FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003628553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003628553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25
Sequences flagged as poor quality0
Sequence length100
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCCTCTCCGGCCCCGGCCGGGGCCGGAGAGGGGCTGACCGGGTTGGTT28.0No Hit
CCGGTCGTCGTCCCCCAACGCCGGTCGAGCCGCGACCGCGGCGCGCAGCC28.0No Hit
GCCATAGAAGGCCCCACCCCAGTCTCAGCCCTACTCCACTCAAGCACTAT28.0No Hit
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT14.0Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTAGGATCTCAGGCGTGAGCCACTGCACCTAGCCCGAAACCAAGCTT14.0No Hit
CATCTGAGTTGCTGCCTGTTCTTCACACTTAGCTCCAAACCCATGAAAAA14.0No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAT14.0No Hit
TGCCGAAGCCGCTGCCGCCCGCTGACTTCGGCGAACGGCGGCTTGCGTTT14.0No Hit
ATGCAACTCAAACAGCGCTCCTCGTCCGCCAGCAAAGCGTCCAGCCGCTC14.0No Hit
ATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGCTAAGACCCCCGAAA14.0No Hit
GATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATG14.0No Hit
ATGAAACTCAAACAGCGCTCCTCGTCCGCCAGCAAAGCGTCCAGCCGCTC14.0No Hit
GCAGATTGCGGTGCTGGAATCGATGAAGATGGAGCATCTGGTCGCAGCAC14.0No Hit
GATGAAGTTCGTGTACAAAGAAGAGCATCCGTTCGAGAAGCGCCGCTCTG14.0No Hit
GTCGAGCTTGTTTCTCCTGTTCTAACCCAGCCAGCCAAGCAGCTTATAAT14.0No Hit
CATCGGTCAGGAGCTTGATTTCGACAAGCTCAACCTGACGGTCGAGACCG14.0No Hit
ACCTACACCTGCATGAACACTTTCAAAGGGCGGACATAGAGACAGGATGA14.0No Hit
CTCCAGCATTACCTGCTCTCCCGCCGAGCCCGTATAGAGGGTCTGAATGT14.0No Hit
TGGCAGCACCCCGCTACGACATGGACCGCTTTGGCGTGGTCTTCCGCGCC14.0No Hit
GCTCCATCCTGGCCTCACTGTCCACCTTCCAGCAGATGTGGATTAGCAAG14.0No Hit
CAATTATCAGGTACCCTGTGGGATTCAGAGGTTTCCAAATCTTTCAGGAA14.0No Hit
CGACCTGTCGCCGGCGGACATGCTGCACATCAAGCACCTGTCTCTTATAC14.0No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)