FastQCFastQC Report
Wed 11 May 2022
EGAF00003628419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003628419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9816112
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGTTTGTCCCTGCGGT737360.751173173248227No Hit
GTACATGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGG598470.6096813076297417No Hit
GCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGA516010.5256765611476316No Hit
ATGTAGTAGAGCGGCACCTCGCCACACAGGGTGTTCACGGCCATGCCCAG509070.518606552166479No Hit
GTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCT467620.47638005760325475No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTG464280.47297748843941473No Hit
TTTCTATTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT461260.46990091392600253No Hit
GCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGG453850.46235210030203405No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGTGCCACCTCTG436790.44497251050110265No Hit
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCAC392720.40007693473750094No Hit
GTACATGGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTG376420.3834715822313356No Hit
GTACATGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGG374760.38178048498224143No Hit
CCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGTTTG340320.3466953107299509No Hit
CCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCATGATGTAGCAGCAG335450.3417340796437531No Hit
GTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGTTCACGGCCA307420.31317898573284414No Hit
GAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAG291910.29737843251992235No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGCAGAGAGCATTCCATCTGG289160.29457691599280855No Hit
GTTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGC287090.29246813809785377No Hit
GGGTGTTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCT282010.2872929730223127No Hit
CTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGT269920.27497648763583793No Hit
GTCATACACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGTAA264230.26917989525791886No Hit
GGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGTATG247990.25263566674870863No Hit
GCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGTTTGTCCCT245960.2505676381850574No Hit
CCTCCGGAGGGTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTT228120.2323934364237083No Hit
GCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCAATAGAAAAGTCCACAA224010.22820644263227638No Hit
GGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAG223730.22792119731315208No Hit
CGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCACCCTCC223240.22742201800468453No Hit
TCTCCAGGGTTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTC220040.2241620715004067No Hit
TCGCCACACAGGGTGTTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCC215630.21966945772419877No Hit
AGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGTTCACGGCCATGCCC205860.2097164335533254No Hit
GTCTTGAGGCTCTCAATAGAAAAGTCCACAACTTCCAGATGGAATGCTCT204230.20805589830270887No Hit
CAATAGAAAAGTCCACAACTTCCAGATGGAATGCTCTCTGCAGGCCAAGC196840.20052745934439217No Hit
TTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGA194590.1982353094585718No Hit
GTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAGACGTAGGC192480.19608578223231357No Hit
GCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGG191670.19526060827341823No Hit
GAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTC188710.19224515775696122No Hit
GGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCA188650.192184033760006No Hit
GGGTTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAA185200.18866940393508141No Hit
GCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCTGAGTGAGCACCTG181450.1848491541253808No Hit
AGATGGATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTAC181110.1845027848093013No Hit
CTGCTGCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGG180950.1843397874840874No Hit
GTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGT178520.18186426560740138No Hit
ATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTG173520.17677059919446722No Hit
CCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGTTTGTCCCTGCGGTGT169640.17281791405803032No Hit
GGCCTGCAGAGAGCATTCCATCTGGAAGTTGTGGACTTTTCTATTGAGAG168400.17155468478762265No Hit
GGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCA162690.16573771774405183No Hit
TCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCA162180.16521816376993254No Hit
CTCCAGGGTTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCT160120.16311957320780365No Hit
GTAGTAGAGCGGCACCTCGCCACACAGGGTGTTCACGGCCATGCCCAGGA159220.16220271325347552No Hit
CCTGGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGT153360.15623293621751666No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGGAAGTTGTGGACTTTTCTA152030.1548780209516762No Hit
ATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAG148720.15150601378631376No Hit
GATTGTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAAACACACGG148020.15079290048850297No Hit
GCAAAAGACCCTTTGCCCGGGGCGTTTCCTTTCCCGTTGGGGCTTCCACT144560.14726808333075256No Hit
CATGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAG144420.14712546067119037No Hit
GGTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGTCAGGGCCCCCAG139940.14256153556520137No Hit
TCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGTTCACGGCCAT139600.14221516624912187No Hit
GCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTC138190.1407787523206744No Hit
CCCCAAGCTCCCATTTCTCCAGGGTTTGTCCCTGCGGTGTGGGCAGAAGC137330.13990264169764974No Hit
GGGCGCCACCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCC136760.13932196372657527No Hit
GTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGC135380.1379161117966054No Hit
GTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGGGGCCGATT135190.13772255247291393No Hit
CTGCTACATCATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTC133210.135705460573392No Hit
GATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGC131930.13440148197168084No Hit
GCCTGGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGC131400.13386155333190983No Hit
CCTACAAGCCAGCCCCTGGCACCTGCTGCTACATCATGAAGATAGCTCCA129310.13173240077130335No Hit
GACGTAGGCACTGCGGGCTTGGCCTGCAGAGAGCATTCCATCTGGAAGTT128470.1308766648139304No Hit
GCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGCCTGCATCTCGCC128250.1306525434917613No Hit
CTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACCCTTTGCCCGGGGCGTTT127110.1294911875496123No Hit
GGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCAT125750.12810571028529422No Hit
ACTCCGCAGCTCCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAGTGCAC123660.12597655772468774No Hit
GGGATGGACGGTCCTCCCCGCGGCGGGGTTTTCAGCCCTCGCGGGTGGGC121090.12335841318843958No Hit
CCACTGGCCTCGTGGTGTATGACTACCAGCAGCTGCTGATCGCCTACAAG118490.1207097066537138No Hit
CTCCCCAAGCTCCCATTTCTCCAGGGTTTGTCCCTGCGGTGTGGGCAGAA117390.11958910004286831No Hit
GGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCT117320.11951778871308721No Hit
ATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTGCCCAC116560.11874355141832121No Hit
CCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGGGG115320.11748032214791354No Hit
CTCCAGAACCATCTCCGTGTGTTTCTGGCTCATGTGGAGACCCATGAGCA113340.11546323024839163No Hit
ATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGTGCCACCTCTGCCCAC112180.1142814996405909No Hit
CCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAGTGCACCTGAAACGCCT111210.11329332835648166No Hit
CCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGG110150.11221347107693963No Hit
GCCTACGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCAC107130.10913689656352739No Hit
GAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGAT107040.10904521056809457No Hit
GCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGCCTGCATC106850.10885165124440309No Hit
CCTTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGG106270.10826078594050272No Hit
GGGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAG106240.1082302239420251No Hit
GCCTGGAAGTTGTGGACTTTTCTATTGAGAGCCTCAAGACTGGGGATGCT104310.10626406870663252No Hit
CATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCAATAGAA103310.1052453354240457No Hit
GTACATGGGAGCCGCAGGTTCCCCGCGCGCCCCGAGCCCCCGCGCCATGA103060.10499065210339897No Hit
GTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGAT102950.10487859144231444No Hit
GATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGGGGCCGATTTGGCA102490.10440997413232449No Hit
GTCGTGGTGATTGTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAA102480.10439978679949863No Hit
GGACTACTCCGCAGCTCCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAG102370.10428772613841407No Hit
GTGGTAACCAGGTGCTCACTCAGGGCCAGGCGTTGCTGGGCTTCCGGCGC101420.10331992951995658No Hit
GAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCT100800.10268831488475273No Hit
CCCTTTGCCCGGGGCGTTTCCTTTCCCGTTGGGGCTTCCACTGACCCTGC100400.102280821571718No Hit
GCTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACCCTTTGCCCGGGGCGTT100100.10197520158694195No Hit
CCCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGT99760.10162883227086243No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)