434782608No Hit
GAACTGCGCAACGAACTGGTTGGCCGGATGGTCGTAGAGCGCCAGGGGCG12.1739130434782608No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC12.1739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTACCCATGTACG12.1739130434782608Clontech SMART CDS Primer II A (96% over 25bp)
TCCTCCAGAGGAGCGGGGCTCTTCGTGATTCCATAGATGTCTGGCGAGCT12.1739130434782608No Hit
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC12.1739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC12.1739130434782608Clontech SMART CDS Primer II A (96% over 26bp)
ACTTTGAAAAAATTAGAGTGTTAAAATCAGACACCAGCCTACAAGAAACC12.1739130434782608No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA12.1739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC12.1739130434782608No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12.1739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
ATACATGGGAAGCAGTGGTATCAACGCAGAGTAAATGGGAAGCAGTGGTA12.1739130434782608Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG12.1739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT12.1739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC12.1739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
TGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG12.1739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
GTGTAGGGCTTGCTGCTTTGGTTGCAAAAAATATCCAGGTTGTAGCCGCC12.1739130434782608No Hit
CCGCAAAAAAAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCT12.1739130434782608Clontech SMART CDS Primer II A (100% over 25bp)
GGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGG12.1739130434782608No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)