FastQCFastQC Report
Wed 27 Apr 2022
EGAF00003628254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003628254
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1057334
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG95860.9066198571123221No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG68980.6523955533445439No Hit
GTATATAGGCTGAGCAAGGGGTGGTGAGGTTGATCGGGGTTTATCGATTA60540.5725721484412684No Hit
CTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTG56200.5315255160620957No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC54610.5164876945222607No Hit
GTGTGTACGCGCTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTT41800.3953339247579289No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC41260.39022674008402264No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG39950.3778370883751019No Hit
CTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAAT39340.372067861243467No Hit
CCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATAC38410.363272154305073No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC36510.3453024304524398No Hit
CCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCG35300.33385855368313133No Hit
TTCCAGTACACTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTA34570.32695439662396175No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG32020.3028371356638489No Hit
CAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCT30820.2914878363885016No Hit
TACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCA28200.2667085329706602No Hit
CCAGTACACTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTAGG26650.25204902140667No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG26630.2518598664187475No Hit
GTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACC22970.21724450362893846No Hit
GTTTTAGATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAA22190.20986745909996274No Hit
GGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT21720.2054223168837851No Hit
CACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCC20800.19672118743935219No Hit
GTACATGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGAT20160.19066822782583365No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC19400.18348033828478041No Hit
ACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAA19390.18338576079081917No Hit
GTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT18990.1796026610323701No Hit
CTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAAGTTTCATA18940.17912977356256396No Hit
GTGTACGCGCTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTA18910.17884604108068028No Hit
GCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAACACTAC18650.17638702623768837No Hit
GCATAGTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCA18640.17629244874372715No Hit
GGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAA18640.17629244874372715No Hit
CTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCATTCCAGTGAG18380.17383343390073525No Hit
GCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAAC18130.17146899655170458No Hit
ACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAAGTTTCATAAGGG17940.16967202416644125No Hit
CTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCAATGC17900.16929371419059636No Hit
CCTTTGAAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAG17810.1684425167449453No Hit
GGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAG17710.16749674180533303No Hit
CCCCAATAAAGCTAAAACTCACCTGAGTTGTAAAAAACTCCAGTTGACAC17210.1627678671072717No Hit
GTCTTTACGTGGGTACTTGCGCTTACTTTGTAGCCTTCATCAGGGTTTGC17050.16125462720389205No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC16440.1554854000722572No Hit
ATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAGCGCA15880.15018906041042848No Hit
TGTTACGACTTGTCTCCTCTATATAAATGCGTAGGGGTTTTAGTTAAATG15870.15009448291646726No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA15690.14839208802516518No Hit
CAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACA15690.14839208802516518No Hit
GATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCA15520.14678427062782431No Hit
CGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCACC15510.1466896931338631No Hit
TCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAATCC15360.1452710307244447No Hit
TAGCTATTGTGTGTTCAGATATGTTAAAGCCACTTTCGTAGTCTATTTTG15230.14404152330294875No Hit
GGCTACACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTTACTTTGT15210.1438523683150263No Hit
GTTCTGTAATCGATAAACCCCGATCAACCTCACCACCCCTTGCTCAGCCT15180.1435686358331426No Hit
CTTACCATGTTACGACTTGTCTCCTCTATATAAATGCGTAGGGGTTTTAG15150.1432849033512589No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG15140.1431903258572977No Hit
AGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCACCCCCTCCCCAAT15130.14309574836333647No Hit
TATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACT15090.14271743838749157No Hit
CTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCACCTTCGA14990.14177166344787928No Hit
GAAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCC14730.13931264860488737No Hit
GGCTTTAACATATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAG14490.13704278874981793No Hit
GCTTCATATCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATC14480.1369482112558567No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT14040.13278680152156272No Hit
CTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAAC13870.13117898412422188No Hit
CTACGAGCCACAGCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCC13740.1299494767027259No Hit
TAACATATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACC13560.12824708181142383No Hit
GCCTAGCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAA13560.12824708181142383No Hit
TAGTAAGATTACACATGCAAGCATCCCCATTCCAGTGAGTTCACCCTCTA13400.1267338419080442No Hit
GCGGTATATAGGCTGAGCAAGGGGTGGTGAGGTTGATCGGGGTTTATCGA13390.12663926441408296No Hit
GGGCTACACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTTACTTTG13010.12304531964355633No Hit
TCATATCCCTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAAC12490.11812728995757255No Hit
GCTTAAAACTCAAAGGACCTGGCGGTGCTTCATATCCCTCTAGAGGAGCC12480.11803271246361131No Hit
CCTCATGGGCTACACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTT12360.11689778253607659No Hit
GTTACGACTTGTCTCCTCTATATAAATGCGTAGGGGTTTTAGTTAAATGT12320.11651947256023168No Hit
ATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGA12130.11472250017496838No Hit
TTACAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTG11840.11197975285009278No Hit
CTCATGGGCTACACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTTA11820.11179059786217031No Hit
ACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTT11710.11075024542859682No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC11560.10933158301917843No Hit
ATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATATGTTAAAG11500.10876411805541106No Hit
AGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAAC11460.10838580807956615No Hit
ACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACACAATAG11430.10810207559768248No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA11380.10762918812787634No Hit
ATCCACCTTCGACCCTTAAGTTTCATAAGGGCTATCGTAGTTTTCTGGGG11110.10507559579092322No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT10990.10394066586338849No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA10960.1036569333815048No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT10890.1029948909237762No Hit
GCTAAATCCACCTTCGACCCTTAAGTTTCATAAGGGCTATCGTAGTTTTC10690.10110334104455167No Hit
GATTACACATGCAAGCATCCCCATTCCAGTGAGTTCACCCTCTAAATCAC10680.10100876355059044No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)