FastQCFastQC Report
Tue 10 May 2022
EGAF00003628352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003628352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213335
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44132.068577589237584No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC8670.4064030749759768No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT8070.37827829470082264No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7050.3304661682330607No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA6750.3164037780954836No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA6360.29812267091663347No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC5730.2685916516277217No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC5020.23531066163545597No Hit
GTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA4550.21327958375325193No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4310.2020296716431903No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC4050.18984226685729017No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT3760.17624862305763236No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT3710.1739048913680362No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA3690.1729673986921977No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA3690.1729673986921977No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA3570.1673424426371669No Hit
GTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCC3550.16640494996132843No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC3280.15374879883750908No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA3240.15187381348583215No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT3230.1514050671479129No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3190.14953008179623598No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT3140.1471863501066398No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA3100.14531136475496284No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT3060.1434363794032859No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3050.14296763306536667Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG3030.14203014038952821No Hit
ATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCA2990.14015515503785128No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT2980.13968640869993204No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC2780.13031148194154735No Hit
GTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAG2770.1298427356036281No Hit
CATATGCAGATCCCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCG2680.12562401856235497No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC2660.12468652588651652No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG2580.12093655518316264No Hit
TCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGC2580.12093655518316264No Hit
GAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCA2550.11953031616940492No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA2470.11578034546605104No Hit
GCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTC2460.1153115991281318No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA2430.1139053601143741No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA2380.11156162842477792No Hit
GTACTGACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCTACATA2320.10874915039726252No Hit
GTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGAA2280.10687416504558558No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG2270.10640541870766634No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG2260.1059366723697471No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT2190.10265544800431246Clontech SMART CDS Primer II A (100% over 26bp)
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA2190.10265544800431246No Hit
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA2180.10218670166639322No Hit
GGACAAGGGATCAACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGG2180.10218670166639322No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA2170.10171795532847398No Hit
GTATATGAGCTACCGAGTCGTTCTGATAGATCCCGCGTCAGGCATATAAT2160.10124920899055477No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)