FastQCFastQC Report
Wed 27 Apr 2022
EGAF00003628240

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003628240
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22
Sequences flagged as poor quality0
Sequence length100
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGGTGTCCGCGCTAGCCGTCACGCACTCGTCCAACAATCCTTCGTCCA313.636363636363635No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT313.636363636363635Clontech Universal Primer Mix Long (96% over 26bp)
ATCGAGGAGGTAGAAGGAGTTCCCTCCTGAAAGATAGATTATCTCGGCTT14.545454545454546No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGATAC14.545454545454546Clontech Universal Primer Mix Long (96% over 26bp)
GCCTGGCCAAGATCATCTATCTGAGCTTTTCGGGCCTCGGGCTGAAGACG14.545454545454546No Hit
CCACCCGGCTGCTCGGCACGGCCCGGGGCGCTGTTGCCCCAGCGGATCGG14.545454545454546No Hit
CCGTTTACAGCGTGGACTACCAGGGTATCTAAGCCTGTTCGCTCCCCACG14.545454545454546No Hit
GGCTATGGCTCCATGAACGGCTGGGTGGCCGACCTGATCAGCCAGGAGGT14.545454545454546No Hit
TCGTTAATGAGCTGCCACACGAATGTGAGGTCGGATAGGTCTGAGAAATC14.545454545454546No Hit
GTGTCGTCACCCGCGATGGTGCCAAGCACTGTATGGACACCCGAGGTGTC14.545454545454546No Hit
CTCTCCAAACGACTCAGGAGGCTTGACTCCCTTGAGTCCACCCAGTGAGC14.545454545454546No Hit
GCACGCCGACACCAGCCTCTACCCCGTGCCGCCGGGCGTGGACGAGGAAG14.545454545454546No Hit
TCCGGGCACAGCTGCGACGACCTCGCCAAGAAAGCAGGGGTCACTCATCC14.545454545454546No Hit
GTCTGCGATATGTGGCTGCCCCTATCGCGGTCACGGGGATTACGGCGGGA14.545454545454546No Hit
GACGTCGGAGCGCGGTTATTGACACGGCCCGGTAAAATGCCCTGTCTGCC14.545454545454546No Hit
GGCACAGACTGGTCTTCAGTGTCCAGGGTCCTTCCCTTCCTTGGTTTCTT14.545454545454546No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCTTGTACTCTGCGT14.545454545454546Clontech Universal Primer Mix Long (96% over 26bp)
TAATGACAGTTTGCAGACTTTTCCTTTCCAGTTAGAGTGCGTGCGCGGGT14.545454545454546No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)