FastQCFastQC Report
Tue 10 May 2022
EGAF00003628253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003628253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1057334
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG105090.9939148840385347No Hit
GTATATAGGCTGAGCAAGGGGTGGTGAGGTTGATCGGGGTTTATCGATTA66210.626197587517284No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG66090.6250626575897493No Hit
CTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTG60270.5700185561043152No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC53390.504949240258991No Hit
GTGTGTACGCGCTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTT46500.4397853469197056No Hit
CTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAAT43790.4141548460562131No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG42550.4024272368050209No Hit
CCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATAC42260.39968448948014534No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC41680.3941989948303942No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC37590.3555167998002523No Hit
CCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCG36740.347477712813548No Hit
TTCCAGTACACTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTA35900.33953320332080494No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG32350.3059581929645694No Hit
CAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCT30650.2898800189911608No Hit
CCAGTACACTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTAGG28600.27049163272910925No Hit
TACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCA28520.2697350127774194No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG25160.23795697480644717No Hit
GTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACC24050.22745887297675096No Hit
GTACATGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGAT21560.20390907698040542No Hit
GGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT21310.20154463963137476No Hit
CTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAAGTTTCATA21060.1991802022823441No Hit
CACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCC20940.19804527235480934No Hit
GTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT20590.19473506006616642No Hit
GTTTTAGATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAA20510.1939784401144766No Hit
GTGTACGCGCTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTA19930.18849294546472545No Hit
ACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAA19910.18830379047680298No Hit
GCATAGTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA19810.1873580155371907No Hit
GGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAG19220.1817779433934783No Hit
CCTTTGAAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAG19110.18073759095990483No Hit
GTCTTTACGTGGGTACTTGCGCTTACTTTGTAGCCTTCATCAGGGTTTGC18970.17941350604444764No Hit
CTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCAATGC18910.17884604108068028No Hit
CTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCATTCCAGTGAG18630.1761978712497659No Hit
GGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAA18340.17345512392489035No Hit
ACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAAGTTTCATAAGGG18200.17213103900943316No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC18180.1719418840215107No Hit
GCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAACACTAC18000.17023948913020862No Hit
TGTTACGACTTGTCTCCTCTATATAAATGCGTAGGGGTTTTAGTTAAATG17890.16919913669663514No Hit
GCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAAC17650.16692927684156567No Hit
ATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAGCGCA16980.16059258474616347No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC16940.16021427477031855No Hit
CTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCACCTTCGA16880.1596468098065512No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA16660.1575661049394042No Hit
TCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAATCC16620.1571877949635593No Hit
TAGCTATTGTGTGTTCAGATATGTTAAAGCCACTTTCGTAGTCTATTTTG16310.15425589265076126No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT16170.1529318077353041No Hit
CTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTAGGGGTTTTAG15830.14971617294062237No Hit
CAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACA15710.14858124301308764No Hit
GATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCA15500.14659511563990185No Hit
CTACGAGCCACAGCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCC15240.14413610079690997No Hit
GGCTACACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTTACTTTGT15160.14337948084522015No Hit
CCCCAATAAAGCTAAAACTCACCTGAGTTGTAAAAAACTCCAGTTGACAC15090.14271743838749157No Hit
CGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCACC15000.14186624094184053No Hit
GTTCTGTAATCGATAAACCCCGATCAACCTCACCACCCCTTGCTCAGCCT14930.14120419848411192No Hit
TATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACT14850.14044757853242212No Hit
GCGGTATATAGGCTGAGCAAGGGGTGGTGAGGTTGATCGGGGTTTATCGA14570.13779940870150775No Hit
GAAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCC14570.13779940870150775No Hit
CTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAAC14450.13666447877397303No Hit
GCTTCATATCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATC14450.13666447877397303No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG14390.13609701381020567No Hit
TAGTAAGATTACACATGCAAGCATCCCCATTCCAGTGAGTTCACCCTCTA14380.13600243631624445No Hit
GGCTTTAACATATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAG13770.1302332091846096No Hit
AGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCACCCCCTCCCCAAT13660.1291928567510361No Hit
TAACATATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACC13340.12616637694427682No Hit
CCTCATGGGCTACACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTT13090.12380193959524614No Hit
ATCCACCTTCGACCCTTAAGTTTCATAAGGGCTATCGTAGTTTTCTGGGG12930.12228869969186654No Hit
ATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGA12930.12228869969186654No Hit
GCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCCTCTAGAGGAGCC12910.12209954470394407No Hit
GGGCTACACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTTACTTTG12820.12124834725829303No Hit
GTTACGACTTGTCTCCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGT12820.12124834725829303No Hit
GCCTAGCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAA12690.12001883983679708No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA12650.11964052986095217No Hit
CTCATGGGCTACACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTTA12580.11897848740322359No Hit
ATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATATGTTAAAG12550.11869475492133989No Hit
TTACAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTG12110.11453334518704592No Hit
GATTACACATGCAAGCATCCCCATTCCAGTGAGTTCACCCTCTAAATCAC11950.11302010528366627No Hit
TCATATCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAAC11910.11264179530782138No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT11880.11235806282593769No Hit
ACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTT11880.11235806282593769No Hit
ACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACACAATAG11640.11008820297086824No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC11520.10895327304333352No Hit
GCTAAATCCACCTTCGACCCTTAAGTTTCATAAGGGCTATCGTAGTTTTC11350.10734545564599265No Hit
TCATCAGGGTTTGCTGAAGATGGCGGTATATAGGCTGAGCAAGGGGTGGT11320.10706172316410899No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT11250.10639968070638038No Hit
GAGCAAGGGGTGGTGAGGTTGATCGGGGTTTATCGATTACAGAACAGGCT11070.1046972858150783No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA10820.10233284846604762No Hit
AGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAAC10820.10233284846604762No Hit
ATTCCAGTGAGTTCACCCTCTAAATCACCACGATCAAAAGGGACAAGCAT10710.10129249603247413No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)