FastQCFastQC Report
Wed 27 Apr 2022
EGAF00003628462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003628462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1530958
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG49930.32613566146164685No Hit
GTACATGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG40130.2621234547257338No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG39780.2598373044851655No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36760.24011109383797594No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC36220.23658389060967053No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG35820.23397114747759246No Hit
GTTTAGGAGTGGGACTTCTAGGGGATTTAGCGGGGTGATGCCTGTTGGGG33220.21698831711908492No Hit
GTACATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAAT32600.2129385652643639No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA27620.18040991326999173No Hit
GGGTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAAT27560.18001800180018002No Hit
CTATTAGACTATGGTGAGCTCAGGTGATTGATACTCCTGATGCGAGTAAT27450.17929949743885853No Hit
GAAATAGTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTTCG27150.17733994008979997No Hit
CCATATACCAATGATGGCGCGATGTAACACGAGAAAGCACATACCAAGGC26350.1721144538256438No Hit
GTAATAGGGCAAGGACGCCTCCTAGTTTGTTAGGGACGGATCGGAGAATT26270.1715919051992282No Hit
GTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA26050.17015489647658524No Hit
GTATTAGGATTAGGATTGTTGTGAAGTATAGTACGGATGCTACTTGTCCA25530.16675833040488375No Hit
CCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGC25370.1657132331520525No Hit
ATATTATGCTTTGTTGTTTGGATATATGGAGGATGGGGATTATTGCTAGG25000.16329644575488028No Hit
GTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATA24170.15787500375581826No Hit
GTACATGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC24050.15709118081619483No Hit
GGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTT23910.1561767207199675No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23210.15160442023883083No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA23040.15049400440769767No Hit
CATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAGTAGACA22240.1452685181435415No Hit
GTAATAAGCAGTGCTTGAATTATTTGGTTTCGGTTGTTTTCTATTAGACT21750.14206790780674583No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG21620.14121876628882046No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT20590.13449095272371941No Hit
CATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGT20270.1324007582180569No Hit
GTGTTAATGTCATTAAGGAGAGAAGGAAGAGAAGTAAGCCGAGGGCGTCT20230.13213948390484911No Hit
CCATAACGCTCCTCATACTAGGCCTACTAACCAACACACTAACCATATAC20170.1317475724350374No Hit
CTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAA20100.13129034238692375No Hit
GGCCTATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCAT19770.12913482930295933No Hit
CCTAATGACCTCCGGCCTAGCCATGTGATTTCACTTCCACTCCATAACGC19330.12626081185767343No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA18740.12240701573785827No Hit
CTCCTGATGCGAGTAATACGGATGTGTTTAGGAGTGGGACTTCTAGGGGA18510.12090468843691335No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG18510.12090468843691335No Hit
CCTAATTGGGGGGTAGGGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAA18190.1188144939312509No Hit
ATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGTGTATGAGTTGG18100.11822662672653333No Hit
GGACTATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTC17770.11607111364256889No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT17740.11587515790766303No Hit
GTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTA17500.11430751202841619No Hit
GTAAAGAATCGTGTGAGGGTGGGACTGTCTACTGAGTAGCCTCCTCAGAT17470.11411155629351033No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT17400.11365432624539668No Hit
CCTTACACTATTCCTCATCACCCAACTAAAAATATTAAACACAAACTACC17240.11260922899256544No Hit
CCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCACTCATTC17100.1116947688963381No Hit
GATATATGGAGGATGGGGATTATTGCTAGGATGAGGATGGATAGTAATAG17090.11162945031803616No Hit
GTCCCAATGTATGGGATGGCGGATAGTAAGTTTGTAATTACTGTGGCCCC16480.10764501704161708No Hit
GTTTAAGGGGTTGGCTAGGGTATAATTGTCTGGGTCGCCTAGGAGGTCTG16430.10731842415010731No Hit
GTACATGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTA16340.10673055694538974No Hit
TCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAAT16340.10673055694538974No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA16330.1066652383670878No Hit
ATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCC15840.10346462803029215No Hit
GCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATACGAATGGGGGC15810.10326867229538629No Hit
GTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAATGT15660.10228889362085701No Hit
AGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGTCCGTGGAAG15520.10137443352462967No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC15480.10111315921142187No Hit
ATGTAACACGAGAAAGCACATACCAAGGCCACCACACACCACCTGTCCAA15320.10006806195859064No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)