FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003628239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003628239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22
Sequences flagged as poor quality0
Sequence length100
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAAGTACTCTGCGGAGACCGGACCCGGCGAGAGTCACCAGTGTGACCG313.636363636363635No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA313.636363636363635Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTCTCGTCACTCACCTAGCAGGCCACCTGTTCGTCGACGTCATGGA14.545454545454546No Hit
GCCCAGGAGGGTGCCGTCGACGTATGAGATCAGTCGTTTGCGCGATCACC14.545454545454546No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG14.545454545454546Clontech Universal Primer Mix Long (96% over 26bp)
CCCCATGATGTCTTTACCCGTCACGGTGACGACCTCGAGATGGTTGTCAC14.545454545454546No Hit
CCGCATCGGCCGGACTGGCCGTAACGGTTCGACCGGCGAGGCGATCTCGT14.545454545454546No Hit
CGGTCGAGATCAGTTGGGGAATTTTCAATACAGCATTCCTGAGGGTGAGC14.545454545454546No Hit
GACGCGCGGCTGGGATCTTGCAGGACCCGCGCACGCACTCTAACTGGAAA14.545454545454546No Hit
TCATTTGCTTCTCTGCTCTTTTTTCACAAGCATTGCTCCTGCCTTAGGAG14.545454545454546No Hit
ACCGTGTGCGTCGCGCCGAAGCGGCGTGCAACCTCCAGCCTTCCCTCGTC14.545454545454546No Hit
CCCTGGACTGGAGCCCAGCAAGCCTGGATGCAGAACACCACTTTCTGACC14.545454545454546No Hit
CATAGACACGCACTGGCAGGCCTTCCTGCCGCGCCTTGAGCTGCAGCGCC14.545454545454546No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA14.545454545454546Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCATTCGAGTTGCGAAGGAAAGCTGGGGATTGCTCTCGAGTGACTGC14.545454545454546No Hit
ATATTGCACAATGGGCGGAAGCCTGATGCAGCAACGCCGCGTGCGGGATG14.545454545454546No Hit
AGCGTGACGAGCTTGAATTCGAGCAGCGTCACGAAAATCTGGATCGCGAT14.545454545454546No Hit
TGCGGGACCGCACCGTCATGCCCGGGGCCGAGCCTGCTCCGCAGACGGGA14.545454545454546No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)