FastQCFastQC Report
Tue 10 May 2022
EGAF00003628461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003628461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1530958
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG54830.3581417648296034No Hit
GTACATGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG49620.3241107855342864No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG40470.2643442863880002No Hit
GTACATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAAT40160.26231941046063967No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG37580.24546721725873605No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC33720.2202542460341825No Hit
GGGTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAAT30700.200528035386993No Hit
CCATATACCAATGATGGCGCGATGTAACACGAGAAAGCACATACCAAGGC30320.1980459294115188No Hit
GTACATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC29020.18955451423226502No Hit
GTTTAGGAGTGGGACTTCTAGGGGATTTAGCGGGGTGATGCCTGTTGGGG28900.1887706912926416No Hit
CCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGC28730.18766027546150843No Hit
GAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTTCG26880.17557633847564727No Hit
GTAATAGGGCAAGGACGCCTCCTAGTTTGTTAGGGACGGATCGGAGAATT25750.16819533912752668No Hit
CTATTAGACTATGGTGAGCTCAGGTGATTGATACTCCTGATGCGAGTAAT25180.16447218016431542No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA24750.1616634812973315No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT24720.16146752556242563No Hit
GTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATA24360.15911605674355536No Hit
CCTAATGACCTCCGGCCTAGCCATGTGATTTCACTTCCACTCCATAACGC23880.15598076498506164No Hit
CCATAACGCTCCTCATACTAGGCCTACTAACCAACACACTAACCATATAC23170.15134314592562303No Hit
GGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTT22990.1501674115161879No Hit
CATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGT22740.1485344470586391No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG22670.14807721701052542No Hit
GTATTAGGATTAGGATTGTTGTGAAGTATAGTACGGATGCTACTTGTCCA22430.14650957113127858No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA22150.14468065093882393No Hit
CATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAGTAGACA21930.143243642216181No Hit
ATATTATGCTTTGTTGTTTGGATATATGGAGGATGGGGATTATTGCTAGG21540.14069621766240484No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21310.13919389036145996No Hit
GTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAATGT20890.13645051007277798No Hit
CCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCACTCATTC20640.13481754561522916No Hit
GGCCTATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCAT20230.13213948390484911No Hit
GTAATAAGCAGTGCTTGAATTATTTGGTTTCGGTTGTTTTCTATTAGACT19940.1302452451340925No Hit
ATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCC19790.12926546645956322No Hit
GTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTA19640.12828568778503394No Hit
CACACCACCTGTCCAAAAAGGCCTTCGATACGGGATAATCCTATTTATTA19520.1275018648454105No Hit
GTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA19470.12717527195390074No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA19160.12515039602654024No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT18640.12175382995483874No Hit
CTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAA18080.11809598956992941No Hit
TCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAAT17990.11750812236521184No Hit
ATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGTGTATGAGTTGG17900.11692025516049427No Hit
GATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATGCCAGTATC17800.11626706937747475No Hit
GCTCTAGAGGGGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTT17720.11574452075105914No Hit
GTGTTAATGTCATTAAGGAGAGAAGGAAGAGAAGTAAGCCGAGGGCGTCT17610.11502601638973767No Hit
GCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAAT17530.11450346776332204No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC17450.11398091913690643No Hit
ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT17290.1129358218840752No Hit
GGACTATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTC16870.11019244159539321No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT16720.10921266292086393No Hit
CCTTACACTATTCCTCATCACCCAACTAAAAATATTAAACACAAACTACC16670.10888607002935417No Hit
ATCCCATACATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTC16500.10777565419822098No Hit
GGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAG16480.10764501704161708No Hit
ATGTAACACGAGAAAGCACATACCAAGGCCACCACACACCACCTGTCCAA16450.10744906130671122No Hit
CCCCATACCCAACCCCCTGGTCAACCTCAACCTAGGCCTCCTATTTATTC16190.10575077827086046No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG16170.10562014111425656No Hit
GTCCCAATGTATGGGATGGCGGATAGTAAGTTTGTAATTACTGTGGCCCC16030.10470568101802924No Hit
GGCTATGTCCTCCCGTGAGGCCAAATATCATTCTGAGGGGCCACAGTAAT15900.10385653950010386No Hit
ACCCCTAACAGGGGCCCTCTCAGCCCTCCTAATGACCTCCGGCCTAGCCA15830.10339930945199019No Hit
GGTGAGAATAGTGTTAATGTCATTAAGGAGAGAAGGAAGAGAAGTAAGCC15720.10268080509066872No Hit
GTTTAAGGGGTTGGCTAGGGTATAATTGTCTGGGTCGCCTAGGAGGTCTG15660.10228889362085701No Hit
CCTAATTGGGGGGTAGGGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAA15460.10098252205481796No Hit
GTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAAT15370.10039465485010039No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)